Elucidation of the mammalian mitochondrial respiration mechanism at the atomic level.

牛心脏细胞色素氧化酶在氢原子水平上的x射线结构表明质子泵送途径（h途径）可接受4个质子，而时间分辨红外分析表明h途径的开/闭跃迁受O2还原位点控制。从振动光谱的角度探讨了nadh -泛醌还原酶抑制活性氧产生的机理。基本建立了FoF1ATP合酶的二维结晶条件。在两种蛋白复合物的二维结晶过程中，发现了细胞染色质c诱导CcO跨膜区构象变化。

X-ray structure of bovine heart cytochrome oxidase at the hydrogen atom level showed that the proton-pumping pathway (H-pathway) can accept 4 protons, while the time-resolved infrared analysis showed that the open/closed transition of H-pathway is controlled by the O2 reduction site. The mechanism of suppression of reactive oxygen production by NADH-ubiquinone reductase has been proposed vibrational spectroscopically. The 2D crystallization conditions for FoF1ATP synthase has been essentially established. The induction of conformationl changes in the transmembrane region of CcO by cytocrome c was discovered during the 2D crystallization of the complex of the two proteins.