Development and Application of the Three Dimensional Microscope System for Nano Space Spectroscopy

纳米空间光谱三维显微镜系统的研制与应用

• 批准号: 11554033
• 负责人: TERAMAE Norio
• 金额: $ 9.15万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11554033/
• 关键词: Microscopy; Raman Spectroscopy; Cofocal Microscope; Photonic Crystal; 分光; ピエゾステージ; ナノ空間; 微粒子; 蛍光; ラマン散乱

In order to achieve three-dimensional chemical analyses at submicrometer spatial resolution, a new confocal Raman/fluorescent microscope coupled with close loop piezoelectric actuators for XY scanning of a sample stage and Z scanning of an objective has been developed. The closed loop operation based on reference signals from position sensors attached to the actuators provides good linearity, long time stability and high accuracy of sample displacement. Scattered light or emission from the sample is collected using the objective, and guided to a pinhole and a slit of spectrometer. Performance of this system within XY plane was checked using fluorescent microspheres and phototaic crystals made of silicon. A fluorescence image of two 200 nm spheres aggregating on a quartz glass shows that tops of both spheres are clearly resolved. Furthermore, a Raman image of the photonic crystal collected in the 500 cm-1 band of silicon shows the submicormeter holes arranged triangularly.As mentioned above, the microscope system has been developed successfully.

为了实现亚微米级空间分辨率的三维化学分析，研制了一种新型的共焦拉曼/荧光显微镜，它与闭环式压电致动器相耦合，用于样品台的XY扫描和物镜的Z扫描。基于连接在执行器上的位置传感器的参考信号的闭环操作提供了良好的线性度、长时间稳定性和高精度的样品位移。使用物镜收集样品的散射光或发射，并将其引导到光谱仪的针孔和狭缝中。利用硅基荧光微球和光电晶体对该系统在XY平面内的性能进行了检测。两个200纳米球体聚集在石英玻璃上的荧光图像显示，两个球体的顶部都被清晰地分辨出来。此外，在硅的500 cm-1波段采集的光子晶体的拉曼图像显示，亚微米级的孔洞呈三角形排列。

项目成果

T.Ishioka,T.Uchida,N.Teramae: "Time-resolved Near-infrared Surface-enhanced Raman Spectroscopy of a Surface-confined Reaction"Bull. Chem. Soc. Jpn. 72・12. 2713-2717 (1999)

T.Ishioka，T.Uchida，N.Terame：“表面限制反应的时间分辨近红外表面增强拉曼光谱”Soc Jpn. 2713-2717。

寺前紀夫,内田達也 他4名: "界面吸着化学種の状態解析と分子認識"分析化学. 48・12. 1063-1075 (1999)

Norio Teramae、Tatsuya Uchida 等 4 人：“界面吸附化学物质的状态分析和分子识别”分析化学 48・12（1999）。