Pathogenesis and long-team prognosis of childhood onset insulin-dependent diabetes mellitus (IDDM)

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1.Detection of Coxsackie B virus RNA in serum from children at the onset of IDDMTo elucidate the environmental factors of childhood IDDM,children at onset of IDDM were investigated by detection of specific Cox B sequences in serum using a transcription and a nested polymerase chain reaction (nested RT-PCR). We studied 59 cases of children with IDDM at onset, however we can't detected none of the cases until now. We are continuing to try to isolate Cox B specific virus RNA.2.HLA-DR,DQ beta genes and DQ alpha genes analysisWe analyzed HLA antigen genes in 104 patients with IDDM and 97 controls. We found significantly decreased frequency in DRB1 150X (D), and increased in 0405 (S), 0901 (V). Moreover, 0802(D) was significantly in IDDM.DQA1 (R), DQB1 (D), DRB1 (nD) homozygotes was found most susceptibility genotype (RR=2.87) in Japanese IDDM patients.3.Mitochondrial (Mt) gene mutations associated with Japanese childhood IDDM pat ients. : We found none of the patients had 3243 (A-G) mutations, however, 2 and 5 of the 155 IDDM patients had 3316 and 3394 mutations.4.Long-term complications of IDDM patients and interational collaboration studies : We collaborated in DERI,DIAMOND and Hvidore study groups.