Mutagenicity in human bladder cancer cell line exposed to hematoporphyrin derivative photoradiation and ultraviolet Radiation

已经进行了细胞培养研究，以比较血卟啉衍生物光辐射和紫外线辐射的诱变潜力。用6-硫代鸟嘌呤耐药法检测人膀胱癌细胞(KK-47)次黄嘌呤-鸟嘌呤磷酸核糖转移酶基因突变频率。UVB(波长：280-320 nm)处理对6-硫鸟嘌呤产生抗性突变是有效的，但用UVA(波长：300-430 nm)或用Ar-染料激光(波长：630 nm)进行的UVA(波长：630 nm)光辐射都不能诱导任何高于本底水平的诱变活性。此外，还需要对氢化卟啉衍生物的紫外线辐射进行研究。还必须进行体内致癌研究，以确定血卟啉衍生物光疗的长期效果。

Cell culture studies have been performed to compare the mutagenic potential for hematoporphyrin derivative photoradiation and UV radiation. The mutation frequency in human bladder cancer cells (KK-47) at the hypoxanthine-guanine phosphoribosyltransferase locus was measured using resistance to 6-thioguanine. Treatment with UVB (wavelength ; 280-320 nm) was effective in producing mutants resistant to 6-thioguanine, but treatment with neither UVA (wavelength ; 300-430 nm) nor hematoporphirin derivative photoradiation (at comparable toxicity levels) using argon-dye laser (wavelength ; 630 nm) did not induce any mutagenic activity above background levels. Additional studies regarding hernatoporphyrin derivative UV radiation are needed. And in vivo carcinogenic studies will have to be performed to determine the long-term effects of hematoporphyrin derivative photoradiaton.