Exploitation of Reaction Fields for Analytical Chemistry to Realize Specific Function for Molecule Recognition and Development of Chemical Sensing Systems

利用分析化学反应场实现分子识别的特定功能和化学传感系统的开发

• 批准号: 14204074
• 负责人: TERAMAE Norio
• 金额: $ 35.86万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14204074/
• 关键词: solid; loqiod interface; liquid; liquid interface; meso-material; Anodized Alumina membrane; Molecule Recognition; Fluorescent Ligands; 界面イオン移動; 水素結合レセプター; メソポーラス; ナノ複合体; 化学センサー; MCM-41; メソポーラスシリカ; 無機・有機ナノ複合体; 界面分子認識; サイクリックボルタンメトリー

In combination with abasic site (AP site)-containing oligodeoxynucleotides (ODNs), a potential use of a hydrogen bond forming ligand, 2-amino-7-methyl-1,8-naphthyridine (AMND), has been demonstrated for the fluorescence detection of the cytosine (C)/guanine (G) mutation sequence of the cancer repression gene p53. An AP site-containing ODN is hybridized with a target ODN so as to place the AP site toward a target nucleobase, by which hydrophobic microenvironments are provided for ligands to recognize target nucleobases through hydrogen-bonding. To increase the binding constant, a methyl group was attached to AMND and its detection ability for SNPs was examined. As a result, high affinity was achieved as expected. The effect of the structure of a hydrophobic site in an oligo-DNA duplex on the affinity was also examined.Potential use of a surfactant-like receptor is demonstrated at the 1,2-dichloroethane-water interface for strong and selective binding of H_2PO_4^-over Br^-and Cl^-. The a … More nalysis by interfacial tensiometry reveals that the interfacial adsorption of a thiourea-isothiouronium conjugate, BT-C1, is significantly stabilized by the binding of H_2PO_4^-with the adsorption constant of 1.7x10^5 M^<-1>, while the interfacial adsorptivity of this receptor is relatively moderate for Br^-and Cl^-. Such complexation-induced interfacial adsorption behaviors of BT-C1 are discussed as a basis for the development of receptor-based chemical sensors for phosphate anions A mesoporous membrane composed of nanochannels with a uniform diameter has a potential use for precise size-exclusive separation of molecules. Here, a novel method is reported to form a hybrid membrane composed of silica-surfactant nanocomposite and a porous alumina membrane, by which size-selective transport of molecules across the membrane becomes possible. The nanocomposite formed inside each columnar with nanocompositesAlumina pore was an assembly of surfactant-templated silica-nanochannels with a channel diameter of 3.4 nm ; the channel direction being predominantly oriented along the wall of the columnar alumina pore. Molecules could be transported across the membrane including the silica-surfactant nanocomposite with a capability of nanometer-order size-exclusive separation. Our proposed membrane system has a potential use not only for separation science, but also catalysis and chip technologies. Less

结合含有碱性位点(AP位点)的寡脱氧核苷酸(ODN)，氢键形成配体2-氨基-7-甲基-1，8-萘啶(AMND)已被证明用于荧光检测抑癌基因P53的胞嘧啶(C)/鸟氨酸(G)突变序列。含有AP位点的ODN与靶ODN杂交，使AP位点朝向靶核苷酸碱基，从而为配体通过氢键识别靶核苷酸碱基提供疏水微环境。为了提高结合常数，在AMND上连接了一个甲基，并考察了其对SNPs的检测能力。结果，达到了预期的高亲和力。在1，2-二氯乙烷-水界面上，表面活性剂样受体的潜在用途是在Br^-和Cl^-上与H_2PO_4^-强烈和选择性地结合。The a…进一步的界面张力计分析表明，H_2PO_4~-对硫脲-异硫金共轭化合物BT-C_1的界面吸附有显著的稳定作用，其吸附常数为1.7×10~(-5)M^~(-1)，而该受体的界面吸附能力对Br~-和Cl~(-)的吸附能力相对较弱。这种络合诱导的界面吸附行为是开发基于受体的磷酸盐阴离子化学传感器的基础。由直径均匀的纳米孔道组成的介孔膜在分子的精确尺寸排斥分离方面具有潜在的应用前景。在这里，报道了一种新的方法来形成由二氧化硅-表面活性剂纳米复合材料和多孔氧化铝膜组成的杂化膜，通过这种杂化膜可以实现分子的尺寸选择性传递。在每个柱状氧化铝孔内形成的纳米复合材料是以表面活性剂为模板的二氧化硅纳米通道的集合，通道直径为3.4 nm，通道方向主要沿柱状氧化铝孔壁取向。分子可以跨膜传输，包括具有纳米级尺寸排除性分离能力的二氧化硅-表面活性剂纳米复合材料。我们提出的膜系统不仅在分离科学上有潜在的用途，而且在催化和芯片技术中也有潜在的用途。较少

项目成果

Akira Yamaguchi, Ryo Kato, Seiichi Nishizawa, Norio Teramae: "Anion Recognition at the Solid/Liquid Interface as Studied by Second Harmonic Generation Spectroscopy"Chemistry Letters. 32・9. 798-799 (2003)

Akira Yamaguchi、R​​yo Kato、Seiichi Nishizawa、Norio Teramae：“通过二次谐波产生光谱研究的固体/液体界面的阴离子识别”化学快报 32・9（2003）。

Thiourea-isothiouronium conjugate for strong and selective binding of very hidrophilic H2PO4- anion at the 1,2-dichloroethane-water interface

硫脲-异硫脲缀合物，可在 1,2-二氯乙烷-水界面上强效且选择性地结合亲水性 H2PO4- 阴离子

• 发表时间: 2004
• 期刊: Tetrahedron Lett. 45・22
• 作者: Y.Sekiguchi;他4名;D.Wang;R.Kato et al.
• 通讯作者: R.Kato et al.

Electrochemical modification of benzo-15-crown-5 ether on a glassy carbon electrode for alkali metal cation recognition

• DOI: 10.1016/j.jelechem.2003.09.018
• 发表时间: 2004-03-01
• 期刊: JOURNAL OF ELECTROANALYTICAL CHEMISTRY
• 影响因子: 4.5
• 作者: Morita, K;Yamaguchi, A;Teramae, N
• 通讯作者: Teramae, N

Fluorescence detection of cytosine/guanine transversion based on a hydrogen bond forming ligand

• DOI: 10.1016/j.talanta.2003.09.027
• 发表时间: 2004-05-10
• 期刊: TALANTA
• 影响因子: 6.1
• 作者: Nishizawa, S;Yoshimoto, K;Teramae, N
• 通讯作者: Teramae, N

S.Nishizawa, Y.-Y.Cui, M.Minagawa, K.Morita, Y.Kato, S.Taniguchi, R.Kato, N.Teramae: "Conversion of thioureas to fluorescent isothiouroniumu-based photoinduced electron transfer sensors for oxoanion sensing"J. Chem. Soc., Perkin Trans. 2. 5号. 866-870 (200

S.Nishizawa、Y.-Y.Cui、M.Minakawa、K.Morita、Y.Kato、S.Taniguchi、R​​.Kato、N.Teramae：“将硫脲转化为基于荧光异硫脲基光诱导电子转移传感器的含氧阴离子传感”J. Chem. Soc.，Perkin Trans. 2. No. 5. 866-870 (200