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Determination of the target cells of activins and the mechanisms of FSH secretion stimulated by activins

激活素靶细胞的确定及激活素刺激FSH分泌的机制

基本信息

批准号：
06671651
负责人：
TASAKA Keiichi
金额：
$ 1.15万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671651/
关键词：
activin GH PRL LH FSH GnRH TRH CRH GHRH 下垂体細胞内カルシウムイオン

项目摘要

The purpose of the study is to clarify the mechanisms of the action of activin in the pituitary gland. Activin stimulates FSH secretion without eny effect on LH secretion. But the mechaanisms of the action how activin stimulates FSH secretion specifically is unknown. This sutdy was performed to clarify the mechanisms of the complicated effect of activin using FACS and fluorescence microscope system. The results of the study may have a important roles in reproductive medicine.The target cells of the activin A,AB,B were characterized by their responses in [Ca^<2+>]_i to each Hyopothalamic releasing hormone. 50-70% of the target cells of them were proved to be CHRH,and 40-7-%, TRH-respondirig cells. The results using purified cells by cell sorter and laser treatment were similar. Moreover some of tie activin-responding cells have response to both GHRH and TRH.Then we tired to determine the relationship between PRL secreting cells, TRH-responding cells, and gonadotrophs. The results of dot bloting assey revealed the populations of the LH and FSH secreting cells in the PRL secreting cells were 6.23% and 5.91% respectively, while the populations of the PRL secreting cells in the LH and FSH secreting cell were 18.4% and 15.5% respectively. GnRH as well as TRH increased [Ca^<2+1>]_i in the PRL secreting cells as determined by RHPA.These data suggests theat the target cells which transform itself to FSH-secretinng cells stimulated by the activin are in a subpopulation of GH or PRL-secreting cells. The process of the transformation of these cells was not clarified becaouse of the small population of these cells.

该研究的目的是阐明激活素在垂体中的作用机制。激活素刺激 FSH 分泌，但对 LH 分泌没有任何影响。但激活素具体刺激FSH分泌的作用机制尚不清楚。本研究旨在利用 FACS 和荧光显微镜系统阐明激活素复杂作用的机制。该研究结果可能在生殖医学中具有重要作用。激活素A、AB、B的靶细胞通过其对每种下丘脑释放激素的[Ca^<2+>]_i反应来表征。其中50-70%的靶细胞被证明是CHRH，40-7%是TRH反应细胞。通过细胞分选仪和激光处理纯化的细胞的结果相似。此外，一些激活素反应细胞对GHRH和TRH都有反应。然后我们疲于确定PRL分泌细胞、TRH反应细胞和促性腺激素之间的关系。斑点杂交结果显示，PRL分泌细胞中LH和FSH分泌细胞的数量分别为6.23%和5.91%，而LH和FSH分泌细胞中PRL分泌细胞的数量分别为18.4%和15.5%。通过RHPA测定，GnRH和TRH增加PRL分泌细胞中的[Ca^2+1]_i。这些数据表明，在激活素刺激下自身转化为FSH分泌细胞的靶细胞处于GH或PRL分泌细胞的亚群中。由于这些细胞的数量很少，因此这些细胞的转化过程尚不清楚。