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Pathogenesis and long-team prognosis of childhood onset insulin-dependent diabetes mellitus (IDDM)

儿童期胰岛素依赖型糖尿病（IDDM）的发病机制和长期预后

基本信息

批准号：
07457182
负责人：
MATSUURA Nobuo
金额：
$ 3.84万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457182/
关键词：
HLA antigen genes DR beta genes Mitochondrial genes GAD antigen DT diaphorase Cox B virus Autoantigens ICA512 HLA抗原 IDDM(インスリン依存性糖尿病)ミトコンドリヤ遺伝子異常 ICA512(TA-Z)コクサッキーBウイルス RNA DQ抗原遺伝子 DR抗原遺伝子 DQβ鎖遺伝子 DQα鎖遺伝子 diabetogenic

项目摘要

1.Detection of Coxsackie B virus RNA in serum from children at the onset of IDDMTo elucidate the environmental factors of childhood IDDM,children at onset of IDDM were investigated by detection of specific Cox B sequences in serum using a transcription and a nested polymerase chain reaction (nested RT-PCR). We studied 59 cases of children with IDDM at onset, however we can't detected none of the cases until now. We are continuing to try to isolate Cox B specific virus RNA.2.HLA-DR,DQ beta genes and DQ alpha genes analysisWe analyzed HLA antigen genes in 104 patients with IDDM and 97 controls. We found significantly decreased frequency in DRB1 150X (D), and increased in 0405 (S), 0901 (V). Moreover, 0802(D) was significantly in IDDM.DQA1 (R), DQB1 (D), DRB1 (nD) homozygotes was found most susceptibility genotype (RR=2.87) in Japanese IDDM patients.3.Mitochondrial (Mt) gene mutations associated with Japanese childhood IDDM pat ients. : We found none of the patients had 3243 (A-G) mutations, however, 2 and 5 of the 155 IDDM patients had 3316 and 3394 mutations.4.Long-term complications of IDDM patients and interational collaboration studies : We collaborated in DERI,DIAMOND and Hvidore study groups.

1. IDDM患儿血清中科萨基B病毒RNA的检测为探讨儿童期IDDM的环境因素，采用转录和巢式聚合酶链反应（nested RT-PCR）检测IDDM患儿血清中特异性考克斯B序列。我们研究了59例儿童原发性胰岛素依赖型糖尿病（IDDM），但至今未能发现一例。2.HLA-DR、DQ β和DQ α基因分析对104例IDDM患者和97例对照者进行了HLA抗原基因分析。DRB 1 150 X（D）的频率显著降低，而0405（S）、0901（V）的频率显著升高。DQA 1（R）、DQB 1（D）、DRB 1（nD）纯合子是日本人IDDM的最易患基因型（RR=2.87）。3.线粒体（Mt）基因突变与日本儿童IDDM相关。结果：155例IDDM患者中无一例存在3243（A-G）突变，而3316和3394突变分别为2例和5例。4. IDDM患者远期并发症及国际协作研究：DERI、DIAMOND和Hviolet三个研究组进行了协作研究。