Construction of therapeutic gene library for pancreatic cancer and development of specific therapeutic vector

胰腺癌治疗基因库的构建及特异性治疗载体的开发

• 批准号: 14370345
• 负责人: KATOH Hiroyuki
• 金额: $ 9.02万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370345/
• 关键词: pancreatic cancer diagnosis; gene therapy; cDNA library; RCAS1; caveolin-A; PEDF; PEDF; 膵臓; 遺伝子ライブラリー; アデノウイルスベクター

We constructed a frozen tissue library containing cancer tissues obtained from 5,000 patients who agreed with informed consent and underwent surgical treatment at Hokkaido University Hospital and its 33 affiliated hospitals. We had performed gene expression assay of 31 patients with pancreatic adenocarcinoma till December in 2003, and made the expression profile of 1289 genes correlated with cancer progression. On the other hand, we reported that several gene expression such as caveolin-1, RCAS1, and PEDF correlate with prognosis of patients with pancreatic cancer. We thought that PEDF gene therapy is potentially useful for the treatment of pancreatic cancer patients with liver metastasis.

我们构建了一个冷冻组织库，其中包含从北海道大学医院及其33家附属医院接受手术治疗的5，000名患者中获得的癌症组织。2003年12月，我们对31例胰腺癌患者进行了基因表达检测，获得了1289个与胰腺癌进展相关的基因表达谱。另一方面，我们报道了一些基因表达，如小窝蛋白-1，RCAS 1和PEDF与胰腺癌患者的预后相关。我们认为PEDF基因治疗对胰腺癌肝转移患者有潜在的治疗价值。

项目成果

Hiraoka K: "High expression of tumor-associated antigen RCAS1 in pancreatic ductal adenocarcinoma is an unfavorable prognostic marker"Int J Cancer. 99. 418-423 (2002)

Hiraoka K：“胰腺导管腺癌中肿瘤相关抗原 RCAS1 的高表达是一个不利的预后标志物”Int J Cancer。

Uehara H: "Expression of Pigment Epithelium-derived Factor Decreases Liver Metastasis and Correlates with Favorable Prognosis for Patients with Ductal Pancreatic Adenocarcinoma"Cancer Res. (In Press).

Uehara H：“色素上皮衍生因子的表达可减少肝转移，并与导管胰腺腺癌患者的良好预后相关”Cancer Res。

Hideaki Hashida: "Fusion of Tat protein transduction domain to poly-lysine as a new DNA delivery tool."British Journal of Cancer. 90. 1252-1258 (2004)

Hideaki Hashida：“Tat 蛋白转导结构域与聚赖氨酸的融合作为新的 DNA 传递工具。”英国癌症杂志。

Hashida H: "Fusion of HIV-1 Tat protein transduction domain to poly-lysine as a new DNA delivery tool"Br J Cancer. 90. 1252-1258 (2004)

Hashida H：“HIV-1 Tat 蛋白转导结构域与聚赖氨酸的融合作为新的 DNA 传递工具”Br J Cancer。

Suzuoki M: "Impact of caveolin-1 expression on prognosis of pancreatic ductal adenocarcinoma"Br J Cancer. 87. 1140-1144 (2002)

Suzuoki M：“caveolin-1 表达对胰腺导管腺癌预后的影响”Br J Cancer。