Phytogeographical relationship between Madaqascar and Southeast Asia

马达卡斯加与东南亚的植物地理关系

• 批准号: 13575012
• 负责人: MURAKAMI Noriaki
• 金额: $ 8.45万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13575012/
• 关键词: geographical distribution; wind dispersal; ferns; phytogeography; long distance dispersal; molecular data; Madagascar; Malaysia

We got several molecular data suggesting that several fern species growing in tropical rain forests of Madagascar originated from Southeast Asia by long distance dispersal. There might exist recurrent gene flow by wind spore dispersal. In this research project, in order to test the hypothesis, we' tried to compare genetic identities of several fern species as well as some angiosperm species with very small seeds between Madagascar and Southeast Asia (Malaysia and Indonesia). We collected the same or closely related plant species such as Asplenium nidus complex, Hymenasplenium unilaterale complex., Nepenthes, Tacca and Dandelion species both from the two distant regions and determined their nucleotide sequences of chloroplast genes such as rbcL, and analyzed allozyme variations coded by nuclear genes. We also observed and compared pollen or spore morphology using SEM. The obtained data strongly suggested that plant species with small spores or seed which are suitable for long distance dispersal by wind, had gene exchange frequently between Madagascar and Southeast Asia which are 6,000 km apart to each other. Thus, close relationship between the two regions was indicated by this research project.

我们得到的一些分子数据表明，马达加斯加热带雨林中生长的几种蕨类植物是通过远距离传播起源于东南亚的。可能存在风孢子传播的轮回基因流。在本研究项目中，为了验证这一假设，我们试图比较马达加斯加和东南亚（马来西亚和印度尼西亚）之间的几种蕨类植物物种以及一些种子非常小的被子植物物种的遗传特性。我们收集了相同或相近的植物种，如巢状铁角蕨复合体、单侧膜角蕨复合体。猪笼草属（Nepenthes）、塔卡属（Tacca）和丹顶鹤属（Danelae）3种植物的叶绿体基因rbcL的核苷酸序列测定，并分析了核基因编码的等位酶变异。利用扫描电镜对花粉或孢子形态进行了观察和比较。所获得的数据有力地表明，具有小孢子或种子的植物物种适合于通过风进行长距离传播，马达加斯加和东南亚之间的基因交流频繁，这两个国家相距6,000公里。因此，这两个地区之间的密切关系表明了本研究项目。

项目成果

Yatabe, Y., Murakami, N.: "Recognition of cryptic species in the Asplenium nidus complex using molecular data - a progress report"Telopea. 10・1. 487-496 (2003)

Yatabe，Y.，Murakami，N.：“利用分子数据识别铁角蕨复合体中的神秘物种 - 进展报告”Telopea 10・1（2003）。

Yatabe, Y.Murakami, N: "Recognition of cryptic species in the Asplenium nidus complex using molecular data-a progress report."Telopea. 10. 487-496 (2003)

Yatabe，Y.Murakami，N：“利用分子数据识别铁角巢复合体中的隐秘物种——进展报告。”Telopea。

Aoki, K., Suzuki, T., Hsu, T.Murakami, N: "Phylogegraphy of the component species of broad-leaved evegreen forests in Japan."Journal of Plant Research. 117. 77-94 (2004)

Aoki, K.、Suzuki, T.、Hsu、T.Murakami, N：“日本阔叶常绿林组成物种的系统发育。”植物研究杂志。

Yatabe, Y., Darnaedi, D.Murakami, N: "Allozyme Analysis of cryptic species in the As ;lenium nidus complex from West Java, Indonesia."Journal of Plant Research. 115. 484-490 (2002)

Yatabe，Y.，Darnaedi，D.Murakami，N：“来自印度尼西亚西爪哇的As ；lenium nidus复合物中隐秘物种的同种酶分析。”植物研究杂志。

Yatabe, Y., Darnaedi, D., N.Murakami: "Allozyme analysis of cryptic species in the A. nidus complex from West Java, Indonesia"Journal of Plant Research. 115. 483-490 (2002)

Yatabe，Y.，Darnaedi，D.，N.Murakami：“来自印度尼西亚西爪哇的巢巢复合体中隐秘物种的同种酶分析”植物研究杂志。