Development of the Japanese Medaka as a Laboratory Animal with Genetic and Environmental Control.

以下结果从我们两年的“研究”中获得：1.对麝香蛋白和蛋蛋白的两种尺寸电泳分析已成功地用于识别俄里亚斯族物种的两种尺寸电泳分析。2. Medaka的hatching enzyme被发现怀疑两种不同的蛋白质、HCE和LCE，以及两种成分已被净化和表征。3. The muscle LDH-A_4 and-B_4 isozymes of the Medaka已被净化和部分特征化。4.几种可能性已经被指向了奥里齐亚斯基因中的混合不育症。5.在染色体中获得连续波段的方法已被证实使用trypsin--Giemsa（BrdU方法）。6. C波段和非波段的方法已成功地应用于O的染色体组。curvinotus and O. minutillusB。7.日本的Medaka被明确地确认对4种主要流行的看法。Nei的遗传距离估计为0.3至0.5，对于不相关变异来说，它是一个相当大的价值。8.这种折磨已经开始并继续在D-rr、Da和朝鲜半岛Medaka建立了牢固的感染线。9.该方法用于对感染性菌株进行遗传监测，并在很远的情况下被证实是通过使用多态性进行登记为8个标记酶。10. The B-Allele在F_1 progeny获得的Medaka特殊位置测试系统中发现了6个新的突变，该系统是由我们自己开发的，使用伽马射线和乙基硝基，一种潜在的化学突变体。在这些新的突变体中，眼睛表面的极度黑色素比b和b^p Poci的突变体少得多，表明所有6个突变最近孤立的甚至到b和b<@1p>@D1，可能在b位置的底部线索。11. MLR分析程序已经开发出使用金鱼的leukocytes，并且已经开始应用于Medaka。12.与Medaka在比较肿瘤学的一个观点中的特征一样好，化学地被诱导的肿瘤。

The following results were obtained from our 2 years' study :1. The two dimensional electrophoretic analysis of muscle and egg proteins was successfully used for identification of species of the genus oryzias. 2. The hatching enzyme of the Medaka was found to consist of two different proteases, HCE and LCE, and the two components were purified and characterized. 3. The muscle LDH-A_4 and ーB_4 isozymes of the Medaka were purified and partially characterized. 4. Several possibilities were pointed out with regard to the hybrid sterility in the genus Oryzias. 5. The method to obtain continuous bands in chromosomes was established using trypsinーGiemsa as well as BrdU method. 6. The C banding and NOR banding methods were successfully applied for the chromosomes of O. curvinotus and O. minutillusB. 7. The Japanese Medaka was definitively confirmed to consist of 4 major populations. The Nei's genetic distance was estimated to be 0.3 to 0.5, which was a fairly large value for intraspecies variation. 8. The attempt was started and continued to establish inbred strains in dーrr, Da and Korean Medaka. 9. The method for genetic monitoring of the inbred strains thus far obtained was established by the use of polymorphisms with regard to 8 marker enzymes. 10. The new 6 mutations at the b allele were isolated from F_1 progeny obtained by the Medaka specific-locus test system that has been developed by ourselves using gamma-rays and ethylnitrosoures, a potent chemical mutagen. In these new mutants, the extent of melanizatin of eyes body surface was less than that in the mutants at the b and b^p Poci, indicating that all the 6 mutations newly isolated were recessive even to b and b<@1p>@D1, possibly the bottom allele at the b locus. 11. The MLR assay procedure was developed using leukocytes of the goldfish, and was started being applied for the Medaka. 12. The spontaneous as well as chemically induced tumors in the Medaka were characterized from a view point of comparative oncology.