Exploitation of Reaction Fields for Analytical Chemistry to Realize Specific Function for Molecule Recognition and Development of Chemical Sensing Systems

利用分析化学反应场实现分子识别的特定功能和化学传感系统的开发

• 批准号: 14204074
• 负责人: TERAMAE Norio
• 金额: $ 35.86万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14204074/
• 关键词: solid; loqiod interface; liquid; liquid interface; meso-material; Anodized Alumina membrane; Molecule Recognition; Fluorescent Ligands; 界面イオン移動; 水素結合レセプター; メソポーラス; ナノ複合体; 化学センサー; MCM-41; メソポーラスシリカ; 無機・有機ナノ複合体; 界面分子認識; サイクリックボルタンメトリー

In combination with abasic site (AP site)-containing oligodeoxynucleotides (ODNs), a potential use of a hydrogen bond forming ligand, 2-amino-7-methyl-1,8-naphthyridine (AMND), has been demonstrated for the fluorescence detection of the cytosine (C)/guanine (G) mutation sequence of the cancer repression gene p53. An AP site-containing ODN is hybridized with a target ODN so as to place the AP site toward a target nucleobase, by which hydrophobic microenvironments are provided for ligands to recognize target nucleobases through hydrogen-bonding. To increase the binding constant, a methyl group was attached to AMND and its detection ability for SNPs was examined. As a result, high affinity was achieved as expected. The effect of the structure of a hydrophobic site in an oligo-DNA duplex on the affinity was also examined.Potential use of a surfactant-like receptor is demonstrated at the 1,2-dichloroethane-water interface for strong and selective binding of H_2PO_4^-over Br^-and Cl^-. The a … More nalysis by interfacial tensiometry reveals that the interfacial adsorption of a thiourea-isothiouronium conjugate, BT-C1, is significantly stabilized by the binding of H_2PO_4^-with the adsorption constant of 1.7x10^5 M^<-1>, while the interfacial adsorptivity of this receptor is relatively moderate for Br^-and Cl^-. Such complexation-induced interfacial adsorption behaviors of BT-C1 are discussed as a basis for the development of receptor-based chemical sensors for phosphate anions A mesoporous membrane composed of nanochannels with a uniform diameter has a potential use for precise size-exclusive separation of molecules. Here, a novel method is reported to form a hybrid membrane composed of silica-surfactant nanocomposite and a porous alumina membrane, by which size-selective transport of molecules across the membrane becomes possible. The nanocomposite formed inside each columnar with nanocompositesAlumina pore was an assembly of surfactant-templated silica-nanochannels with a channel diameter of 3.4 nm ; the channel direction being predominantly oriented along the wall of the columnar alumina pore. Molecules could be transported across the membrane including the silica-surfactant nanocomposite with a capability of nanometer-order size-exclusive separation. Our proposed membrane system has a potential use not only for separation science, but also catalysis and chip technologies. Less

与含脱碱基位点（AP site）的寡脱氧核苷酸（ODNs）结合，氢键形成配体2-氨基-7-甲基-1，8-萘啶（AMND）已被证明可用于荧光检测癌症抑制基因p53的胞嘧啶（C）/鸟嘌呤（G）突变序列。将含有AP位点的ODN与靶ODN杂交，使AP位点朝向靶核碱基，从而为配体提供疏水微环境，以通过氢键结合识别靶核碱基。为了增加结合常数，将甲基连接到AMND上，并检查其对SNP的检测能力。结果，如预期的那样实现了高亲和力。在1，2-二氯乙烷-水界面上，H_2PO_4^-与Br^-和Cl^-的选择性结合可能是一种表面活性剂样受体。的a ...更多信息 界面张力分析表明，H_2PO_4^-对硫脲-异硫脲偶联物BT-C1的界面吸附有明显的稳定作用，其吸附常数为1.7 × 10^5 M^-<-1>，而对Br^-和Cl^-的界面吸附相对较弱。这种络合诱导的界面吸附行为的BT-C1进行了讨论，作为开发基于受体的化学传感器的磷酸根阴离子的基础。由具有均匀直径的纳米通道组成的介孔膜具有精确的尺寸排他的分子分离的潜在用途。在这里，报道了一种新的方法来形成由二氧化硅-表面活性剂纳米复合物和多孔氧化铝膜组成的混合膜，通过该混合膜，分子的尺寸选择性跨膜运输成为可能。纳米复合物氧化铝孔内形成的每个柱状纳米复合物是一个组件的表面模板二氧化硅纳米通道的通道直径为3.4 nm的通道，通道的方向主要是定向沿着的柱状氧化铝孔的壁。分子可以通过膜传输，包括二氧化硅-表面活性剂纳米复合材料具有纳米级尺寸的排他性分离能力。我们提出的膜系统不仅在分离科学，而且在催化和芯片技术中具有潜在的用途。少

项目成果

Akira Yamaguchi, Ryo Kato, Seiichi Nishizawa, Norio Teramae: "Anion Recognition at the Solid/Liquid Interface as Studied by Second Harmonic Generation Spectroscopy"Chemistry Letters. 32・9. 798-799 (2003)

Akira Yamaguchi、R​​yo Kato、Seiichi Nishizawa、Norio Teramae：“通过二次谐波产生光谱研究的固体/液体界面的阴离子识别”化学快报 32・9（2003）。

Thiourea-isothiouronium conjugate for strong and selective binding of very hidrophilic H2PO4- anion at the 1,2-dichloroethane-water interface

硫脲-异硫脲缀合物，可在 1,2-二氯乙烷-水界面上强效且选择性地结合亲水性 H2PO4- 阴离子

• 发表时间: 2004
• 期刊: Tetrahedron Lett. 45・22
• 作者: Y.Sekiguchi;他4名;D.Wang;R.Kato et al.
• 通讯作者: R.Kato et al.

Electrochemical modification of benzo-15-crown-5 ether on a glassy carbon electrode for alkali metal cation recognition

• DOI: 10.1016/j.jelechem.2003.09.018
• 发表时间: 2004-03-01
• 期刊: JOURNAL OF ELECTROANALYTICAL CHEMISTRY
• 影响因子: 4.5
• 作者: Morita, K;Yamaguchi, A;Teramae, N
• 通讯作者: Teramae, N

Fluorescence detection of cytosine/guanine transversion based on a hydrogen bond forming ligand

• DOI: 10.1016/j.talanta.2003.09.027
• 发表时间: 2004-05-10
• 期刊: TALANTA
• 影响因子: 6.1
• 作者: Nishizawa, S;Yoshimoto, K;Teramae, N
• 通讯作者: Teramae, N

S.Nishizawa, Y.-Y.Cui, M.Minagawa, K.Morita, Y.Kato, S.Taniguchi, R.Kato, N.Teramae: "Conversion of thioureas to fluorescent isothiouroniumu-based photoinduced electron transfer sensors for oxoanion sensing"J. Chem. Soc., Perkin Trans. 2. 5号. 866-870 (200

S.Nishizawa、Y.-Y.Cui、M.Minakawa、K.Morita、Y.Kato、S.Taniguchi、R​​.Kato、N.Teramae：“将硫脲转化为基于荧光异硫脲基光诱导电子转移传感器的含氧阴离子传感”J. Chem. Soc.，Perkin Trans. 2. No. 5. 866-870 (200