Course of SARS coronavirus-2-specific antibody titers and magnitude of the memory B-cell and T-cell response

SARS 冠状病毒 2 特异性抗体滴度的变化过程以及记忆 B 细胞和 T 细胞反应的强度

• 批准号: 458313848
• 负责人: Professor Dr. Christian Jassoy
• 金额: --
• 依托单位: Institut für Virologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/458313848?language=en
• 关键词: Course; SARS; coronavirus; specific; antibody

Most or all SARS CoV-2-infected individuals develop an immune response to the virus. The goal of the study is to examine the course of the antibody titers and the magnitude of the virus-specific memory B-cell and T-cell responses in individuals with mild to moderate SARS CoV-2 infection.Fifty to sixty adult study participants with SARS CoV-2 infection will be followed up for 12 months. Four to ten weeks, 6, 9 and 12 months after infection serum samples will be obtained. In addition, at 9 and/or 12 months, heparinized blood specimens will be obtained to prepare mononuclear cells. Sera will be used to examine the antibody responses against the SARS CoV-2 nucleoprotein and the receptor binding domain (RBD) of the S glycoprotein, influenza virus nucleoprotein and tetanus toxoid as well as the neutralizing antibody response. Cells will be used to measure the magnitude of the SARS CoV-2 nucleoprotein and RBD, influenza virus nucleoprotein-, cytomegalovirus-, and tetanus toxoid-specific memory B- and T-cell response. The T-cell response against the RBD will be observed in greater detail with single overlapping peptides.The study measures the course of the SARS CoV-2-specific antibody response in the first six months and later than six months of infection and the magnitude of the virus-specific memory B- and T-cell response at 9-12 months after infection. The immune responses will be put into perspective to each other and to immune responses against influenza virus, cytomegalovirus and tetanus antigens. A special focus of the study is the association of the neutralizing antibody response with antibody, memory B- and T-cell responses against the receptor binding domain of the viral glycoprotein.

大多数或所有感染SARS CoV-2的人对病毒产生免疫反应。这项研究的目的是检测轻到中度SARS CoV-2感染患者的抗体滴度和病毒特异性记忆B细胞和T细胞反应的大小。将对50至60名感染SARS CoV-2的成人研究参与者进行为期12个月的随访。于感染后4~10周、6、9、12个月采集血清标本。此外，在9个月和/或12个月时，将获得肝素化的血液样本，以准备单个核细胞。血清将用于检测针对SARS CoV-2核蛋白和S糖蛋白、流感病毒核蛋白和破伤风类毒素的受体结合域的抗体反应以及中和抗体反应。这些细胞将被用来测量SARS CoV-2核蛋白和RBD、流感病毒核蛋白、巨细胞病毒和破伤风毒素特异性记忆B和T细胞反应的大小。针对RBD的T细胞反应将通过单一重叠多肽进行更详细的观察。这项研究测量了SARS CoV-2特异性抗体反应在感染后前六个月和之后六个月的过程，以及感染后9-12个月病毒特异性记忆B细胞和T细胞反应的幅度。免疫反应将被放在彼此之间以及针对流感病毒、巨细胞病毒和破伤风抗原的免疫反应中。这项研究的一个特别重点是中和抗体反应与针对病毒糖蛋白受体结合域的抗体、记忆B细胞和T细胞反应的关系。