The atomic mechanism of the functions of protein complexes which drive mitochondrial energy transduction.
The atomic mechanism of the functions of protein complexes which drive mitochondrial energy transduction.
批准号:
16087208
负责人:
YOSHIKAWA Shinya
金额:
$54.46万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
财政年份:
2004
资助国家:
日本
项目状态:
已结题
起止时间:
2004 至 2009
中文摘要
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英文摘要
Complete structures of the intrinsic lipids in bovine cytochrome c oxidase were determined. His503 couples the proton-pumping process with O_2 reduction process. Resting oxidized enzyme has a peroxide bridged in the O_2 reduction site. The bound O_2^- at Fe_<a3>^<2+> is completely reduced by a non-consecutive three electron donation. The water-channel of the H-channel blocks the reverse proton leak to provide the highly effective energy coupling. Two dimensional crystals of NADH-ubiquinone reductase and F0F1ATPase were obtained. Highly sensitive infrared apparatus applicable for any protein system in aqueous solution was constructed.
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The intrinsic stability of the second intermediate following the dioxygen-bound form in the O_2 reduction by cytochrome c oxidase
细胞色素 c 氧化酶 O_2 还原中双氧结合形式的第二中间体的内在稳定性
DOI:
--
发表时间:
2004
期刊:
FEES Left 570
影响因子:
--
作者:
[Kenji Oda, et al.]
通讯作者:
et al.
水溶性の複合蛋白質Pseudomonas putidaチトクロムP450camの無細胞系での合成
无细胞系统中水溶性复合蛋白恶臭假单胞菌细胞色素 P450cam 的合成
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Y.Inoue, J.Baasansuren, M.Watanabe, H.Kamei, 金丸周司, 片山幸江]
通讯作者:
片山幸江
チトクロム酸化酵素の結晶のSoret帯吸収スペクトル測定
细胞色素氧化酶晶体的索雷带吸收光谱测量
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[阿児雄之, 関口宗克, 亀井宏行, 鷹野光行, 新田栄治, 指宿市教育委員会, 望月正雄]
通讯作者:
望月正雄
High resolution diffraction experiment of bovine cytochrome c oxidase
牛细胞色素c氧化酶的高分辨率衍射实验
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[河野俊行, ほか, Suga M]
通讯作者:
Suga M
IPR beamline for macromolecular assemblies at SPring-8
SPring-8 用于大分子组装的 IPR 光束线
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[亀井宏行, 阿児雄之, 古川桂, K. Tsutsui, 竹村和浩, 北尾彰朗, 西村幸夫, Hiroshi Aoyama, Toshiyuki KONO, 曽野裕夫, 樋脇 博敏, Eiki Ymashita]
通讯作者:
Eiki Ymashita
共 106 条
Elucidation of the mammalian mitochondrial respiration mechanism at the atomic level.
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批准号:22247012
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项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$28.29万
-
财政年份:2010
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负责人:YOSHIKAWA Shinya
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依托单位:
Studies on the proton pumping in mitochondrial electrotransfer system.
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批准号:09308026
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$22.02万
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财政年份:1997
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负责人:YOSHIKAWA Shinya
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依托单位:
Preparation of crystals of cytochrome c oxidase and cytochrome bcl complex
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批准号:06453220
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.54万
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财政年份:1994
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负责人:YOSHIKAWA Shinya
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依托单位:
Folding and crystallization conditions of menbrane protein supercomplexes
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批准号:03304056
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项目类别:Grant-in-Aid for Co-operative Research (A)
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资助金额:$10.88万
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财政年份:1990
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负责人:YOSHIKAWA Shinya
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依托单位:
Studies on the Reaction Mechanism of the Cytochrome oxidase based on the Crystal Structure
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批准号:02044126
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$5.44万
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财政年份:1990
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负责人:YOSHIKAWA Shinya
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依托单位:
Crystallization and X-ray crystallographic analysis of Complexes III and I in mitochondrial respiratory system
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批准号:03454565
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.39万
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财政年份:1990
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负责人:YOSHIKAWA Shinya
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依托单位:
Crysallization and x-ray crystallographic studies on bovine heart cytochrome c oxidase.
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批准号:61480479
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.65万
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财政年份:1986
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负责人:YOSHIKAWA Shinya
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依托单位:
海外基金