Production and provision of recombinant fish gonadotropins

重组鱼促性腺激素的生产和提供

• 批准号: 18580192
• 负责人: KOBAYASHI Makito
• 金额: $ 2.49万
• 依托单位: International Christian University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580192/
• 关键词: recombinant hormone; gonadotronin; luteinizing hormone; follicle-stimulating hormone; goldfish; Manchurian trout; eel; silkworm; 遺伝子組み換えホルモン; バキュロウィルス; マス

Recombinant fish gonadotropins, follicle-stimulating hormone and lutenizing hormone, were produce by baculovirus silkworm larvae system. We produced goldfish, Manchurian trout, and Japanese eel gonadotropins. Biological activities of goldfish and Manchurian trout gonadotropins were confirmed by in vivo and in vitro bioassays: Induction of sex steroid production, spermatogenesis, milt production, ovulation were confirmed. It is also confirmed that the yield of recombinant hormone by baculovirus silkworm larvae system is much higher than that of other systems previously reported, such as systems using yeast, amoeba, insect cells.Manchurian trout gonadotropins were purified and provided to other research groups for their research : two groups in Japan, and groups in USA, Netherlands, and Israel The results of present study suggest that our recombinant fish gonadotropins could be used for basic research of fish reproduction and also for induction of gonadal maturation in aquaculture fishes.

利用杆状病毒家蚕幼虫系统生产重组鱼促性腺激素促卵泡激素和促黄体生成激素。我们生产了金鱼、东北鳟鱼和日本鳗鱼的促性腺激素。通过体内和体外生物测定证实了金鱼和东北红点鲑促性腺激素的生物学活性：证实了其诱导性类固醇产生、精子发生、精液产生和排卵。同时也证实了杆状病毒家蚕幼虫系统的重组激素产量远高于以往报道的其他系统，如酵母、阿米巴、昆虫细胞系统。纯化了东北鳟鱼促性腺激素，并提供给其他研究组用于研究：日本的两个小组，美国，荷兰，本研究结果表明，我们的重组鱼促性腺激素可用于鱼类生殖的基础研究，也可用于诱导性腺成熟，水产养殖鱼类

项目成果

カイコ幼虫系による魚類組換え生殖腺刺激ホルモンの生産

蚕幼体系统生产鱼类重组促性腺激素

• 发表时间: 2008
• 作者: 小林 牧人;他
• 通讯作者: 他

Biological activities of single-chain recombinant goldfish follicle stimulating hormone and luteinizing hormone

单链重组金鱼卵泡刺激素和黄体生成素的生物活性

• 发表时间: 2007
• 作者: Hayakawa;et. al.
• 通讯作者: et. al.

Induction of spermatogenesis by recombinant goldfish gonadotropins in male Japanese eel

重组金鱼促性腺激素诱导雄性日本鳗鱼精子发生

• 发表时间: 2007
• 作者: Hayakawa;et. al.
• 通讯作者: et. al.

カイコでつくる魚類組換え生殖腺刺激ホルモン

蚕生产的重组鱼促性腺激素

• 发表时间: 2007
• 作者: 早川 洋一;他;小林 牧人
• 通讯作者: 小林 牧人

Biological activities of single-chain goldfish follicle-stimulating hormone and luteinizing hormone

• DOI: 10.1016/j.aquaculture.2007.12.002
• 发表时间: 2008-02-05
• 期刊: AQUACULTURE
• 影响因子: 4.5
• 作者: Hayakawa, Youichi;Morita, Tetsuro;Kobayashi, Makito
• 通讯作者: Kobayashi, Makito