Studies on suppressive mechanisms against soil borne diseases due to the root endophytic fungus, Heteroconium chaetospira.

根部内生真菌 Heterocium chaetospira 抑制土传病害机制的研究。

• 批准号: 11460018
• 负责人: HASHIBA Teruyoshi
• 金额: $ 8.7万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460018/
• 关键词: Heteroconium chaetospira; Symbiosis in plants; Induction of systemic resistance; root endophyte; Salicylic acid; PR-1a; PAL; Suppression of symptoms against A. brassicae; 黒班病菌; PDF1.2; 地上部への抵抗性の誘導; 宿主植物根への定着; エチレンの生成; エチレン応答

Root-colonizing fungi were isolated from a total of 663 root segments of Chinese cabbage plants grown in soils collected from wheat, rape, Chinese cabbage, and napier grass fields. Sixteen isolates almost completely suppressed clubroot in sterile soil. Amongst these 16 isolates, two from Heteroconium chaetospira were also effective in nonsterile soil. Chinese cabbage seedlings from seed treated with these two isolates appeared healthy, and inoculation with one isolate promoted growth. Hyphae of the fungus covered the root surface and extensively colonized the inner cortical tissues.The root endophytic fungus, Heteroconium chaetospira isolate OGR-3, was tested for its ability to induce systemic resistance in Chinese cabbage against bacterial leaf spot caused by Pseudomonas syringae pv. macricola and Alternaria leaf spot caused by Alternaria brassicae. Chinese cabbage seedlings inoculated with the isolate of H. chaetospira were incubated in a growth chamber for 32 days. The induced seedlings were challenge inoculated with P. syringae pv. macuricola or A. brassicae. The inoculated Chinese cabbage showed a significant decrease in the lesion number of bacterial leaf spot or Alternaria leaf spot when compared to the non-treated control plants. This indicated that H. chaetospira can induce systemic resistance on foliar by infecting roots.

从小麦、油菜、大白菜和纳皮耶草地土壤中采集的663株大白菜根段中分离到定殖真菌。16个菌株几乎完全抑制根肿病在无菌土壤。在这16个菌株中，两个来自Heteroconium chaetospira的菌株在非无菌土壤中也有效。这两个菌株处理的大白菜幼苗表现出健康，其中一个菌株接种促进生长。利用根内生真菌Heteroconium chaetospira isolate OGR-3诱导大白菜对细菌性叶斑病的抗性，并对其进行了室内盆栽试验。由芸苔链格孢（Alternaria brassicae）引起的斑点落叶病（Alternaria leaf spot）。用H.在生长室中培养毛螺旋体32天。将诱导的幼苗挑战接种P. macuricola或A.芸苔属。与未处理的对照相比，接种的大白菜表现出细菌性叶斑病和链格孢叶斑病的病斑数量显著减少。这表明H.毛螺旋体可以通过感染根部在叶片上诱导系统抗性。

项目成果

Hashiba, T., et al.: "Suppression of Verticillium wilt in eggplant by some fungal root endophytes."European Journal of Plant Pathology. (in press). (2002)

Hashiba, T. 等人：“一些根内生真菌对茄子黄萎病的抑制。”欧洲植物病理学杂志。

Hashiba, T., et al.: "Protecction of Chinese Caggage against Bacterial Leaf Spot and Alternaria Leaf Spot by the Root Endophytic Fungus, Heteroconium chaetospira"Journal of General Plant Pathology. 68(in press). (2002)

Hashiba, T., et al.：“根内生真菌 Heterocium chaetospira 保护中国箱包免受细菌性叶斑病和链格孢叶斑病”普通植物病理学杂志。

Narisawa, K., Ohki, T., Hashiba, T.: "Root endophytic fungus, Heteroconium chaetospira controls clubroot disease and yellows of Chinese cabbage in the field"Plant Pathology. 49・1. 141-146 (2000)

Narisawa, K., Ohki, T., Hashiba, T.：“根内生真菌，异旋菌控制大白菜的根肿病和黄化”植物病理学 49・1（2000）。

森田聡一郎 ら: "東北地方における植物病理学のフロントライン"羽柴輝良,高橋英樹 編. 302 (2000)

森田宗一郎等：“东北地区植物病理学的前线”，桥场辉义和高桥英树编辑 302 (2000)。

Hashiba, T., Sasaki, A., Katsura, K: "Major Fungal Diseases of Rice Recent Advances"Kluwer Academic Publishers. 365 (2001)

Hashiba, T.、Sasaki, A.、Katsura, K：“水稻主要真菌病害的最新进展”Kluwer 学术出版社。