Role of Rho-kinase in cerebral vasospasm and effect of inhalational anesthetics

Rho激酶在脑血管痉挛中的作用及吸入麻醉药的作用

• 批准号: 14370482
• 负责人: FUJIHARA Hideyoshi
• 金额: $ 7.81万
• 依托单位: UNIVERSITY OF FUKUI (2004)福井医科大学 (2002-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370482/
• 关键词: Rho-kinase; protein kinase C; intracellular calcium

To study the role of PKC in the mechanism of contraction in cerebral artery, using porcine cerebral artery, following experiments were planned :1)The eleven PKC isofomis were explored by Western blotting method following SDS-PAGE.2)It is known that one of small GTP binding protein, RhoA, and its downstream effecor, Rho-kinase (Rock II) participate in the calcium sensitization. The presence of these proteins, distribution were examined by Western blotting.3)Myosin light chain (MLC) and phosphorylated myosin light chain (p-MLC) were subjected to Western Blotting.4)Two dimentional electrophoresis, silver staining were applied to detect and quantify the p-MLC/MLC ratio.The presence of the mechasnism of calcium sensitization through Rho-Rho-kinase pathway was indicated.The other mechanism of calcium sensitization, the PKC -mediated pathway was also indicated due to the presence of the conventional PKCs, α, βI, βII.

为了研究PKC在脑动脉收缩机制中的作用，以猪脑动脉为实验材料，计划进行以下实验：1）采用SDS-PAGE后的Western blotting方法对11种PKC亚型进行了研究。2）已知小GTP结合蛋白之一RhoA及其下游效应物Rho-激酶（Rock II）参与钙致敏作用。用蛋白质印迹法检测肌球蛋白轻链（MLC）和磷酸化肌球蛋白轻链（p-MLC）的蛋白质分布;用双向电泳、银染等方法检测p-MLC/MLC的比值，并对p-MLC/MLC的比值进行定量分析。由于存在常规的PKC α、βI、βII，也表明PKC介导的途径。

项目成果

Yoshikazu Yasuda: "Orexin A elicits arousal electroencephalography without sympathetic cardiovascular activation in isoflurane -anesthetized rats"Anesthesia and Analgesia. 97・6. 1663-1666 (2003)

Yoshikazu Yasuda：“Orexin A 在异氟烷麻醉的大鼠中引发唤醒脑电图，而不激活交感神经心血管”麻醉和镇痛 1663-1666。

Ippei Watanabe: "Beneficial effect of a prone position for patients with hypoxemia after transthoracic esophagectomy"Critical Care Medicine. 30・8. 1799-1802 (2002)

渡边一平：“俯卧位对经胸食管切除术后低氧血症患者的有益作用”《重症监护医学》30・8（2002）。

オレキシン

食欲素

• 发表时间: 2004
• 期刊: 臨床麻酔 28・8
• 作者: 鈴木久人;安田善一;江口広毅他12名
• 通讯作者: 江口広毅他12名

麻酔薬及び麻酔関連薬使用ガイドライン(改訂第2版)

麻醉药及麻醉相关药品使用指南（修订第2版）

• 发表时间: 2004
• 作者: 冨士原 秀善(分担執筆)

The effect of intraventricular application of Orexin A on electroencephalography and sympathetic nervous activity in isoflurane -anesthetized rats.

脑室内应用 Orexin A 对异氟烷麻醉大鼠脑电图和交感神经活动的影响。

• 发表时间: 2004
• 期刊: Neuroanesthesia and intensive care 2003
• 作者: Yasuda;Y.
• 通讯作者: Y.