Development of an vaccine commonly effective against different serotypes in fish pathogenic bacterium Edwardsiella tarda

开发针对鱼类病原菌迟缓爱德华氏菌不同血清型普遍有效的疫苗

基本信息

  • 批准号:
    14360111
  • 负责人:
  • 金额:
    $ 7.94万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2004
  • 项目状态:
    已结题

项目摘要

As there are many serotypes in fish pathogenic bacterium Edwardsiella tarda it has been considered difficult to produce effective vaccine against this disease. Present research project is intended to develop an.vaccine based on a commonly protective antigen among the different serotypes of E.tarda and obtained following results.A 37kDa outer membrane protein(OMP) was found as a common antigen among different serotypes of E.tarda. This protein was detected as separated one band by general SDS-PAGE and Western blotting. Two-dimensional electrophoresis and Western blotting showed the 37kDa OMP was detected as separate several dots with different PIs. However, these proteins showed similar antigenicity and same amino acid sequences, which indicate small difference in the molecular structure due to phosphonium or glycosyl modifications. N-terminal amino acid sequence of the protein and complete nucleotide sequence of the gene demonstrated that the 37kDa OMP is a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) found in a variety of bacteria. The recombinant GAPDH (rGAPDH) of E. tarda over expressed by using a Escherichia coli expression system was immunized to Japanese flounder and evaluated its vaccine efficacy. The results showed that the immunization with rGAPDH effectively protected Japanese flounder against infection with the different serotypic strains of E.tarda. The immune serum of these fish demonstrated increase in the antibody titer against those strains. The results show that the use of the rGAPDH will enable to develop a commonly effective vaccine against any serotypes of E.tarda.
由于鱼类病原性迟缓爱德华氏菌的血清型众多,因此很难研制出有效的疫苗。本研究以不同血清型迟缓艾美耳球虫的共同保护性抗原为基础,研制了迟缓艾美耳球虫疫苗,并获得了以下结果:发现迟缓艾美耳球虫不同血清型的共同保护性抗原是一种分子量为37 kDa的外膜蛋白(OMP)。该蛋白经常规SDS-PAGE和Western blotting检测为分离的一条带。双向电泳和Western blotting结果显示,37 kDa的OMP以多个点的形式存在,其PI值不同。然而,这些蛋白质显示出相似的抗原性和相同的氨基酸序列,这表明由于鳞或糖基修饰而导致的分子结构的微小差异。该蛋白的N末端氨基酸序列和该基因的完整核苷酸序列表明,37 kDa的OMP是一种在多种细菌中发现的甘油醛-3-磷酸脱氢酶(GAPDH)。重组E.利用大肠杆菌表达系统过表达的tarda蛋白免疫牙鲆,并对其免疫效果进行评价。结果表明,rGAPDH免疫能有效地保护牙鲆免受不同血清型迟缓艾美耳球虫的感染。这些鱼的免疫血清显示针对这些菌株的抗体滴度增加。结果表明,使用的rGAPDH将能够开发一个共同有效的疫苗对任何血清型的迟缓艾美耳球虫。

项目成果

期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Protective antigenicity of 37 kDa outer membrane protein of Edwardsiella tarda against different serotype strains
迟缓爱德华氏菌37 kDa外膜蛋白对不同血清型菌株的保护性抗原性
K.Kawai: "The point for development of bacterial fish vaccine"Proceedings of The JSPS-NRCT International Synposium, Perspective approaches for environmental and health management in aquaculture, Rayong, Thailand, 2002. 119-128 (2002)
K.Kawai:“细菌鱼疫苗的开发要点”JSPS-NRCT 国际研讨会论文集,水产养殖环境和健康管理的视角方法,泰国罗勇,2002 年。119-128 (2002)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kenji Kawai: "A conserved 37kDa outer membrane protein of Edwardsiella tarda is an effective vaccine candidate"Vaccine. (in press). (2004)
Kenji Kawai:“迟缓爱德华氏菌的一种保守的 37kDa 外膜蛋白是一种有效的候选疫苗”疫苗。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Vaccine efficacy of recombinant GAPDH of Edwardsiella tarda against edwardsiellosis
迟缓爱德华氏菌重组GAPDH抗爱德华氏菌病的疫苗效果
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KAWAI Kenji其他文献

KAWAI Kenji的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KAWAI Kenji', 18)}}的其他基金

Waveform inversion for localized 3-D structure in D"
D中局域三维结构波形反演"
  • 批准号:
    24840020
  • 财政年份:
    2012
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Protectivity of extensive infections by a bacterial outer membrane protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
细菌外膜蛋白甘油醛-3-磷酸脱氢酶 (GAPDH) 对广泛感染的保护作用
  • 批准号:
    23380111
  • 财政年份:
    2011
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
A study on a simple evaluation method for sulfuric acid resistivity of acid-resistant cementitious materials
耐酸胶凝材料耐硫酸性能简易评价方法的研究
  • 批准号:
    20360192
  • 财政年份:
    2008
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Durability Evaluation of Concrete Subject to the Attack of Corrosive Acidic Substances
混凝土受酸性腐蚀性物质侵蚀的耐久性评价
  • 批准号:
    18360209
  • 财政年份:
    2006
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development and Practicability Study on Methods for Assessing Environmental Impact of Concrete
混凝土环境影响评价方法的开发及实用性研究
  • 批准号:
    14350234
  • 财政年份:
    2002
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
CHLORIDE FIXATION CAPACITY OF CEMENT MANUFACTURED FROM MUNICIPAL SOLID WASTES
城市固体废物水泥的固氯能力
  • 批准号:
    12650461
  • 财政年份:
    2000
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on the vaccine against infection of Pathogenic bacterium Edwardsiella tarda
迟缓爱德华氏菌感染疫苗的研究
  • 批准号:
    11460090
  • 财政年份:
    1999
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Analysis of immune-reactive components of ayu serum using monoclonal antibody
使用单克隆抗体分析香鱼血清的免疫反应成分
  • 批准号:
    62560195
  • 财政年份:
    1987
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Exploring the periplasmic chaperone network underlying outer membrane protein folding
探索外膜蛋白折叠背后的周质伴侣网络
  • 批准号:
    22K05387
  • 财政年份:
    2022
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Unravelling the rules of outer membrane protein folding: Opportunities for the design of new antibacterial strategies
揭示外膜蛋白折叠规则:设计新抗菌策略的机会
  • 批准号:
    2602428
  • 财政年份:
    2021
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Studentship
Specific T and B cell responses to candidate Treponema pallidum outer membrane protein vaccine antigens
对候选梅毒螺旋体外膜蛋白疫苗抗原的特异性 T 和 B 细胞反应
  • 批准号:
    10671517
  • 财政年份:
    2019
  • 资助金额:
    $ 7.94万
  • 项目类别:
Specific T and B cell responses to candidate Treponema pallidum outer membrane protein vaccine antigens
对候选梅毒螺旋体外膜蛋白疫苗抗原的特异性 T 和 B 细胞反应
  • 批准号:
    10219125
  • 财政年份:
    2019
  • 资助金额:
    $ 7.94万
  • 项目类别:
Specific T and B cell responses to candidate Treponema pallidum outer membrane protein vaccine antigens
对候选梅毒螺旋体外膜蛋白疫苗抗原的特异性 T 和 B 细胞反应
  • 批准号:
    10461741
  • 财政年份:
    2019
  • 资助金额:
    $ 7.94万
  • 项目类别:
Specific T and B cell responses to candidate Treponema pallidum outer membrane protein vaccine antigens
对候选梅毒螺旋体外膜蛋白疫苗抗原的特异性 T 和 B 细胞反应
  • 批准号:
    9982776
  • 财政年份:
    2019
  • 资助金额:
    $ 7.94万
  • 项目类别:
Role of outer membrane protein methylation in the process of bacterial environmental adaptation
外膜蛋白甲基化在细菌环境适应过程中的作用
  • 批准号:
    327196-2012
  • 财政年份:
    2018
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Discovery Grants Program - Individual
Dynamic characterization of outer-membrane-protein biogenesis by the BAM and TAM complexes
BAM 和 TAM 复合物外膜蛋白生物合成的动态表征
  • 批准号:
    10204038
  • 财政年份:
    2017
  • 资助金额:
    $ 7.94万
  • 项目类别:
Dynamic characterization of outer-membrane-protein biogenesis by the BAM and TAM complexes
BAM 和 TAM 复合物外膜蛋白生物合成的动态表征
  • 批准号:
    9398209
  • 财政年份:
    2017
  • 资助金额:
    $ 7.94万
  • 项目类别:
Investigation of outer membrane protein recognition mechniams by human pathgen recognition receptors
人类病原体识别受体外膜蛋白识别机制的研究
  • 批准号:
    16K18506
  • 财政年份:
    2016
  • 资助金额:
    $ 7.94万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了