Development and Application of the Three Dimensional Microscope System for Nano Space Spectroscopy

纳米空间光谱三维显微镜系统的研制与应用

• 批准号: 11554033
• 负责人: TERAMAE Norio
• 金额: $ 9.15万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11554033/
• 关键词: Microscopy; Raman Spectroscopy; Cofocal Microscope; Photonic Crystal; 分光; ピエゾステージ; ナノ空間; 微粒子; 蛍光; ラマン散乱

In order to achieve three-dimensional chemical analyses at submicrometer spatial resolution, a new confocal Raman/fluorescent microscope coupled with close loop piezoelectric actuators for XY scanning of a sample stage and Z scanning of an objective has been developed. The closed loop operation based on reference signals from position sensors attached to the actuators provides good linearity, long time stability and high accuracy of sample displacement. Scattered light or emission from the sample is collected using the objective, and guided to a pinhole and a slit of spectrometer. Performance of this system within XY plane was checked using fluorescent microspheres and phototaic crystals made of silicon. A fluorescence image of two 200 nm spheres aggregating on a quartz glass shows that tops of both spheres are clearly resolved. Furthermore, a Raman image of the photonic crystal collected in the 500 cm-1 band of silicon shows the submicormeter holes arranged triangularly.As mentioned above, the microscope system has been developed successfully.

为了在亚微米空间分辨率下实现三维化学分析，开发了一种新的共聚焦拉曼/荧光显微镜，并与近环压电执行器相连，用于XY扫描样品阶段，并开发了Z扫描目标的Z扫描。基于连接到执行器的位置传感器的参考信号的闭环操作可提供良好的线性，长时间稳定性和样品位移的高精度。使用物镜收集样品的散射光或发射，并引导到针孔和光谱仪。使用荧光微球和由硅制成的光晶体检查了XY平面内该系统的性能。在石英玻璃上聚集的两个200 nm球的荧光图像表明，这两个球的顶部都清楚地解决了。此外，在硅的500 cm-1带中收集的光子晶体的拉曼图像显示了排列三角形的亚型计孔。如上所述，显微镜系统已成功开发出来。

项目成果

T.Ishioka,T.Uchida,N.Teramae: "Time-resolved Near-infrared Surface-enhanced Raman Spectroscopy of a Surface-confined Reaction"Bull. Chem. Soc. Jpn. 72・12. 2713-2717 (1999)

T.Ishioka，T.Uchida，N.Terame：“表面限制反应的时间分辨近红外表面增强拉曼光谱”Soc Jpn. 2713-2717。

寺前紀夫,内田達也 他4名: "界面吸着化学種の状態解析と分子認識"分析化学. 48・12. 1063-1075 (1999)

Norio Teramae、Tatsuya Uchida 等 4 人：“界面吸附化学物质的状态分析和分子识别”分析化学 48・12（1999）。