Exploitation of Reaction Fields for Analytical Chemistry to Realize Specific Function for Molecule Recognition and Development of Chemical Sensing Systems

与含有碱性位点（AP位点）的寡脱氧核苷酸（ODNs）结合，氢键形成配体2-氨基-7-甲基-1,8-萘啶（AMND）的潜在用途已被证明用于荧光检测癌症抑制基因p53的胞嘧啶(C)/鸟嘌呤(G)突变序列。将含有AP位点的ODN与靶ODN杂交，使AP位点朝向靶核碱基，从而为配体通过氢键识别靶核碱基提供疏水微环境。为了提高AMND的结合常数，在AMND上加入一个甲基，并检测其对snp的检测能力。结果，达到了预期的高亲和力。还研究了寡聚dna双链中疏水位点的结构对亲和性的影响。表面活性剂样受体在1,2-二氯乙烷-水界面上的潜在用途是H_2PO_4^-在Br^和Cl^-上的强和选择性结合。界面张力分析表明，硫脲-异硫脲偶联物BT-C1在H_2PO_4^-的作用下具有明显的界面吸附稳定性，吸附常数为1.7x10^5 M^<-1>，而该受体对Br^和Cl^-的界面吸附能力相对较弱。本文讨论了这种络合诱导的BT-C1的界面吸附行为，为开发基于受体的磷酸阴离子化学传感器奠定了基础。由直径均匀的纳米通道组成的介孔膜具有精确的分子分离的潜在用途。本文报道了一种由二氧化硅-表面活性剂纳米复合材料和多孔氧化铝膜组成的杂化膜的新方法，通过这种方法，分子在膜上的大小选择性运输成为可能。氧化铝孔是由表面活性剂模板化的二氧化硅纳米通道组成，通道直径为3.4 nm；通道方向主要沿柱状氧化铝孔壁取向。包括二氧化硅-表面活性剂纳米复合材料在内的分子可以跨膜运输，具有纳米级尺寸的分离能力。我们提出的膜系统不仅在分离科学，而且在催化和芯片技术方面具有潜在的用途。少

In combination with abasic site (AP site)-containing oligodeoxynucleotides (ODNs), a potential use of a hydrogen bond forming ligand, 2-amino-7-methyl-1,8-naphthyridine (AMND), has been demonstrated for the fluorescence detection of the cytosine (C)/guanine (G) mutation sequence of the cancer repression gene p53. An AP site-containing ODN is hybridized with a target ODN so as to place the AP site toward a target nucleobase, by which hydrophobic microenvironments are provided for ligands to recognize target nucleobases through hydrogen-bonding. To increase the binding constant, a methyl group was attached to AMND and its detection ability for SNPs was examined. As a result, high affinity was achieved as expected. The effect of the structure of a hydrophobic site in an oligo-DNA duplex on the affinity was also examined.Potential use of a surfactant-like receptor is demonstrated at the 1,2-dichloroethane-water interface for strong and selective binding of H_2PO_4^-over Br^-and Cl^-. The a … More nalysis by interfacial tensiometry reveals that the interfacial adsorption of a thiourea-isothiouronium conjugate, BT-C1, is significantly stabilized by the binding of H_2PO_4^-with the adsorption constant of 1.7x10^5 M^<-1>, while the interfacial adsorptivity of this receptor is relatively moderate for Br^-and Cl^-. Such complexation-induced interfacial adsorption behaviors of BT-C1 are discussed as a basis for the development of receptor-based chemical sensors for phosphate anions A mesoporous membrane composed of nanochannels with a uniform diameter has a potential use for precise size-exclusive separation of molecules. Here, a novel method is reported to form a hybrid membrane composed of silica-surfactant nanocomposite and a porous alumina membrane, by which size-selective transport of molecules across the membrane becomes possible. The nanocomposite formed inside each columnar with nanocompositesAlumina pore was an assembly of surfactant-templated silica-nanochannels with a channel diameter of 3.4 nm ; the channel direction being predominantly oriented along the wall of the columnar alumina pore. Molecules could be transported across the membrane including the silica-surfactant nanocomposite with a capability of nanometer-order size-exclusive separation. Our proposed membrane system has a potential use not only for separation science, but also catalysis and chip technologies. Less