Development of specific vector containing FN-1 fused protein and SCCA promoter for esophageal cancer

为了寻找食管鳞状细胞癌（ESCC）的特异性基因表达，开发治疗基因，我们对食管鳞状细胞癌（ESCC）中的几种基因表达进行了筛选。然后我们发现RCAS 1和caveolin-1对于食道癌的靶点可能有用。随后，我们构建了一个穿梭载体，使腺病毒载体中引入SCCA 2启动子。构建了由SCCA 2启动子驱动的表达促凋亡基因KLAKLA 2的腺病毒载体。Ad-SCCAp-KLAKLAK在产生SCCA 2的细胞系LK 2和LC 1中诱导凋亡，但在不产生SCCA 2的A549或ABC-1中不诱导凋亡。用Ad-SCCA_p-KLAKLAK在裸鼠皮下移植瘤中观察了Ad-SCCA_p-KLAKLAK的基因治疗效果。

We screened several gene expressions in esophageal squamous cell carcinoma (ESCC) for the purpose of finding of cancer specific gene expression and development of therapeutic gene. Then we found that RCAS1 and caveolin-1 are potentially useful for the target of esophageal cancer. Subsequently, we constructed a shuttle vector to make adenoviral vectors in which SCCA2 promoter was introduced. An adenoviral vector which expresses proapoptotic gene KLAKLA2 driven by SCCA2 promoter was reconstructed. Ad-SCCAp-KLAKLAK induced apoptosis in SCCA2 producing cell lines LK2 and LC1, but not A549 or ABC-1 that did not produce SCCA2. Effects of gene therapy using Ad-SCCAp-KLAKLAK were observed in vivo SCCA2 producing tumors implanted on the hack of nude mice were successfully suppressed by Ad-SCCAp-KLAKLAK.