Development of hybrid matrices for bone tissue engineering scaffolds
骨组织工程支架混合基质的开发
基本信息
- 批准号:14380398
- 负责人:
- 金额:$ 9.28万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The objective in the present research is to prepare new types of biodresorbable matrices for bone regeneration.1.Anew type of ceramic-polymer biomaterial having excellent apatite-forming ability in simulated body fluid(SBF) was prepared by hot-pressing a mixture consisting of poly (-L-lactic acid)(PLA) and calcium carbonate (vaterite). The composite containing 30% vaterite showed no brittle fracture behavior and comparably high bending strength of 〜50 MPa. Apatite layer of several micrometers in thickness formed on its surface after soaking in SBF at 37℃ only for 1 day. Osteoclast culture tests showed that the composite coated with apatite has excellent bioresorbability. After incubation of human osteoblasts, mineralized bone nodule formation was seen on the surface.2.A sponge consisting of the composite skeleton, coated with bonelike hydroxycarbonate apatite(HCA) on its surface, was derived via a particle-leaching technique combined with a biomimetic processing. A compact consisting of calcium carbonate/poly(lactic acid) composite(CCPC) and sucrose formed by hot-pressing, was soaked in the simulated body fluid at 37℃. Within initial 1 h, the sucrose was completely dissolved out, resulting in the formation of large-sized pores in the compact, and subsequently, after 3 days of soaking, bonelike HCA formed on the skeleton consisting of CCPC. The formed sponge has numerous, large pores of 〜500 μm in diameter, which are connected with channels having a diameter of 〜100 μm, as well as a high porosity of 75 %.3.Hollow spheres for bone fillers incorporating cells (cell-delivery system) were prepared using CCPC. An open channel of 〜800 μm in diameter was easily formed using a chemical etching method to provide a pathway to the interior of the sphere. Cells could migrate through the open channel into the interior of the sphere.
本研究旨在制备新型生物可吸收骨再生基质材料。1.采用热压法制备了一种新型陶瓷-聚合物生物材料,该材料在模拟体液中具有良好的磷灰石形成能力。含30%球泡石的复合材料没有脆性断裂行为,弯曲强度也较高,约为50 Mpa。在37℃的体液中浸泡1d后,在其表面形成了几微米厚的磷灰石层。破骨细胞培养实验表明,磷灰石涂层复合材料具有良好的生物再吸收性能。人成骨细胞孵育后,表面可见矿化的骨结节形成。2.通过颗粒浸出技术和仿生处理相结合的方法,制备了由复合骨架组成的海绵,其表面涂覆了类骨的羟基碳酸盐磷灰石(HCA)。将碳酸钙/聚乳酸复合材料和热压蔗糖制成的复合体在37℃的模拟体液中浸泡。在最初的1h内,蔗糖被完全溶解,导致压坯中形成大尺寸的气孔,随后,在浸泡3d后,由CCPC组成的骨架上形成了骨状的HCA。形成的海绵具有大量的大孔,直径约500μm,与直径约100μm的孔道相连,孔隙率高达75%。3.采用细胞输送系统制备了可用于骨填充的中空球。使用化学蚀刻方法很容易形成直径约800μm的开放通道,以提供通向球体内部的通道。细胞可以通过开放的通道迁移到球体内部。
项目成果
期刊论文数量(116)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Preparation of Calcium Carbonate/Poly(lactic acid) Composite (CCPC) Hollow Spheres
碳酸钙/聚乳酸复合材料(CCPC)空心球的制备
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:H.Maeda;他
- 通讯作者:他
Preparation of Bioactive Polylactic Acid Composites Containing Calcium Carbonates
含碳酸钙生物活性聚乳酸复合材料的制备
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Hirotaka Maeda;他
- 通讯作者:他
H.Maeda, 他: "Preparation of Bioactive Polylactic Acid Composites Containing Calcium Carbonates"Key Engng.Mater.. 240-242. 163-166 (2003)
H.Maeda 等人:“含有碳酸钙的生物活性聚乳酸复合材料的制备”Key Enng.Mater. 240-242 (2003)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Cong Wang 他: "Preparation of Macroporous Calcium Phosphate Invert Glass-Ceramics"Proc. 3^<rd> Asian BioCeramics. 3. 355-359 (2003)
Cong Wang等人:“大孔磷酸钙反相玻璃陶瓷的制备”Proc. 3^<rd>亚洲生物陶瓷。
- DOI:
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- 影响因子:0
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HAYAKAWA Tomokatsu其他文献
HAYAKAWA Tomokatsu的其他文献
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{{ truncateString('HAYAKAWA Tomokatsu', 18)}}的其他基金
Development of transparent rare-earth magnetic oxide glasses and their optical devices
透明稀土磁性氧化物玻璃及其光学器件的研制
- 批准号:
20900120 - 财政年份:2008
- 资助金额:
$ 9.28万 - 项目类别: