Molecular mechanisms for production of useful proteins in Aspergillus fungi based on genomic information

基于基因组信息的曲霉属真菌生产有用蛋白质的分子机制

• 批准号: 17019001
• 负责人: GOMI Katsuya
• 金额: $ 64.83万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17019001/
• 关键词: 糸状菌; コウジカビ; 転写制御因子; シグナル伝達; タンパク質分泌; マイクロアレイ; 相同組換え; 転写因子; 情報伝達; ヒスチジンキナーゼ; MAPキナーゼ; 酵素生産; 異種遺伝子発現; 小胞輸送; 有用分解酵素; 強制発現; プロテアーゼ

Based on genome database of koji-mold (Aspergillus oryzae), approximately 400 genes encoding transcription factor have been found, and a library for strains that express those factor genes at high level has been constructed. By using this library, several transcription factors involved in production of industrially important enzymes such as protease, cellulase, lipase, and so on, have been found. Comprehensive analyses of genes/proteins in signal transduction pathway have revealed the novel histidine kinase involved in anaerobic response and the first direct biochemical evidence for the existence of His-Asp phosphotransfer systems in filamentous fungi. In addition, yield of heterologous protein production has been improved by using protease-low-producer strains.

基于曲霉基因组数据库，已发现约400个编码转录因子的基因，并构建了高水平表达这些因子基因的菌株文库。利用该文库，发现了与蛋白酶、纤维素酶、脂肪酶等重要工业酶的产生有关的转录因子，并对信号转导途径中的基因/蛋白进行了综合分析，揭示了新的组氨酸激酶参与厌氧反应，为丝状真菌中His-Asp磷酸转移系统的存在提供了第一个直接的生化证据。此外，通过使用蛋白酶低生产菌株，提高了异源蛋白生产的产量。

项目成果

Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future.

• DOI: 10.1093/dnares/dsn020
• 发表时间: 2008-08
• 期刊: DNA research : an international journal for rapid publication of reports on genes and genomes
• 作者: Machida M;Yamada O;Gomi K
• 通讯作者: Gomi K

Isolation of Aspergillus oryzae mutants for heterologous protein production from a double proteinase gene disruptant

• DOI: 10.1007/s00253-008-1851-1
• 发表时间: 2009-01
• 期刊: Applied Microbiology and Biotechnology
• 影响因子: 5
• 作者: Takashi Nemoto;Taisuke Watanabe;Yutaka Mizogami;J. Maruyama;K. Kitamoto

Up-regulation of ABC transporter genes stimulated by azole drug dependent on the transcription activator AtrR in Aspergillus oryzae

米曲霉中唑类药物刺激的 ABC 转运蛋白基因上调依赖于转录激活剂 AtrR

• 发表时间: 2010
• 作者: A.Ohba;et al.
• 通讯作者: et al.

麹菌 A.oryzae の異種タンパク質生産における液胞タンパク質ソーティングレセプター遺伝子破壊の効果

米曲霉液泡蛋白分选受体基因破坏对异源蛋白产生的影响

• 发表时间: 2009
• 作者: 尹戴宇;ほか
• 通讯作者: ほか

A defect of ligD (human lig4 homolog) for nonhomologous end joining significantly improves efficiency of gene-targeting in Aspergillus oryzae

• DOI: 10.1016/j.fgb.2007.12.010
• 发表时间: 2008-06-01
• 期刊: FUNGAL GENETICS AND BIOLOGY
• 影响因子: 3
• 作者: Mizutani, Osamu;Kudo, Youhei;Gomi, Katsuya
• 通讯作者: Gomi, Katsuya