The analysis of cellular stress response towards novel conservative therapies for joint disease

关节疾病新型保守疗法的细胞应激反应分析

• 批准号: 20390404
• 负责人: KUBO Toshikazu
• 金额: $ 12.4万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390404/
• 关键词: heat shock protein 70; ストレス応答機構; 変形性関節症; 低酸素; 温熱; グルタミン; マイクロウェーブ; 関節疾患; HSP70; 低酸素誘導因子; 骨形成因子

When heat shock protein 70(HSP70), a molecular chaperone, was induced in chondrocytes as a stress response mechanism under hypoxic condition, the expression of extracellular matrix gene were increased, and the chondrocytes were protected. We developed the efficiently and safe methods that supplementation of glutamine in combination with moderate heat treatment using a microwave applicator induced sufficient increased of HSP70 for therapeutic purposes in vivo. These findings showed that controlling HSP70 expression in the cartilage using supplementation of glutamine in combination with thermotherapy might offer a novel strategy for suppressing cartilage degeneration in osteoarthritis.

低氧条件下诱导软骨细胞产生热休克蛋白70（HSP 70）作为应激反应机制，可增加细胞外基质基因的表达，从而保护软骨细胞。我们开发了一种有效和安全的方法，即补充谷氨酰胺与使用微波器的适度热处理相结合，诱导足够的HSP 70增加用于治疗目的。这些发现表明，通过补充谷氨酰胺结合热疗来控制软骨中HSP 70的表达可能为抑制骨关节炎中的软骨退变提供了一种新的策略。

项目成果

N-Acetylcysteine Prevents Nitric Oxide-Induced Chondrocyte Apoptosis and Cartilage Degeneration in an Experimental Model of Osteoarthritis

• DOI: 10.1002/jor.20976
• 发表时间: 2010-02-01
• 期刊: JOURNAL OF ORTHOPAEDIC RESEARCH
• 影响因子: 2.8
• 作者: Nakagawa, Shuji;Arai, Yuji;Kubo, Toshikazu
• 通讯作者: Kubo, Toshikazu

低酸素環境およびHIF-1αが培養軟骨におけるHSP70と軟骨細胞外基質合成に与える影響

缺氧环境和HIF-1α对培养软骨中HSP70和软骨细胞外基质合成的影响

• 发表时间: 2011
• 作者: 土田真嗣;新井祐志;寺内竜;本城邦晃;中川周士;平岡延之;井上裕章;齊藤正純;松田修;久保俊一
• 通讯作者: 久保俊一

Hyperthermia for the treatment of articular cartilage with osteoarthritis

• DOI: 10.3109/02656730903107519
• 发表时间: 2009-01
• 期刊: International Journal of Hyperthermia
• 影响因子: 3.1
• 作者: Kenji A. Takahashi;H. Tonomura;Y. Arai;R. Terauchi;K. Honjo;N. Hiraoka;T. Hojo;Taisuke Kunitomo;T. Kubo

Effects of heat stimulation via microwave appliocator on cartilage matrix gene and HSP70 expression in the rabbit knee joint

微波热刺激对兔膝关节软骨基质基因及HSP70表达的影响

• 发表时间: 2008
• 期刊: Journal of Orthopaedic Reseach 26(1)
• 作者: Hitoshi Tonomura;Toshikazu Kubo
• 通讯作者: Toshikazu Kubo

Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint

• DOI: 10.1002/jor.20421
• 发表时间: 2008-01
• 期刊: Journal of Orthopaedic Research
• 影响因子: 2.8
• 作者: H. Tonomura;Kenji A. Takahashi;O. Mazda;Y. Arai;M. Shin-Ya;A. Inoue;K. Honjo;T. Hojo;J. Imanishi;T. Kubo