Study of gentic and epigenetic alterations in prostatic cancer using DNA microarray method.

使用 DNA 微阵列方法研究前列腺癌的遗传和表观遗传改变。

• 批准号: 14370513
• 负责人: NAITO Katsusuke
• 金额: $ 6.27万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370513/
• 关键词: prostate cancer; primary structure of genom; array CGH; ZD1839; PC-3; LNCap; DU-145; signal transduction; Arry CGH; LNCaP; ラテント癌; ゲノム一次構造異常; array CGH; PC3

Incidence of prostate cancer in Japan and resultant mortality rates are lower than in Western countries. To elucidate the reasons behind this, the genetic characteristics of prostate cancer in Japanese patients were investigated. Comparative genomic hybridization was applied in 27 cases of prostate cancers in Japanese patients. The frequency of 13q loss may imply differences in biological behavior of prostate cancer between Japan and Western coutries. To clarify a array comparative genomic hybridization(CGH) technique, we applied array CGH spotted with 283 specific genes to 11 clinical prostate cancers, and the results were compared with comparative genomic hybridization (conventional CGH) and loss of heterozygosity (LOH) analysis using miciosatellite DNA markers. When the results of both CGH techniques were compared with those of LOH analysis, the correspondence rate of array CGH was significantly higher than that of conventional CGH (93.4% vs. 72.2%, p<0.05).We examined the antiproliferative effects and mechanism of gefitinib (Iressa, ZD1839), an epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) on three human prostate cancer cell lines, LNCap (hormon-dependent growth), PC-3 (hormone-independent growth) and DU-145. A significant dose-dependent growth-inhibitory effect was observed in the gefinitib treatment group for each cell lines. cDNA microarray experiments showed that several genes were down regulated.

日本前列腺癌的发病率和死亡率低于西方国家。为了阐明这背后的原因，研究了日本患者前列腺癌的遗传特征。比较基因组杂交应用于27例日本前列腺癌患者。13 q丢失的频率可能意味着日本和西方国家前列腺癌生物学行为的差异。为了阐明阵列比较基因组杂交（CGH）技术，我们将283个特异性基因点样的阵列CGH应用于11例临床前列腺癌，并将结果与比较基因组杂交（常规CGH）和微卫星DNA标记的杂合性丢失（洛）分析进行比较。两种CGH技术与洛性分析结果比较，阵列CGH的符合率明显高于常规CGH（93.4%vs.72.2%，p<0.05）。我们研究了吉非替尼的抗增殖作用及其机制（Iressa，ZD 1839），一种表皮生长因子受体酪氨酸激酶抑制剂在三种人前列腺癌细胞系LNCap（EGFR依赖性生长）、PC-3（EGFR非依赖性生长）和DU-145上进行EGFR-TKI。在吉非替尼处理组中观察到每种细胞系的显著剂量依赖性生长抑制作用。cDNA微阵列实验表明，几个基因表达下调。

项目成果

Accuracy of an array comparative genomic hybridization (CGH) technique in detecting DNA copy number aberrtions.

阵列比较基因组杂交 (CGH) 技术检测 DNA 拷贝数畸变的准确性。

• 发表时间: 2004
• 期刊: Cancer Genetics and Cytogenetics 150
• 作者: Seiji Yano;Hideyasu Matsuyama;Kenji Matsuda;Hiroaki Matsumoto;Satoru Yoshihiro;Katsusuke Naito
• 通讯作者: Katsusuke Naito

内藤克輔: "前立腺疾患の臨床-良性疾患から前立腺癌まで-前立腺癌 治療 化学療法"日本臨床. 60. 205-210 (2002)

Katsusuke Naito：“前列腺疾病的临床检查 - 从良性疾病到前列腺癌 - 前列腺癌治疗化疗”日本临床。 60. 205-210 (2002)

DNA sequence copy number aberrations in prostate cancers : a comparison of comparative genomic hybridization data between Japan and European countries

前列腺癌中的DNA序列拷贝数畸变：日本和欧洲国家之间比较基因组杂交数据的比较

• 发表时间: 2004
• 期刊: Cancer Genetics and Cytogenetics 152
• 作者: Matsuda K.;et al.
• 通讯作者: et al.

Accuracy of an array comparative genomic hybridization (CGH) technique in detecting DNA copy number aberrations: comparison with conventional CGH and loss of heterozygosity analysis in prostate cancer

• DOI: 10.1016/j.cancergencyto.2003.09.004
• 发表时间: 2004-04-15
• 期刊: CANCER GENETICS AND CYTOGENETICS
• 作者: Yano, S;Matsuyama, H;Naito, K
• 通讯作者: Naito, K

Matsuda, K. et al.: "DNA sequence copy number aberrations in Japanese prostate cancers : a comparison of CGH data between Japan and European countries"Cancer Genet.Cytogenet.. (in press). (2004)

Matsuda, K. 等人：“日本前列腺癌中的 DNA 序列拷贝数畸变：日本和欧洲国家之间 CGH 数据的比较”Cancer Genet.Cytogenet..（出版中）。