Control of Criptosporidium parvum infection in calves by recombinant herpesvirus vector vaccine

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In host-parasite interaction, the surface proteins of both host and parasite cells play an important role and the surface antigens of the parasites are therefore logical targets for subunit vaccine development. Several surface antigens, such as P15 and P23 of Cryptosporidium parvum (C.parvum) sporozoites or merozoites have been reported previously. Since the monoclonal antibodies against these antigens can neutralize the C.parvum sporozoites, the antigens were proposed as candidates for development of recombinant vaccine to control the cryptosporidiosis. In the present study, we constructed recombinant vaccinia virus or herpesvirus wich expresses the P15 or P23 of C.parvum and demonstrated that the recombinant P15 and P23 are similar to the native P15 and P23 both in structure and antigenicity. Mice or rabbits inoculated with the recombinant viruses produced high titers of antibodies against C.parvum sporozoites. Our next step will be immunization trails with cows to check the potency of the hyperimmune colostrum to confer effective passive immunity of humans and animals against cryptosporidiosis.