Analysis of the interaction between fetal cells and maternal cells in placenta formation using green-mouse embryos

利用绿鼠胚胎分析胎盘形成过程中胎儿细胞和母体细胞之间的相互作用

• 批准号: 14370532
• 负责人: TASAKA Keiichi
• 金额: $ 7.94万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370532/
• 关键词: blastcyst; chirion; low-oxygen condition; hypoxia-inducible factor; ovarian cancer; dissemination; Rho; hypoxia-inducible factor 1α; ES細胞; 卵; グリーンマウス; マトリゲル

To determine the relationship between fetal cells and maternal cells in placenta formation, we started this project. Fortunately a laboratory of this institution kindly prepared green mouse for us. We tried to set up 3D culture system. We tried to search optimal conditions for that purpose, which is no successful up to now. Along with them, we performed a lot of related works. During the development of eggs, Y27632, an inhibitor of Rho induced the arrest of development before cavity formation, which was reversible. We determined that Na+/K+exchanger had an critical role in cavity formation. We also clarified that GLUT1 and VEGF were up-regulated under low oxygen conditions in the placental cell lines through HIF-1alpha interaction with a consensus HRE site of the GLUT1 promoter and that up-regulation of RhoA induced by low-oxygen conditions may play an important role in regulation of HIF-1alpha expression in trophoblast cells. We also clarified that invasion and infiltration were blocked by inhibition of Rho system. Alendronate, which blocks Rho system, inhibited adhesion and infiltration in the dissemination morel of ovarian cancer induced by LPA.As mentioned above, this project has same difficulties in the beginning, however a lot of works were finally published in relation to this project. In the future study on placenta formation, placental tissue from the green mouse should be used.

为了确定胎盘形成中胎儿细胞和母体细胞之间的关系，我们开始了这个项目。幸运的是，该机构的一个实验室好心地为我们准备了绿色小鼠。我们尝试建立3D文化体系。我们试图为此目的寻找最佳条件，但迄今为止还没有成功。沿着他们，我们进行了很多相关的工作。在卵的发育过程中，Rho的抑制剂Y27632诱导卵的发育在腔形成之前停止，这是可逆的。我们确定Na+/K+交换器在空腔形成中具有关键作用。我们还阐明了低氧条件下胎盘细胞系中GLUT 1和VEGF通过HIF-1 α与GLUT 1启动子的共有HRE位点的相互作用而上调，并且低氧条件诱导的RhoA上调可能在滋养层细胞中HIF-1 α表达的调节中起重要作用。我们还阐明了Rho系统的抑制可阻断入侵和渗透。阿仑膦酸钠能阻断Rho系统，抑制LPA诱导的卵巢癌播散性羊肚菌的粘附和浸润。如上所述，这个项目在开始时也有同样的困难，但是最终发表了很多与这个项目相关的工作。在未来的胎盘形成研究中，应使用绿色小鼠的胎盘组织。

项目成果

Ohmichi, M., et al.: "Rapid changes of flow-mediated dilatation after surgical menopause"Maturitas. 44. 125-131 (2003)

Ohmichi, M. 等人：“手术绝经后血流介导的扩张的快速变化”Maturitas。

Geranylgeranylacetone inhibits lysphosphatidic acid-induced invasion of human ovarian cancer cells in vitro

香叶基香叶基丙酮抑制溶血磷脂酸诱导的体外人卵巢癌细胞的侵袭

• 发表时间: 2005
• 期刊: Cancer 103
• 作者: K.Hashimoto.;et al.
• 通讯作者: et al.

Rho-kinase is involved in mouse blastocyst cavity formation

Rho-激酶参与小鼠囊胚腔的形成

• 发表时间: 2004
• 期刊: Biochem Biophys Res Commun 319
• 作者: R.Kawagishi;et al.
• 通讯作者: et al.

Alendronate inhibits intraperitoneal dissemination in in vivo ovarian cancer model.

• DOI: 10.1158/0008-5472.540.65.2
• 发表时间: 2005-01
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: Kae Hashimoto;Ken‐ichirou Morishige;Kenjiro Sawada;M. Tahara;Rikako Kawagishi;Y. Ikebuchi;M. Sakata;K. Tasaka;Y. Murata

Epidermal growth factor enhances invasive activity of BeVo choriocarcinoma cells by inducing α2 integrin expression

表皮生长因子通过诱导α2整合素表达增强BeVo绒毛膜癌细胞的侵袭活性

• 发表时间: 2003
• 期刊: Endocrine J 50
• 作者: Nakatsuji;Y.;et al.
• 通讯作者: et al.