Preparation of Molecularly Imprinted Polymers for Bio-macromolecules and Their Applications

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The aim of this study is to develop the novel preparation method of uniformly sized, molecularly imprinted polymers(MIPs) and to apply the method for the preparation of MIPs for a biomacromolecule such as a protein or nucleic acid. We tried to develop a novel preparation method using a glass or silica membrane with a relatively uniform, pore. First, a Shirasu porous glass(SPG) or porous silica membrane was reacted with octadecyltrimethylchlorosilane. Then a mixture of glyceroldimethacrylate, acrylamide, methanol and water was emulsified through hydrophobilized SPG or silica membranes in the presence of oxytocin as a template molecule. The emulsion of monomers obtained was then thermally polymerized. The obtained MIPs prepared using porous silica membranes gave more uniformly sized particles than those using SPG membranes. With regard to molecular recognition properties, the MIPs prepared by both methods gave molecular recognition for oxytocin, compared with non-imprinted polymers. Further studies are required to apply the proposed method for the preparation of MIPs for a biomacromolecule such as a protein or nucleic acid.