Studies on signal activating systems induced by an anorexigenicappetitekine, Nesfatin-1

Nesfatin-1剂量依赖性地促进小鼠NB41A3细胞CRE报告活性的增加，Nesfatin-1以高亲和力的方式与小鼠下丘脑和NB41A3细胞的质膜结合。使用CRE报告分析了49个gpcr，试图确定Nesfatin-1的特异性受体，但这些努力都失败了。γ激活剂曲格列酮具有显著的稳定NUCB2基因的活性，Nesfatin-1激活EGF信号通路，抑制脂肪生成。

Nesfatin-1 administration promoted a dose-dependent increase in CRE reporteractivities inmouse NB41A3cells,and Nesfatin-1 bound plasmamembranes derived frommouse hypothalami and NB41A3 cells in a high affinity manner. Using a CRE reporter assay ineachof 49 GPCRs, wetried to identify a receptorspecific to Nesfatin-1, but these efforts resulted in failure. Troglitazone, an activator for PPARγ, possessed a significantstabilizing activity of NUCB2 gene mRNA, and Nesfatin-1 activated EGF signaling tosuppress adipogenesis.