Developing a gene therapy product to treat pressure ulcers in lower-limb amputees

开发一种基因治疗产品来治疗下肢截肢者的压力性溃疡

• 批准号: 2888189
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2888189
• 关键词: Developing; gene; therapy; product; treat

One of the many functions of the skin is to provide a protective barrier and resist mechanical loads as we interact with our environment. Sustained mechanical loads can cause skin injurysuch as pressure ulcers, which are painful, difficult and costly to treat, and reduce patients' quality of life. As many as 26% of lower-limb prosthesis users suffer from pressure ulcers at the interface with the device. This occurs because the skin on the residual limb is not adapted to tolerate the loads necessary for ambulation. This is in contrast to plantar skin on the sole of the foot, whose thick stratum corneum and expression of keratin 9 enhance its load tolerance. Epidermal compositions develop differently because of positional information from fibroblasts within the skin dermis. A novel strategy has been proposed wherein stump skin can be reprogrammed to act as load-bearing plantar skin by transplanting autologous plantar fibroblasts to the residual limb. This research project aims to bypass the need for autologous cells, and instead directly use the genes that control plantar fate to endow stump skin with load-bearing abilities

皮肤的众多功能之一是在与环境相互作用时提供保护性屏障并抵抗机械载荷。持续的机械负荷会导致皮肤损伤，因为压力性溃疡，痛苦，困难和昂贵的治疗和降低患者的生活质量。多达26％的下LIMB假体使用者在与设备的接口处遭受压力溃疡。之所以发生这种情况，是因为残留肢体上的皮肤不适合容忍行走所需的负载。这与脚鞋底上的足底皮肤相反，脚的底部厚地层和角蛋白9的表达增强了其载荷的耐受性。由于皮肤真皮内的成纤维细胞的位置信息，表皮成分的发展不同。已经提出了一种新型策略，可以将树桩皮肤重新编程为通过将自体足底成纤维细胞移植到残留的肢体中，以充当承重的底型皮肤。该研究项目旨在绕过对自体细胞的需求，而直接使用控制足底命运的基因来赋予皮肤具有承重能力