Comprehensive analyses of regulatory mechanisms associated with hydrolytic enzyme production and metabolic pathway involved in biomass degradation in filamentous fungi

(1)转录因子AmyR定位于细胞核，在诱导剂的存在下显着不稳定，但MalR表现出组成性核定位和稳定性，无论碳源。AmyR与伴侣Hsp 70和Hsp 90相关。(2)加入葡萄糖后，麦芽糖转运蛋白MalP靶向于液泡。HECT泛素连接酶HulA和抑制蛋白样蛋白CreD都是葡萄糖诱导的MalP内吞所必需的。此外，使用与GST融合的HulA的CreD-3FLAG和WW结构域进行的下拉分析表明，CreD可以通过WW结构域与HulA相互作用。(3)通过转录组分析对戊糖代谢相关基因进行了全面筛选，并对候选基因产物的酶学性质进行了分析，从而完整阐明了曲霉戊糖代谢途径。

(1) Transcription factor AmyR was localized to nucleus and significantly unstable in the presence of inducer, but MalR showed constitutive nuclear localization and stability irrespective of carbon sources. AmyR was associated with chaperons Hsp70 and Hsp90. (2) Maltose transporter MalP was targeted to the vacuole after addition of glucose. Both HECT ubiquitin ligase HulA and arrestin-like protein CreD are required for glucose-induced endocytosis of MalP. In addition, pull-down analysis using CreD-3FLAG and WW domains of HulA fused to GST indicated that CreD could interact with HulA via WW domains. (3) Comprehensive screening of genes involved in pentose metabolism was performed by transcriptome analysis, and enzymatic properties of candidate gene products were analyzed, which resulted in the complete elucidation of the pentose metabolic pathway in Aspergillus oryzae.