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Therapeutic effect of Frzb on joint diseases

Frzb对关节疾病的治疗作用

基本信息

批准号：
14370472
负责人：
HORII Motoyuki
金额：
$ 8.58万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

The aim of this study is to investigate the therapeutic effect of Frzb on joint diseases and the mechanism of chondrocyte differentiation and apoptosis in their pathogenesis. Firstly, to elucidate the mechanism of chondrocyte apoptosis in osteoarthritis (OA), we examined the function of heat shock protein 70 (FISP 70), which was known to play a pivotal role in OA pathogenesis. HSP 70 was genetically transduced into chondrocytes by means of the adenovirus vector, and the treatment prohibited NO-or staurosporine-induced apoptosis. These findings help elucidate part of the pathogenesis of OA. Secondly, to demonstrate the therapeutic effect of Frzb on joint diseases, we investigate the influence of overexpressed Frzb on the cytokine expression by syonovial fibroblasts of rheumatoid arthritis (RA) and osteoblasts from subchondral bone of OA. Frzb was overexpressed into each primary cells by means of lipofection method. Total RNA was extracted and analyzed by RT-PCR method for IL-1β, IL-6,IL-8 and TNF-αmRNA expression. In osteoblasts from subchondral bone of OA, endogenous Frzb expression was detectable and the expression of inflammatory cytokines decreased after Frzb overexpression. In syonovial fibroblasts of RA, remarkable change of cytokine expression was not observed. These findings suggest that aberrant differentiation of subchondral bone can be involved in the pathogenesis of OA and Frzb can be therapeutic candidate gene of OA. Further research is necessary to find the relationship between Frzb and HSP 70 from the aspect of chondrocyte apoptosis and clarify in vivo therapeutic effect of Frzb on experimental OA in the rabbit knee.

本研究的目的是探讨Frzb对关节疾病的治疗作用以及软骨细胞分化和凋亡在其发病机制中的作用。首先，为了阐明骨关节炎（OA）中软骨细胞凋亡的机制，我们研究了热休克蛋白70（FISP 70）的功能，它在OA发病机制中起着关键作用。通过腺病毒载体将HSP 70基因转导到软骨细胞中，并且治疗抑制NO或星形孢菌素诱导的凋亡。这些发现有助于阐明OA的部分发病机制。其次，为了证明Frzb对关节疾病的治疗作用，我们研究了过表达的Frzb对类风湿性关节炎（RA）滑膜成纤维细胞和OA软骨下骨成骨细胞的细胞因子表达的影响。利用脂质体转染法将Frzb过表达于各原代细胞中。提取总RNA，RT-PCR检测IL-1β、IL-6、IL-8和TNF-αmRNA的表达。在OA的软骨下骨的成骨细胞中，内源性Frzb表达可检测到，并且Frzb过表达后炎性细胞因子的表达减少。RA滑膜成纤维细胞中细胞因子表达无明显变化。这些结果提示软骨下骨的异常分化可能参与了OA的发病过程，Frzb基因可能是OA的治疗候选基因。从软骨细胞凋亡的角度探讨Frzb与HSP 70的关系，阐明Frzb对实验性兔膝关节OA的体内治疗作用，有待于进一步研究。