An artificial lung of a new concept, development of hemoglobin with the high oxygen affinity

新概念人工肺，开发高氧亲和力血红蛋白

• 批准号: 14370398
• 负责人: HOSHIKAWA Yasushi
• 金额: $ 8.77万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370398/
• 关键词: Human β-globin gene; Transgenic mice; Fetal hemoglobin; γ-globin gene; Assessment of physical activity by running-wheel apparatus; Short-chain fatty acid derivatives; α Methyl hydrocinnamic acid(AMHCA); ヒトβglobin遺伝子; トランスジュニックマウス; α methyl hydroc

1.Patients with chronic respiratory failure did not have fetal hemoglobin in their blood.2.Human β-globin transgenic (Tg) mice were imported from University of Kansas Medical Center and bred. Fetal hemoglobin was induced in these mice pharmacologically by Pace's method, that is treating the mice with α methyl hydrocinnamic acid (AMHCA), a short-chain fatty acid derivative, for 5 days. RT-PCR revealed that γ-globin gene was expressed in peripheral blood of the AMHCA-treated Tg mice.3.The Tg mice with the induction of γ-globin gene tended to show lower physical activity, assessed by the running-wheel apparatus, under hypoxic conditions when compared with their littermates without γ-globin gene induction.In conclusion, induction of fetal hemoglobin in mice does not improve physical activity under hypoxic conditions.

1. 2.从堪萨斯大学医学中心引进人β-珠蛋白转基因（Tg）小鼠并进行饲养。通过Pace方法在这些小鼠中诱导胎儿血红蛋白，即用α甲基氢化肉桂酸（AMHCA），一种短链脂肪酸衍生物处理小鼠5天。RT-PCR结果显示，经AMHCA处理的Tg小鼠外周血中有γ-珠蛋白基因的表达。3.转基因小鼠在低氧条件下的运动能力较同窝未转基因小鼠低。

项目成果

Peroxisome proliferator-activated receptor(PPAR)γ ligandsのラットモノクロタリン肺高血圧抑制効果

过氧化物酶体增殖物激活受体(PPAR)γ配体对野百合碱大鼠肺动脉高压的影响

• 期刊: 日本呼吸器学会雑誌 (印刷中)
• 作者: 松田安史;星川 康;ほか
• 通讯作者: ほか