Developing a gene therapy product to treat pressure ulcers in lower-limb amputees

开发一种基因治疗产品来治疗下肢截肢者的压力性溃疡

• 批准号: 2888189
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2888189
• 关键词: Developing; gene; therapy; product; treat

One of the many functions of the skin is to provide a protective barrier and resist mechanical loads as we interact with our environment. Sustained mechanical loads can cause skin injurysuch as pressure ulcers, which are painful, difficult and costly to treat, and reduce patients' quality of life. As many as 26% of lower-limb prosthesis users suffer from pressure ulcers at the interface with the device. This occurs because the skin on the residual limb is not adapted to tolerate the loads necessary for ambulation. This is in contrast to plantar skin on the sole of the foot, whose thick stratum corneum and expression of keratin 9 enhance its load tolerance. Epidermal compositions develop differently because of positional information from fibroblasts within the skin dermis. A novel strategy has been proposed wherein stump skin can be reprogrammed to act as load-bearing plantar skin by transplanting autologous plantar fibroblasts to the residual limb. This research project aims to bypass the need for autologous cells, and instead directly use the genes that control plantar fate to endow stump skin with load-bearing abilities

皮肤的许多功能之一是提供保护屏障，并在我们与环境相互作用时抵抗机械负荷。持续的机械载荷可能会导致皮肤损伤，例如压疮，这种损伤会带来疼痛、困难且治疗成本高昂，并降低患者的生活质量。多达26%的下肢假肢使用者在与设备的接口处患有压疮。这是因为残肢上的皮肤不适于承受截肢所需的负荷。这与脚底上的足底皮肤形成对比，足底皮肤的厚角质层和角蛋白9的表达增强了其负荷耐受性。由于来自皮肤真皮内的成纤维细胞的位置信息，表皮组合物发育不同。已经提出了一种新的策略，其中残端皮肤可以通过将自体足底成纤维细胞移植到残肢来重新编程以充当承重足底皮肤。这项研究项目旨在绕过对自体细胞的需求，而是直接使用控制足底命运的基因来赋予残肢皮肤承重能力