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Mutagenicity in human bladder cancer cell line exposed to hematoporphyrin derivative photoradiation and ultraviolet Radiation

血卟啉衍生物光辐射和紫外线辐射对人膀胱癌细胞系的致突变性

基本信息

批准号：
02670701
负责人：
NAITO Katsusuke
金额：
$ 1.28万
依托单位：
Yamaguchi University School of Medicine (1991)Kanazawa University (1990)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

Cell culture studies have been performed to compare the mutagenic potential for hematoporphyrin derivative photoradiation and UV radiation. The mutation frequency in human bladder cancer cells (KK-47) at the hypoxanthine-guanine phosphoribosyltransferase locus was measured using resistance to 6-thioguanine. Treatment with UVB (wavelength ; 280-320 nm) was effective in producing mutants resistant to 6-thioguanine, but treatment with neither UVA (wavelength ; 300-430 nm) nor hematoporphirin derivative photoradiation (at comparable toxicity levels) using argon-dye laser (wavelength ; 630 nm) did not induce any mutagenic activity above background levels. Additional studies regarding hernatoporphyrin derivative UV radiation are needed. And in vivo carcinogenic studies will have to be performed to determine the long-term effects of hematoporphyrin derivative photoradiaton.

已进行细胞培养研究以比较血卟啉衍生物光辐射和紫外线辐射的致突变潜力。使用对6-硫代鸟嘌呤的抗性测量人膀胱癌细胞（KK-47）中次黄嘌呤-鸟嘌呤磷酸核糖基转移酶基因座处的突变频率。用UVB（波长：280-320 nm）处理可有效产生对6-硫鸟嘌呤具有抗性的突变体，但用UVA（波长：300-430 nm）或使用氩染料激光（波长：630 nm）的血卟啉衍生物光辐射（毒性水平相当）处理均未诱导任何高于背景水平的诱变活性。需要对hernatoporphyrin衍生物紫外线辐射进行进一步研究。血卟啉衍生物光辐射的长期效应有待于进一步的体内致癌研究。