Relationship between ultrastructural profiles of membrane systems involved in excitation-contraction coupling and signal transduction in skeletal muscle cells following exercise training

运动训练后参与骨骼肌细胞兴奋-收缩耦合和信号转导的膜系统超微结构之间的关系

基本信息

项目摘要

The t-tubules and SR are structurally and functionally joined at Ca^<2+> release units(CRUs), known as triads. We have studied the effects of short term denervation followed by reinnervation on the ultrastructural architectures in CRUs of fast-twitch(FT) and slow-twitch(ST) muscle fibers. Ischiadic nerve freezing resulted in total lack of neuromuscular transmission for 3 days followed by a slow recovery, but no decline in twitch force elicited by direct stimulation. The latter measurements indicate no significant atrophy within this time frame. The membrane systems of skeletal muscle fibres were visualized using a high voltage electron microscope. [^3H]nitrendipine binding was used to detect levels of dihydropyridine receptor(DHPR) expression. The Ca^<2+> pumping free sarcoplasmic reticulum domains were not affected by the denervation, but the Ca^<2+> release domains were dramatically increased, particularly in the FT fibres. The increase is evidenced by a doubling up of the areas of contacts between SR and t-tubules, so that in place of the normal triadic arrangement, pentadic and heptadic junctions, formed by multiple interacting layers of ST and t-tubules are seen. Frequency of pentads and heptads increases and declines in parallel to the denervation and reinnervation but with a delay. Immunofluorecence and electron microscopy observations show presence of DHPR and ryanodine receptor clusters at pentads and heptads junctions. A significant positive correlation between the level of [^3H]nitrendipine binding component and the frequency pentads and heptads was observed in both the FT and ST fibres indicating that overexpression of DHPRs accompanies the build up extra junctional contacts. It is possible that the multiple sites of pentad and heptad formation appear in response to muscle disuse to compensate for the normal CRUs, thus acting to increase the released Ca^<2+> for muscular contraction from the SR.
t-小管和SR在结构上和功能上通过Ca^2+释放单位(CRU)连接,称为三联体。我们研究了短期去神经支配后神经再支配对快收缩(FT)和慢收缩(ST)肌纤维CRU超微结构的影响。缺血性神经冷冻导致神经肌肉传递完全缺乏3天,随后恢复缓慢,但没有下降,由直接刺激引起的抽搐力。后者的测量表明在该时间范围内没有显著的萎缩。用高压电子显微镜观察骨骼肌纤维的膜系统。[^3H]尼群地平结合用于检测二氢吡啶受体(DHPR)表达水平。去神经支配不影响肌浆网游离Ca^2+泵送区,但Ca^2+释放区显著增加,尤其是在FT纤维中。增加是由SR和t-小管之间的接触面积的加倍证明,因此,在正常的三重排列,五分之一和七分之一的路口,形成的ST和t-小管的多个相互作用的层被视为。五和弦和七和弦的频率增加和减少与去神经和再神经支配平行,但有延迟。免疫荧光和电子显微镜观察显示存在的DHPR和兰尼碱受体簇在pentads和heptads交界处。在FT和ST纤维中观察到[^3H]尼群地平结合组分的水平与频率五联体和七联体之间存在显著的正相关性,表明DHPR的过度表达伴随着额外连接接触的建立。五肽和七肽形成的多个位点可能是对肌肉废用的反应,以补偿正常的CRU,从而增加SR肌肉收缩所释放的Ca^2+。

项目成果

期刊论文数量(44)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Differences in electromyographic behavior of the synergistic muscles during prolonged isometric contractions at different loads(in Japanese).
不同负荷下长时间等长收缩时协同肌肌电图行为的差异(日语)。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Tamaki;H.;Kitada;K.;Kirimoto;H.;Wagatsuma;A.;Ogita;F.;Takekura;H.;Shibayama;H.
  • 通讯作者:
    H.
Alteration of trabecular bone architecture in the proximal tibia and muscle atrophy after sciatic denervation in rats(in Japanese).
大鼠坐骨神经去神经术后近端胫骨骨小梁结构的改变和肌肉萎缩(日语)。
Nishizawa, T., Tamaki, H., Kasuga, N.Takekura, H.: "Degeneration and regeneration of neuromuscular junction achitectures in rat skeletal muscle fibres damaged by bupivacaine hydrochloride."J.Muscle Res.Cell Motility. 24. 527-537 (2003)
Nishizawa, T.、Tamaki, H.、Kasuga, N.Takekura, H.:“盐酸布比卡因损伤的大鼠骨骼肌纤维中神经肌肉接头结构的变性和再生。”J.Muscle Res.Cell Motility。
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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Changes in localization and orientation of triads following selective elimination of cytoskeletal protein in rat skinned muscle fiber.
选择性消除大鼠皮肌纤维中的细胞骨架蛋白后,三联体的定位和方向发生变化。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    McGrath;K.;Nishizawa;T.;Yamashita;S.;Tamaki;H.;Saito;K.;Takekura;H.
  • 通讯作者:
    H.
ラット腓腹筋外側部表層の筋線維形態に及ぼす自発走トレーニングの影響
自主跑训练对大鼠腓肠肌外侧面肌纤维形态的影响
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TAKEKURA Hiroaki其他文献

TAKEKURA Hiroaki的其他文献

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{{ truncateString('TAKEKURA Hiroaki', 18)}}的其他基金

Bioimaging profiles in molecular and cellular adaptations of calcium release units (CRUs) in exercise trained rat skeletal muscle cells
运动训练的大鼠骨骼肌细胞中钙释放单位(CRU)的分子和细胞适应的生物成像特征
  • 批准号:
    17300210
  • 财政年份:
    2005
  • 资助金额:
    $ 9.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Morphological and ultrastructural changes of the membrane systems involved in excitation-contraction coupling in skeletal muscle during contraction
收缩过程中参与骨骼肌兴奋-收缩耦合的膜系统的形态和超微结构变化
  • 批准号:
    11558003
  • 财政年份:
    1999
  • 资助金额:
    $ 9.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Effects of exercise training on the ultrastructural alterations of membrane systems involved in excitation-contraction coupling during contraction in skeletal muscle.
运动训练对骨骼肌收缩过程中参与兴奋-收缩耦合的膜系统超微结构改变的影响。
  • 批准号:
    10480009
  • 财政年份:
    1998
  • 资助金额:
    $ 9.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Relationships between excitation-contraction coupling deficiency and ultrastructural features of internal membrane systems in skeletal muscle fibers with aging
衰老过程中兴奋-收缩耦合缺陷与骨骼肌纤维内膜系统超微结构特征的关系
  • 批准号:
    08680130
  • 财政年份:
    1996
  • 资助金额:
    $ 9.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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仿生膜系统中可电离药物及其与树枝状聚合物的缀合物的机理分析和反应控制
  • 批准号:
    19K05541
  • 财政年份:
    2019
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    18H03536
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    2018
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    Grant-in-Aid for Challenging Research (Exploratory)
Pressure Modeling of a Permylene Membrane System
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  • 批准号:
    510924-2017
  • 财政年份:
    2017
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Role of endoplasmic reticulum-plasma membrane system in regulation of Oryza sativa endosperm development
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Black Lipid Membrane system to study biophysics of ion transport across lipid membranes
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Analysis of the regulation and cycle of atypical G proteins in intracellular-membrane system
细胞内膜系统非典型G蛋白的调控和循环分析
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Pressure Modeling of a Permylene Membrane System
二甲苯膜系统的压力建模
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