Study of 14-3-3 sigma protein in normal and neoplastic tissues.

正常组织和肿瘤组织中 14-3-3 σ 蛋白的研究。

• 批准号: 14370066
• 负责人: NAKAJIMA Takashi
• 金额: $ 3.01万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370066/
• 关键词: 14-3-3 sigma protein; Epithelial cells; Lung cancer; Breast cancer; Colon cancer; Immunohistochemistry; DNA methylation; 正常組織; 腫瘍組織; 扁平上皮; 基底細胞; 筋上皮細胞; 偏平上皮癌

In normal human tissues, 14-3-3 σ protein was exclusively present in various epithelial cells, of which squamous epithelia at various sites showed the strongest immunoreactivity and basal cells of respiratory tract or myoepithelial cells of various organs followed. In non-small cell carcinoma of the lung, squamous cell carcinoma expressed stronger immunoreactivity for 14-3-3 σ protein than adenocarcinoma. In human colon cancers, immunoreactivity for 14-3-3 σ protein varied from area to area or case to case. However, there was a tendency to be positive for 14-3-3 σ expression at tumor invasion front. Molecular biological study revealed that DNA methylation of the gene did not influence the expression of the 14-3-3 σ gene in colon cancers. In breast carcinogenesis, down-regulation of 14-3-3 σ protein was well correlated with its progression. In ductal carcinoma lesions, even in non-invasive, marked down-regulation of 14-3-3 σ expression was observed. Breast borderline lesion, columnar cell hyperplasia with atypia showed different 14-3-3 σ expression pattern from that of ductal cancer lesion, suggesting it to be precancerous lesion of the breast.

在正常人体组织中，14-3-3 σ蛋白仅存在于各种上皮细胞中，其中各部位的鳞状上皮细胞免疫反应性最强，其次是呼吸道基底细胞或各器官的肌上皮细胞。在非小细胞肺癌中，鳞癌中14-3-3 σ蛋白的表达强于腺癌。在人结肠癌中，14-3-3 σ蛋白的免疫反应性因地区或病例而异。而14-3-3 σ在肿瘤浸润前沿呈阳性表达。分子生物学研究表明，14-3-3 σ基因在结肠癌中的表达不受DNA甲基化的影响。在乳腺癌发生过程中，14-3-3 σ蛋白表达下调与乳腺癌的进展密切相关。在导管癌病变中，甚至在非侵袭性中，观察到14-3-3 σ表达的显著下调。乳腺交界性病变、柱状细胞增生伴乳头状瘤的14-3-3 σ表达模式与乳腺导管癌不同，提示其为乳腺癌前病变。

项目成果

Takashi Nakajima, Hanako Shimooka, Peng Weixa, Atsuki Segawa, Atsushi Motegi, Zhang Jian, Norihiko Masuda, Munenori Ide, Takaaki Sano, Tetsunari Oyama, Hiroe Tsukagoshi, Kozue Hamanaka, Masahiro Maeda: "Immunohistochemical demonstration of 14-3-3 sigma pr

Takashi Nakajima、Hanako Shimooka、Peng Weixa、Atsuki Sekawa、Atsushi Motegi、张健、Norihiko Masuda、Munenori Ide、Takaaki Sano、Tetsunari Oyama、Hiroe Tsukagoshi、Kozue Hamanaka、Masahiro Maeda：“14-3-3 sigma pr 的免疫组织化学演示

Nakajima, T. et al.: "Immunohistochemical demonstration of 14-3-3 sigma protein in normal human tissues and lung cancers, and the preponderance of its strong expression in epithelial cells of squamous cell lineage"Pathology International. (in press). (200

Nakajima, T. 等人：“正常人体组织和肺癌中 14-3-3 σ 蛋白的免疫组织化学证明，以及其在鳞状细胞谱系上皮细胞中强表达的优势”《国际病理学》。

Hanako Simooka, Tetsunari Oyama, Takaaki Sano, Jun Horiguchi, Takashi Nakajima: "Immunohistochemical analysis of 14-3-3 sigma and related proteins in hyperplastic and neoplastic breast lesions, with particular reference to early carcinogenesis."Pathology

Hanako Simooka、Tetsunari Oyama、Takaaki Sano、Jun Horiguchi、Takashi Nakajima：“对增生性和肿瘤性乳腺病变中 14-3-3 sigma 和相关蛋白进行免疫组织化学分析，特别是早期癌变。”病理学

Munenori Ide, Takashi Nakajima, Takayuki Asao, Hiroyuki Kuwano: "Inactivation of 14-3-3σ by hypermethylation is a rare event in colorectal cancers and its expression may correlate with cell cycle maintenance at the invasion front."Cancer Letter. (In press

Munenori Ide、Takashi Nakajima、Takayuki Asao、Hiroyuki Kuwano：“14-3-3σ 因高甲基化而失活在结直肠癌中是罕见的事件，其表达可能与侵袭前沿的细胞周期维持相关。”

Hanako Simooka, Tetsunari Oyama, Takaaki Sano, Jun Horiguchi, Takashi Nakajima: "Immunohistochemical analysis of 14-3-3 sigma and related proteins in hyperplastic and neoplastic breast lesions, with particular reference to early carcinogenesis."Pathol Int

Hanako Simooka、Tetsunari Oyama、Takaaki Sano、Jun Horiguchi、Takashi Nakajima：“对增生性和肿瘤性乳腺病变中 14-3-3 sigma 和相关蛋白进行免疫组织化学分析，特别是早期癌变。”Pathol Int