Analysis of the interaction between fetal cells and maternal cells in placenta formation using green-mouse embryos

为了确定胎儿细胞和母体细胞在胎盘形成中的关系，我们启动了这个项目。幸运的是，这个机构的一个实验室好心地为我们准备了绿色老鼠。我们尝试建立了3D文化系统。我们试图为这个目的寻找最佳条件，但到目前为止还没有成功。和他们一起，我们表演了很多相关的作品。在卵的发育过程中，Rho的抑制剂Y27632在空洞形成前诱导发育停滞，这是可逆的。我们确定Na+/K+交换器在空洞形成中起着关键作用。我们还阐明了胎盘细胞系在低氧条件下通过HIF-1α与GLUT1启动子的一个共同的HRE位点相互作用而上调GLUT1和VEGF，以及低氧诱导的RhoA上调可能在调节滋养层细胞HIF-1α的表达中起重要作用。我们还阐明了通过抑制Rho系统来阻止入侵和渗透。阻断Rho系统的阿伦磷酸钠抑制了LPA诱导的卵巢癌转移过程中的黏附和侵袭。如上所述，这个项目在开始时也有同样的困难，但最终发表了许多与该项目相关的工作。在今后对胎盘形成的研究中，应使用来自绿色小鼠的胎盘组织。

To determine the relationship between fetal cells and maternal cells in placenta formation, we started this project. Fortunately a laboratory of this institution kindly prepared green mouse for us. We tried to set up 3D culture system. We tried to search optimal conditions for that purpose, which is no successful up to now. Along with them, we performed a lot of related works. During the development of eggs, Y27632, an inhibitor of Rho induced the arrest of development before cavity formation, which was reversible. We determined that Na+/K+exchanger had an critical role in cavity formation. We also clarified that GLUT1 and VEGF were up-regulated under low oxygen conditions in the placental cell lines through HIF-1alpha interaction with a consensus HRE site of the GLUT1 promoter and that up-regulation of RhoA induced by low-oxygen conditions may play an important role in regulation of HIF-1alpha expression in trophoblast cells. We also clarified that invasion and infiltration were blocked by inhibition of Rho system. Alendronate, which blocks Rho system, inhibited adhesion and infiltration in the dissemination morel of ovarian cancer induced by LPA.As mentioned above, this project has same difficulties in the beginning, however a lot of works were finally published in relation to this project. In the future study on placenta formation, placental tissue from the green mouse should be used.