Construction of inducing disease resistance by Heteroconium chaetospira

毛螺异孢菌诱导抗病的构建

• 批准号: 14360025
• 负责人: HASHIBA Teruyoshi
• 金额: $ 9.54万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360025/
• 关键词: root endophytic fungus; Heteroconium chaetospira; Chinese cabbage; systemic resistance; effective biocontrol agent; DNAマイクロアレイ; フェニルプロパノイド; カマレキシン; H.chaetospira; 病害抵抗性誘導; MAPキナーゼ; コリスミ酸; フェノール性化合物; サリチル酸; フェニルプロパノイド経路; 誘導抵抗性

Root endophytic fungus Heteroconium chaetospiura is known to induce strong antagonistic responses in host plants and the phenomenon can be applied to provide resistance to different species of plants and pathogens. We found that, when used as a preinoculum, H.chaetospira suppressed the incidence of clubroot and Verticillium yellows when test plants were post-inoculated with the causal agents of these diseases.Furthermore, colonization of roots by H.chaetospira could induce systemic resistance in Chinese cabbage and reduce the incidence of bacterial leaf spot and Alternaria leaf spot. H.chaetospira penetrates through the outer epidermal cells of its host, passes into the inner conxtex, and grows throughout the cortical cells, including those of the root tip region. H.chaetospira can grow and persist for long periods and may be less affected by microbial interactions outside the root then other biocontrol agents. Basic environmental conditions, including temperature, moisture, sunlight, and soil physical and chemical characteristics, can greatly affect the physiology of the host plant and subsequent disease development, as well as alter interactions among the plant, pathogen, and biocontrol agents in various ways. H.chaetospira is an effective bioconrol agent against clubroot in Chainese cabbage at low to moderate soil moisture range and a pathogen resting spore density of 10 resting spores per gram of soil in situ.

已知根内生真菌Heteroconium chaetospiura在宿主植物中诱导强烈的拮抗反应，并且该现象可用于提供对不同物种的植物和病原体的抗性。结果表明，以毛螺旋体作为预接种物，对根肿病和黄化病的发生有抑制作用;同时，毛螺旋体在大白菜根系定殖，可诱导大白菜产生系统抗性，降低细菌性叶斑病和链格孢叶斑病的发生。毛螺旋体穿透宿主的外表皮细胞，进入内丛，并在整个皮层细胞中生长，包括根尖区域的皮层细胞。毛螺旋体可以生长并持续很长一段时间，并且可能比其他生物控制剂更少受到根外微生物相互作用的影响。基本环境条件，包括温度、湿度、阳光和土壤物理和化学特性，可以极大地影响宿主植物的生理和随后的疾病发展，以及以各种方式改变植物、病原体和生防剂之间的相互作用。毛螺旋体是一种有效的生防剂，在低至中等土壤湿度范围内，病原菌的休眠孢子密度为10个休眠孢子/克土壤。

项目成果

S.Morita: "Induced systemic resistance of Chinese cabbage to bacterial leaf spot and Altemaria leaf spot by the root endophytic fungus, Heteroconium chaetospira"J. General Plant Pathos.. 69. 71-75 (2003)

S.Morita：“通过根部内生真菌 Heterocium chaetospira 诱导大白菜对细菌性叶斑病和链格孢叶斑病的系统抗性”J。

Effects of environmental conditions on the biological control of clubroot in Chinese cabbage by root endophytic fungus H.chaetospira

环境条件对根内生真菌毛旋菌生物防治大白菜根肿病的影响

• 发表时间: 2005
• 期刊: Plant Dis. 89(3)
• 作者: Yokotani N.;Tamura S.;Nakano R.;Inaba A.;McGlasson B.W.;Kubo Y.;K.Narisawa et al.
• 通讯作者: K.Narisawa et al.

Developments in Plant Protection-Bridging Bioscience

植物保护桥接生物科学的发展

• 发表时间: 2005
• 期刊: Softscience, Inc.
• 作者: Hiwasa K.;Nakano R.;Hashimoto A.;Matsuzaki M.;Murayama H.;Inaba A.;Kubo Y.;T.Hashiba et al.
• 通讯作者: T.Hashiba et al.

Bio-frontier of Recognition and Response in Plant-Microbe Interactions

植物-微生物相互作用中识别和响应的生物前沿

• 发表时间: 2004
• 期刊: PSJ Plant-Microbe Interactions Symposium
• 作者: Yokotani N.;Tamura S.;Nakano R.;Inaba A.;Kubo Y.;S.Matsumoto;T.Hashiba et al.
• 通讯作者: T.Hashiba et al.

K.Narisawa: "Ultrastructural study on interaction between a sterile, white endophytic fungus and eggplant roots"J. General Plant Pathol. (in press). (2003)

K.Narisawa：“无菌白色内生真菌与茄子根之间相互作用的超微结构研究”J.