Elucidation of the mammalian mitochondrial respiration mechanism at the atomic level.

在原子水平上阐明哺乳动物线粒体呼吸机制。

• 批准号: 22247012
• 负责人: YOSHIKAWA Shinya
• 金额: $ 28.29万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22247012/
• 关键词: 生体エネルギー変換; 膜タンパク質; 金属タンパク質; X線結晶構造解析; 振動分光解析

X-ray structure of bovine heart cytochrome oxidase at the hydrogen atom level showed that the proton-pumping pathway (H-pathway) can accept 4 protons, while the time-resolved infrared analysis showed that the open/closed transition of H-pathway is controlled by the O2 reduction site. The mechanism of suppression of reactive oxygen production by NADH-ubiquinone reductase has been proposed vibrational spectroscopically. The 2D crystallization conditions for FoF1ATP synthase has been essentially established. The induction of conformationl changes in the transmembrane region of CcO by cytocrome c was discovered during the 2D crystallization of the complex of the two proteins.

在氢原子水平上对牛心细胞色素氧化酶的X-射线结构进行了研究，结果表明，质子泵途径（H-途径）可以接受4个质子，而时间分辨红外光谱分析表明，H-途径的开/闭转变受O2还原位点的控制。本文从振动光谱的角度提出了NADH-泛醌还原酶抑制活性氧产生的机理。FoF 1 ATP合酶的二维结晶条件已基本建立。在两种蛋白质复合物的二维结晶过程中，发现cytocrome c诱导CcO跨膜区的构象变化。

项目成果

Reaction mechanism of cytochrome c oxidase based on the O_2 analogues-bound structures.

基于O_2类似物结合结构的细胞色素c氧化酶的反应机制。

• 发表时间: 2010
• 作者: S. Inoue;Y. Ono;T. Watanabe;S. Ito;T. Ii;S. Kamio;T. Yamada;M. Inomoto.;村本和優
• 通讯作者: 村本和優

Structural Relaxation of Cytochrome c Oxidase after CO Photolysis : Assignment of a Heme peripheral Raman Mode

CO 光解后细胞色素 c 氧化酶的结构松弛：血红素外周拉曼模式的分配

• 发表时间: 2010
• 作者: A. Kuwahata;B. Gao;T. Ito;H. Tanabe;S. Ito;M. Inomoto;Y. Ono;Izumi Ishigami
• 通讯作者: Izumi Ishigami

チトクロム酸化酵素の水素原子観測に向けて

细胞色素氧化酶的氢原子观察

• 作者: Sakajiri T;Asano K;Hirooka S;Ohnuma M;Misumi O;Yoshida M;Fujiwara T;Doi S;Kuroiwa H;Kuroiwa T;Shinya Yoshikawa;Y.Ono;吉川信也
• 通讯作者: 吉川信也

An intermediate conformational state during ligand binding to cytochrome c oxidase detected by time-resolved resonance Raman analysis of heme peripheral groups

通过血红素外周组的时间分辨共振拉曼分析检测配体与细胞色素 c 氧化酶结合期间的中间构象状态

• 发表时间: 2012
• 期刊: Chem.Lett.
• 作者: T. Ushiki;T.G Watanabe;K. Yamasaki;A. Wang;H. Ishikawa;X. Guo;H. Nakamata;H. Koguchi;M. Inomoto;Y. Ono;Izumi Ishigami
• 通讯作者: Izumi Ishigami

Proton-pumping mechanism of of cytochrome c oxidase

细胞色素c氧化酶的质子泵机制

• DOI: 10.1146/annurev-biophys-042910-155341
• 发表时间: 2011
• 期刊: Annu.Rev.Biophys.
• 作者: 原田昌朋;張継東;畠山淳;江頭恒;安部眞一;吉川信也
• 通讯作者: 吉川信也