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Mechanical response of chondrocyte under intermittent hydrostatic pressure loading.-Regeneration of Articular Cartilage

间歇静水压负荷下软骨细胞的机械反应-关节软骨的再生

基本信息

批准号：
15390464
负责人：
KUBO Toshikazu
金额：
$ 9.54万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Chondrocytes were isolated from the articular cartilage of rabbit joints. The cells were exposed to 0.5 Hz of intermittent hydrostatic pressure(IHP) ranging from 1-5 MPa for 1 hr. After 30 minutes of pressure loading, total RNA was extracted and analyzed by competitive RT-PCR for mRNA expression relating to cartilage metabolism. The expression of proteoglycan did not changed following the exposure to 5 MPa of IHP, but the expression of collagen type II markedly increased. The expression of collagen type I and X were not detected in all cells. These results showed that the exposure to 5 MPa of IHP could inhibit terminal differentiation of chondrocytes and enhance cartilage metabolism. Five MPa of IHP had positive effect on cartilage metabolism.Human periodontal ligament(hPDL) cells were also loaded to 0.5 Hz of IHP ranging from 1-6 MPa for 1 hr. The exposure to 6 MPa of IHP enhanced the expression of IL-6, IL-8, RANKL and OPG. IL-6 and IL-8 are inflammatory cytokines, and RANKL and OPG are cytokines relating to osteoclasts differentiation. Six MPa of hydrostatic pressure is reported as a human physiological occlusal pressure level. These results demonstrated that physiological IHP had effect on inflammatory cytokines expression and bone metabolism.These findings provide a clue to elucidation of a part of the pathogenesis of osteoarthritis.

从兔关节的关节软骨中分离出软骨细胞。将细胞暴露于 1-5 MPa 范围内的 0.5 Hz 间歇静水压 (IHP) 下 1 小时。压力加载30分钟后，提取总RNA并通过竞争性RT-PCR分析与软骨代谢相关的mRNA表达。暴露于5 MPa IHP后，蛋白多糖的表达没有变化，但II型胶原的表达显着增加。并非所有细胞中均检测到 I 型和 X 型胶原蛋白的表达。这些结果表明，暴露于5 MPa的IHP可以抑制软骨细胞的终末分化并增强软骨代谢。 5 MPa 的 IHP 对软骨代谢有积极作用。人牙周膜 (hPDL) 细胞也被加载到 1-6 MPa 范围内的 0.5 Hz IHP 中 1 小时。暴露于6 MPa IHP可增强IL-6、IL-8、RANKL和OPG的表达。 IL-6和IL-8是炎症细胞因子，RANKL和OPG是与破骨细胞分化相关的细胞因子。据报告，6 MPa 的静水压力为人体生理咬合压力水平。这些结果表明生理性IHP对炎症细胞因子的表达和骨代谢有影响。这些结果为阐明骨关节炎的部分发病机制提供了线索。