Analysis and Clinical application of a novel satiety molecule stimulated by PPAR ganma knock out mouse

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When a feeding was restrained for amount of medicine for usage dependence by the dosage in a rat brain of this re-combination Nantes protein and administered an anti nesfatin antibody, that I am significant, food consumption increased was recognized. It was recognized that there was a lot it in hypothalamus with connection for a feeding by immunity dyeing in a rat brain by an anti nesfatin antibody. I let a rat go without food for 24 hours, and, according to the experiment that I used NUCB2 cRNA for, the NUCB2 mRNA expression decreased with room side nucleus by fast distinctly. In addition, in the dosage in a cerebral ventricle of Nesfatin-1 peptide to the Zucker rat which there was Leptin acceptor abnormality, as for the feeding restraint action, as for Nesfatin-1, a thing having feeding restraint action independent of Leptin became clear than an approved thing. Furthermore, by the continuance dosage in a cerebral ventricle of morpholino Nesfatin-lantisense, a feeding rose to obvious fact, and the weight rose linearly from the sixth day. These results were carried by Nature443 : 709-712, 2006.I did cloning of this Nesfatin/NUCB2 genome and built targeting vector which did decrease of Exon3-4by homologous recombination method and performed introduction to an embryonic stem cell, a selection, making of a chimera mouse step by step and was able to write back cross to 5 generations now.