Production and provision of recombinant fish gonadotropins

利用杆状病毒家蚕幼虫系统生产重组鱼促性腺激素--卵泡刺激素和促黄体激素。我们生产金鱼、东北鲑鱼和日本鳗鱼促性腺激素。金鱼和鲑鱼促性腺激素的生物活性通过体内和体外生物测定得到证实：诱导性类固醇的产生、精子发生、产奶和排卵。利用杆状病毒家蚕幼虫系统获得的重组激素产量远高于以往报道的其他系统，如利用酵母、阿米巴、昆虫细胞等系统。本研究提纯了鲑鱼促性腺激素，并提供给其他研究小组进行研究：日本的两个小组，美国、荷兰和以色列的小组。本研究结果表明，我们的重组鱼类促性腺激素可用于鱼类繁殖的基础研究，也可用于诱导水产养殖鱼性腺成熟。

Recombinant fish gonadotropins, follicle-stimulating hormone and lutenizing hormone, were produce by baculovirus silkworm larvae system. We produced goldfish, Manchurian trout, and Japanese eel gonadotropins. Biological activities of goldfish and Manchurian trout gonadotropins were confirmed by in vivo and in vitro bioassays: Induction of sex steroid production, spermatogenesis, milt production, ovulation were confirmed. It is also confirmed that the yield of recombinant hormone by baculovirus silkworm larvae system is much higher than that of other systems previously reported, such as systems using yeast, amoeba, insect cells.Manchurian trout gonadotropins were purified and provided to other research groups for their research : two groups in Japan, and groups in USA, Netherlands, and Israel The results of present study suggest that our recombinant fish gonadotropins could be used for basic research of fish reproduction and also for induction of gonadal maturation in aquaculture fishes.