Preparation of Molecularly Imprinted Polymers for Bio-macromolecules and Their Applications

生物大分子分子印迹聚合物的制备及其应用

• 批准号: 14370734
• 负责人: HAGINAKA Jun
• 金额: $ 9.09万
• 依托单位: MUKOGAWA WOMEN'S UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370734/
• 关键词: molecularly imprinted polymer; biomacromolecule; reversed micelle technique

The aim of this study is to develop the novel preparation method of uniformly sized, molecularly imprinted polymers(MIPs) and to apply the method for the preparation of MIPs for a biomacromolecule such as a protein or nucleic acid. We tried to develop a novel preparation method using a glass or silica membrane with a relatively uniform, pore. First, a Shirasu porous glass(SPG) or porous silica membrane was reacted with octadecyltrimethylchlorosilane. Then a mixture of glyceroldimethacrylate, acrylamide, methanol and water was emulsified through hydrophobilized SPG or silica membranes in the presence of oxytocin as a template molecule. The emulsion of monomers obtained was then thermally polymerized. The obtained MIPs prepared using porous silica membranes gave more uniformly sized particles than those using SPG membranes. With regard to molecular recognition properties, the MIPs prepared by both methods gave molecular recognition for oxytocin, compared with non-imprinted polymers. Further studies are required to apply the proposed method for the preparation of MIPs for a biomacromolecule such as a protein or nucleic acid.

这项研究的目的是开发新型的制备方法，即均匀尺寸的分子印刷聚合物（MIP），并应​​用该方法以制备MIPS用于生物乳清分子（例如蛋白质或核酸）。我们试图使用具有相对均匀的孔的玻璃或二氧化硅膜开发一种新型的制备方法。首先，将Shirasu多孔玻璃（SPG）或多孔二氧化硅膜与八甲基三甲基氯硅烷反应。然后在催产素作为模板分子的情况下，通过疏水的SPG或二氧化硅膜将甘油甲基丙烯酸酯，丙烯酰胺，甲醇和水的混合物通过疏水的SPG或二氧化硅进行了乳化。然后将获得的单体乳液热聚合。使用多孔二氧化硅膜制备的MIP比使用SPG膜的颗粒更均匀。关于分子识别特性，与非刻印聚合物相比，通过两种方法制备的MIP都给出了催产素的分子识别。需要进一步的研究以将提出的方法应用于生物分子（例如蛋白质或核酸）的MIP制备。

项目成果

Qiang Fu: "Uniformly sized molecularly imprinted polymer for (S)-nilvadipine. Comparison of chiral recognition ability with HPLC chiral stationary phases based on a protein"Anal.Chem.. 75. 191-198 (2003)

付强：“(S)-尼伐地平的均匀尺寸分子印迹聚合物。与基于蛋白质的 HPLC 手性固定相的手性识别能力比较”Anal.Chem. 75. 191-198 (2003)

Ken Hosoya, Kimihiro Yoshizako, Takuya Kubo, Tohru Ikegami, Nobuo Tanaka, Jun Haginaka: "Selective surface modification techniqu for improvement of chromatgraphic separation selectivity for sugar derivatives"Anal.Sci.. 18. 55-58 (2002)

Ken Hosoya、Kimihiro Yoshizako、Takuy​​a Kubo、Tohru Ikegami、Nobuo Tanaka、Jun Haginaka：“用于提高糖衍生物色谱分离选择性的选择性表面改性技术”Anal.Sci.. 18. 55-58 (2002)

Haruyo Sanbe, Jun Haginaka: "Restricted access media-molecularly imprinted polymer for propranolol and its application to direct injection analysis of b-blockers in biological fluids"Analyst. 128. 593-597 (2003)

Haruyo Sanbe、Jun Haginaka：“普萘洛尔的限制进入介质分子印迹聚合物及其在生物体液中 b 阻滞剂直接注射分析中的应用”分析师。

Yoshiyuki Watabe: "Improved detectablity with a polymer-based trapping device in rapid HPLC analysis for ultra-low levels of bisphenol A (BPA) in eviromental samples"Anal.Sci.. 20. 133-137 (2004)

Yoshiyuki Watabe：“在快速 HPLC 分析环境样品中超低水平的双酚 A (BPA) 时，使用基于聚合物的捕获装置提高了可检测性”Anal.Sci. 20. 133-137 (2004)

Haruyo Sanbe: "Restricted assess media-molecularly imprinted polymer for propranolol and its application to direct injection analysis of β-blockers in biological fluids"Analyst. 128. 593-597 (2003)

Haruyo Sanbe：“普萘洛尔介质分子印迹聚合物的限制性评估及其在生物体液中 β 受体阻滞剂直接注射分析中的应用”分析师 128. 593-597 (2003)