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Research on the measure for defense of cyclosporiasis in water works

水厂环孢子虫病防治措施研究

基本信息

批准号：
14380278
负责人：
ISHIBASHI Yeshinobu
金额：
$ 9.54万
依托单位：
Tohoku Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

A number of cyclosporiasis is seen on Southeast Asia or South America. The possibility of so-called afferent infection disease which is carried into our country from developing countries cannot deny, either. In this research, Cyclospora (Cyclospora cayetanensis) was examined under comparison with Cryptosporidium and Giardia. Cyclospora is a chlorine tolerance protozoan and is anxious for mass outbreak.Examination mainly consists of four items.Since Cyclospora was not detected in the aquatic environment, risk was evaluated for Giardia. The evaluation pointed out that inhabitants and medical doctors are interested in protozoan diseases.Ultrasonic wave, ultraviolet disinfection, and chlorination were compared about the disinfection effect. Recently, inactivation of Cryptosporidium by ultraviolet disinfection attracts attention. The validity of ultraviolet disinfection was shown in the experiments and disinfection effect was also accepted with ultrasonic disinfection. In WHO, 10 mJ/cm^2 had determined as a measure against Cryptosporidium. Cyclospora would be also enough for inactivation with this value.The test methods of Gryptosporidium are detected existence of oocysts, and they cannot evaluate existence of infection.Moreover, infection examination with mouse and cell culture is also difficult for the check of the infection of each oocyst. As a part of the evaluation of infection, heat-treated t2ryptosporidium was evaluated combining a cultivation cell method and the PCR method. Consequently, knowledge that infection could not see with heat-treated at 50 degrees and for 90 seconds or more was acquired. This was a big result.On the other hand, Cyclospora was propagated using cell culture. This was a big result.

许多环孢子虫病见于东南亚或南美洲。从发展中国家传入我国的所谓传入性传染病的可能性也是不能否认的。本研究对环孢霉与隐孢子虫和贾第鞭毛虫进行了比较研究。环孢子虫是一种耐氯原生动物，极易发生大规模暴发。检查主要包括四个项目。由于在水环境中未检测到环孢子虫，因此对贾第鞭毛虫进行了风险评估。评估指出，居民和医生对原虫疾病很感兴趣，对超声波、紫外线消毒和氯化消毒的消毒效果进行了比较。近年来，利用紫外线消毒技术灭活隐孢子虫引起了人们的关注。实验证明了紫外线消毒的有效性，也接受了超声波消毒的效果。在世界卫生组织中，10MJ/cm^2被确定为对抗隐孢子虫的措施。环孢子虫的检测方法是检测卵囊的存在，不能评估感染的存在，而且用小鼠感染检查和细胞培养也很难检查每个卵囊的感染情况。作为感染评估的一部分，结合培养细胞法和聚合酶链式反应方法对加热处理的t2隐孢子虫进行了评估。因此，获得了在50度和90秒或更长时间的热处理下感染不能看到的知识。这是一个很大的结果。另一方面，环孢子菌是通过细胞培养繁殖的。这是一个巨大的结果。