Mass production of recombinant fish gonadotropins using fish as a bioreactor

以鱼为生物反应器大规模生产重组鱼促性腺激素

• 批准号: 14360107
• 负责人: KOBAYASHI Makito
• 金额: $ 9.54万
• 依托单位: International Christian University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360107/
• 关键词: gonadotropin; recombinant hormone; rainbow trout; silkworm; goldfish; 組換えタンパク質

1.Production of recombinant goldfish gonadotropins (GTHs) by silkworm laravae1)Two types of GTHs, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), could be produced by vaculovirus silkworm larvae system.2)Both recombinant FSH and LH stimulated testosterone (T) production in male goldfish and estradiol (B) in female goldfish in vivo.3)Single-chain GTHs and GTHs with equine C-terminal peptide were produced in silkworm larvae. Both FSH and LH of the recombinant GTHs stimulated milt production in male goldfish.2.Production of recombinant goldfish GTHs by rainbow trout embryo.1)Recombinant goldfish GTHs could be produced by 4-day old rainbow trout embryo. Both FSH and LH stimulated T production in the testis and T and E production in the ovary of goldfish in vitro.2)Recombinant LH with Histidine-tag could be purified by Ni-column approximately 120 times. The purified LH stimulated T production in the goldfish testis in vitro.

1.家蚕幼虫生产重组金鱼促性腺激素（GTH）1）两种类型的GTH，卵泡刺激素（FSH）和黄体生成素（LH），重组FSH和LH均能刺激雄性金鱼体内睾酮（T）和雌性金鱼体内雌二醇（B）的产生。3）单链GTH和含马C-蚕幼虫中产生末端肽。重组GTH的FSH和LH均能刺激雄性金鱼产精; 2.虹鳟胚胎生产重组金鱼GTH：1）4日龄虹鳟胚胎可生产重组金鱼GTH。FSH和LH均能刺激金鱼睾丸中T的产生和卵巢中T、E的产生。2）重组LH经镍柱纯化后，纯度可达120倍左右。纯化的LH在体外刺激金鱼睾丸产生T。

项目成果

Kobayashi et al.: "Production of recombinant goldfish gonadotropins by baculovirus in silkworm larvae."Fish Physiology and Biochemistry. (印刷中). (2004)

Kobayashi 等人：“通过蚕幼虫中的杆状病毒生产重组金鱼促性腺激素。”鱼类生理学和生物化学（2004 年出版）。

Morita, T.: "Production of biologically-active recombinant goldfish gonadotropins in transgenic rainbow trout."Fish Physiol.Biochem.. 印刷中.

Morita, T.：“在转基因虹鳟鱼中生产具有生物活性的重组金鱼促性腺激素。”Fish Physiol.Biochem.. 正在出版。

Kobayashi, M.: "Production of recombinant goldfish gonadotropins by baculovirus in silkworm larvae."Fish Physiol.Biochem.. (in press).

Kobayashi, M.：“通过蚕幼虫中的杆状病毒生产重组金鱼促性腺激素。”Fish Physiol.Biochem..（出版中）。

Morita, T.: "Production of biologically-active recombinant goldfish gonadotropins in transgenic rainbow trout."Fish Physiol.Biochem.. (in press).

Morita, T.：“在转基因虹鳟鱼中生产具有生物活性的重组金鱼促性腺激素。”Fish Physiol.Biochem..（正在出版）。

Kobayashi, M.: "Production of recombinant goldfish gonadotropins by baculovirus in silkworm larvae."Fish Physiol.Biochem.. 印刷中.

Kobayashi, M.：“通过蚕幼虫中的杆状病毒生产重组金鱼促性腺激素。”鱼类生理学.生物化学..正在出版。