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Research on the Palpation of the Cell at Femto Newton Scale by Haptic Force Feedback Device

触觉力反馈装置飞牛顿尺度细胞触诊研究

基本信息

批准号：
14380403
负责人：
MINATO Kotaro
金额：
$ 7.81万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380403/
关键词：
Optical tweezers Palpation of the cell Haptic device Force measurement Nano technology ハプテックデバイス

项目摘要

We collected the basic data of the palpation of the cell and the analyzed the mechanism of its opposing force. Aiming at the measurement of the cell distortion, we did the experiment to attach particle to a cell and acquired a three-dimensional image.1)The collection of the basic data of the palpation of the cell : We did the experiment on a various cell. The cells used for the experiment were the HUVEC (the human vascular endothelial cell), Balb (the mouse kidney cell), and Hella cell. We attached the particle to these cells using the optical tweezers, and we impressed power and we tried to calculate the elasticity of the cell. Since the small structure of the cell scatters a laser beam, we have not achieved the measurement yet. To solve the problem, we investigated a new method to measure the center position of particle from the image on the CCD camera and we developed a system.2)The mechanism of the opposing force : It is thought that the opposing force with the cell membrane, the chromosome and the deoxyribonucleic acid are caused by unknown structure of the cell. We investigated the technique to estimate the structure inside the sample with the measurement.3)The measurement of the distortion of the cell in 3-D : In the previous research, it is shown that there was small change of the internal structure during palpation of a cell. We developed a system that enables to measure the distortion of the backbone structure within the cell and its membrane during power impressing in the three dimensions. We acquired a three-dimensional image using a confocal microscope unit attached to the system. Using this system, we can reconstruct a tomogram by scanning a field lens to the axis. As for impressing power, we adopted optical tweezers and the glass microneedle. We confirmed that the elastic deformation of the cell was 3 μm when impressing 3 μN power and the cell returned to the original when removing the power.

收集了细胞触诊的基本数据，分析了细胞触诊的反力机制。针对细胞变形的测量，我们进行了将颗粒附着在细胞上并获取三维图像的实验。1）细胞触诊基本数据的收集：我们对不同的细胞进行了触诊实验。用于实验的细胞是HUVEC（人血管内皮细胞）、Balb（小鼠肾细胞）和Hella细胞。我们用光镊把粒子附着在细胞上，我们施加能量，并试图计算细胞的弹性。由于细胞的小结构散射激光束，我们还没有实现测量。为了解决这个问题，我们研究了一种新的方法来测量粒子的中心位置从图像上的CCD相机，我们开发了一个系统。2）对抗力的机制：它被认为是与细胞膜，染色体和脱氧核糖核酸的对抗力是由未知的细胞结构。3）三维细胞变形的测量：在以往的研究中，细胞的触诊过程中，细胞内部结构的变化很小。我们开发了一种系统，能够测量在三维中施加功率期间细胞及其膜内的骨架结构的变形。我们使用连接到系统的共聚焦显微镜单元获取了三维图像。使用该系统，我们可以通过扫描场透镜到轴来重建断层图像。在压印力方面，采用了光镊和玻璃微针。实验证实，施加3 μN功率时，细胞的弹性变形为3 μm，去除功率后，细胞恢复原状。