The analysis of cellular stress response towards novel conservative therapies for joint disease

在低氧条件下，作为应激反应机制的分子伴侣热休克蛋白70(HSP70)在软骨细胞中被诱导后，细胞外基质基因表达增加，对软骨细胞具有保护作用。我们开发了一种安全有效的方法，即补充谷氨酰胺，联合使用微波加热，在体内诱导足够多的HSP70表达，以达到治疗目的。这些结果表明，补充谷氨酰胺结合热疗控制HSP70在软骨中的表达可能为抑制骨关节炎软骨退变提供一种新的策略。

When heat shock protein 70(HSP70), a molecular chaperone, was induced in chondrocytes as a stress response mechanism under hypoxic condition, the expression of extracellular matrix gene were increased, and the chondrocytes were protected. We developed the efficiently and safe methods that supplementation of glutamine in combination with moderate heat treatment using a microwave applicator induced sufficient increased of HSP70 for therapeutic purposes in vivo. These findings showed that controlling HSP70 expression in the cartilage using supplementation of glutamine in combination with thermotherapy might offer a novel strategy for suppressing cartilage degeneration in osteoarthritis.