Mutagenicity in human bladder cancer cell line exposed to hematoporphyrin derivative photoradiation and ultraviolet Radiation

细胞培养研究比较了血卟啉衍生物光辐射和紫外线辐射的致突变潜力。利用对6-硫鸟嘌呤的抗性测定了人膀胱癌细胞KK-47次黄嘌呤-鸟嘌呤磷酸核糖基转移酶位点的突变频率。UVB（波长280-320 nm）处理可有效产生对6-硫鸟嘌呤具有抗性的突变体，但UVA（波长300-430 nm）和使用氩染料激光（波长630 nm）的血卟啉衍生物光辐射（毒性水平相当）处理均未诱导任何高于背景水平的致突变活性。需要对卟啉衍生物的紫外线辐射进行进一步的研究。为了确定血卟啉衍生物光照射的长期影响，必须进行体内致癌研究。

Cell culture studies have been performed to compare the mutagenic potential for hematoporphyrin derivative photoradiation and UV radiation. The mutation frequency in human bladder cancer cells (KK-47) at the hypoxanthine-guanine phosphoribosyltransferase locus was measured using resistance to 6-thioguanine. Treatment with UVB (wavelength ; 280-320 nm) was effective in producing mutants resistant to 6-thioguanine, but treatment with neither UVA (wavelength ; 300-430 nm) nor hematoporphirin derivative photoradiation (at comparable toxicity levels) using argon-dye laser (wavelength ; 630 nm) did not induce any mutagenic activity above background levels. Additional studies regarding hernatoporphyrin derivative UV radiation are needed. And in vivo carcinogenic studies will have to be performed to determine the long-term effects of hematoporphyrin derivative photoradiaton.