Studies on signal activating systems induced by an anorexigenicappetitekine, Nesfatin-1

Nesfatin-1可促进小鼠NB 41 A3细胞CRE的增殖，并以高亲和力结合小鼠下丘脑和NB 41 A3细胞的质膜。我们使用49个GPCR中的CRE报告基因检测，试图鉴定Nesfatin-1特异性受体，但这些努力都失败了。Troglitazone是一种PPARγ激活剂，具有显著的NUCB 2基因mRNA稳定活性，Nesfatin-1激活EGF信号抑制脂肪形成。

Nesfatin-1 administration promoted a dose-dependent increase in CRE reporteractivities inmouse NB41A3cells,and Nesfatin-1 bound plasmamembranes derived frommouse hypothalami and NB41A3 cells in a high affinity manner. Using a CRE reporter assay ineachof 49 GPCRs, wetried to identify a receptorspecific to Nesfatin-1, but these efforts resulted in failure. Troglitazone, an activator for PPARγ, possessed a significantstabilizing activity of NUCB2 gene mRNA, and Nesfatin-1 activated EGF signaling tosuppress adipogenesis.