Reconstruction of the urinary tract with biological scaffold combined with sustained release of growth factor.

1)膀胱脱细胞基质与外源性生长因子缓释复合的膀胱再生（参考文献1）。当含有bFGF的BAM在大鼠膀胱增大模型中用作修复移植物时，bFGF的掺入能促进血管生成，抑制移植物的收缩，并呈剂量依赖性; 2）胶原基质作为外源性生长因子的释放载体BAM释放各种生长因子的性质与纯I型胶原海绵的性质相似。I型胶原蛋白可作为碱性成纤维细胞生长因子的储存库（参考文献3）bFGF在体外和体内生理条件下与I型胶原蛋白溶液和海绵相互作用，并受到胶原蛋白的保护而免受蛋白水解环境的影响。4）在膀胱无细胞基质上的骨髓来源的细胞中诱导平滑肌细胞样表型（参考文献4）在膀胱修复模型中，骨髓来源的细胞重建无细胞移植物上的平滑肌层，天然平滑肌细胞（SMC）浸润移植物。在体外，SMC的生长因子环境特征诱导骨髓细胞中的SMC样表型，而尿路上皮cells failed.In总结，我们已经创建了一个新的释放系统从动物来源的extracellualr基质biomaterials，并证明了一个未被认识到的作用，I型胶原控制生长因子的功能。参考文献3证明了该原理用于治疗缺血性病症的临床适用性。同时，我们研究了生长因子对平滑肌再生的作用，并在参考文献1和4中提出了一种管理膀胱再生的创新机制。

1)Bladder regeneration by bladder acellular matrix combined with sustained release of exogenous growth factor (Reference 1).The bladder acellular matrix (BAM) functioned as release carrier of bFGF by itself. When the BAM containing bFGF was used as repair graft in a rat bladder augmentation model, angiogenesis into the matrix was promoted and graft shrinkage was significantly inhibited by incorporated bFGF in a dose dependent manner.2)Collagenous matrices as release carriers of exogenous growth factors (Reference 2).The property of BAM for the release of various growth factors was similar to that of pure Type I collagen sponge.3)Type I collagen can function as a reservoir of basic fibroblast growth factor (Reference 3).bFGF interacts with type I collagen solution and sponges under in vitro and in vivo physiological conditions, and is protected from the proteolytic environment by the collagen. This property could be successfully applied for treatment of severe ischemic limb.4)Induction of smooth muscle cell-like phenotype in marrow-derived cells on bladder acellular matrix (Reference 4)In bladder repair model, bone marrow-derived cells reconstituted the smooth muscle layer on acellular graft, with native smooth muscle cells (SMC) infiltrating the graft. In vitro, growth factor milieu characteristic of SMC induced an SMC-like phenotype in marrow cells, whereas that of urothelial cells failed.In summary, we have created a novel release system from animal-derived extracellualr matrix biomaterial, and demonstrated an unrecognized role of Type I collagen for controlling the function of growth factors. Reference 3 demonstrates a clinical applicability of this principle for treating ischemic conditions. In parallel, we have investigated roles of growth factors for smooth muscle regeneration, and presented an innovative mechanism that governs the bladder regeneration in References 1 and 4.