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The atomic mechanism of the functions of protein complexes which drive mitochondrial energy transduction.

The atomic mechanism of the functions of protein complexes which drive mitochondrial energy transduction.
驱动线粒体能量转导的蛋白质复合物功能的原子机制。
批准号:
16087208
负责人:
YOSHIKAWA Shinya
金额:
$54.46万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
财政年份:
2004
资助国家:
日本
项目状态:
已结题
起止时间:
2004 至 2009

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中文摘要
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英文摘要
Complete structures of the intrinsic lipids in bovine cytochrome c oxidase were determined. His503 couples the proton-pumping process with O_2 reduction process. Resting oxidized enzyme has a peroxide bridged in the O_2 reduction site. The bound O_2^- at Fe_<a3>^<2+> is completely reduced by a non-consecutive three electron donation. The water-channel of the H-channel blocks the reverse proton leak to provide the highly effective energy coupling. Two dimensional crystals of NADH-ubiquinone reductase and F0F1ATPase were obtained. Highly sensitive infrared apparatus applicable for any protein system in aqueous solution was constructed.
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The intrinsic stability of the second intermediate following the dioxygen-bound form in the O_2 reduction by cytochrome c oxidase
细胞色素 c 氧化酶 O_2 还原中双氧结合形式的第二中间体的内在稳定性
DOI: --
发表时间: 2004
期刊: FEES Left 570
影响因子: --
作者: [Kenji Oda, et al.]
通讯作者: et al.
水溶性の複合蛋白質Pseudomonas putidaチトクロムP450camの無細胞系での合成
无细胞系统中水溶性复合蛋白恶臭假单胞菌细胞色素 P450cam 的合成
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [Y.Inoue, J.Baasansuren, M.Watanabe, H.Kamei, 金丸周司, 片山幸江]
通讯作者: 片山幸江
チトクロム酸化酵素の結晶のSoret帯吸収スペクトル測定
细胞色素氧化酶晶体的索雷带吸收光谱测量
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [阿児雄之, 関口宗克, 亀井宏行, 鷹野光行, 新田栄治, 指宿市教育委員会, 望月正雄]
通讯作者: 望月正雄
High resolution diffraction experiment of bovine cytochrome c oxidase
牛细胞色素c氧化酶的高分辨率衍射实验
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [河野俊行, ほか, Suga M]
通讯作者: Suga M
106
    Elucidation of the mammalian mitochondrial respiration mechanism at the atomic level.
    • 批准号:
      22247012
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $28.29万
    • 财政年份:
      2010
    • 负责人:
      YOSHIKAWA Shinya
    • 依托单位:
    Studies on the proton pumping in mitochondrial electrotransfer system.
    • 批准号:
      09308026
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $22.02万
    • 财政年份:
      1997
    • 负责人:
      YOSHIKAWA Shinya
    • 依托单位:
    Preparation of crystals of cytochrome c oxidase and cytochrome bcl complex
    • 批准号:
      06453220
    • 项目类别:
      Grant-in-Aid for General Scientific Research (B)
    • 资助金额:
      $4.54万
    • 财政年份:
      1994
    • 负责人:
      YOSHIKAWA Shinya
    • 依托单位:
    Folding and crystallization conditions of menbrane protein supercomplexes
    • 批准号:
      03304056
    • 项目类别:
      Grant-in-Aid for Co-operative Research (A)
    • 资助金额:
      $10.88万
    • 财政年份:
      1990
    • 负责人:
      YOSHIKAWA Shinya
    • 依托单位:
    海外基金