Molecular mechanisms for production of useful proteins in Aspergillus fungi based on genomic information

点击翻译按钮获取中文摘要

Based on genome database of koji-mold (Aspergillus oryzae), approximately 400 genes encoding transcription factor have been found, and a library for strains that express those factor genes at high level has been constructed. By using this library, several transcription factors involved in production of industrially important enzymes such as protease, cellulase, lipase, and so on, have been found. Comprehensive analyses of genes/proteins in signal transduction pathway have revealed the novel histidine kinase involved in anaerobic response and the first direct biochemical evidence for the existence of His-Asp phosphotransfer systems in filamentous fungi. In addition, yield of heterologous protein production has been improved by using protease-low-producer strains.