The pathogenesis of Type 1 diabetes in children-the role of endogenous retrovirus infection in relation with disposition, environment and auto-immunity

在这两年中，我们进行了以下研究：1)新诊酮症1型糖尿病患儿肠道病毒m RNA的提取：95例新诊1型糖尿病患者血清和26例单核细胞标本中提取肠道病毒m RNA(VP4)，但无一例血清和单核细胞分离不出病毒m RNA。2)我们在IDDMK_(1.2)&gt；22超抗原编码区的290和461位核苷酸位置发现了两个非同义的A/G多态。Conrad B博士对超抗原活性和这种多态性感兴趣，并要求我们与1型糖尿病儿童合作。然而，由于种种原因，这些合作研究最终没有取得成功。3)儿童缓慢进展型糖尿病的临床和遗传学特点：通过对儿童急性起病1型糖尿病、慢进型糖尿病、非进展型糖尿病和正常对照组的HLADQ-β，-α基因、DR-β基因的分析，探讨慢进型糖尿病的发病机制。我们发现SPIDDM的发病年龄比急性起病大得多，而胰岛抗体的阳性率几乎相同。人类白细胞抗原基因与NIDDM和正常对照组不同。DRB1 0405-DQB1 0401很常见，可能是1型糖尿病的一个亚型。

We have studied the following projects during these two years 1) Isolation of Enterovirus m RNA from newly diagnosis of ketosis-phone type 1 diabetic children : 95 serum and 26 mononuclear cells samples from newly diagnosis type 1 diabetes were tried to extract entero-virus mRNA(VP4), however non of the serum samples nor mononuclear cells were failed to isolate virus mRNA. 2) We identified two nonsynonymous A/G polymorphisms in the superantigen-coding region of IDDMK_<1.2>22 at the 290-and 461-nucleotide position from the initial methione coden. Dr.Conrad B was interested in relation to super -antigen activity and this polymorphism and asked us to collaborate with type 1 diabetic children. However, in some reason, these collaborate studies were finally not succeeded due to their reasons. 3) Clinical and genetic characteristic of slowly progressing type 1 diabetes(SPIDDM) in children : We have analyzed HLA DQ-β, -αgene, DR-βgene, in children with acute-onset type 1 diabetes, SPIDDM, NIDDM and normal controls to identified the pathogenesis of SPIDDM. We found that age at onset of SPIDDM is much elder than acute onset and positivity of islet antibodies was almost same. HLA genes were different from NIDDM and controls. DRB1 0405-DQB1 0401 is popular and will be a subtype of type 1 diabetes mellitus.