Analysis and Clinical application of a novel satiety molecule stimulated by PPAR ganma knock out mouse

PPAR ganma敲除小鼠刺激的新型饱腹感分子分析及临床应用

• 批准号: 17390267
• 负责人: MORI Masatomo
• 金额: $ 9.86万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390267/
• 关键词: Nesfatin-1; Knockout mouse; Obesity; Nesfatin-1; Knockout mouse; Obesity; NAP-1; アデノウイルス; プロモーター

When a feeding was restrained for amount of medicine for usage dependence by the dosage in a rat brain of this re-combination Nantes protein and administered an anti nesfatin antibody, that I am significant, food consumption increased was recognized. It was recognized that there was a lot it in hypothalamus with connection for a feeding by immunity dyeing in a rat brain by an anti nesfatin antibody. I let a rat go without food for 24 hours, and, according to the experiment that I used NUCB2 cRNA for, the NUCB2 mRNA expression decreased with room side nucleus by fast distinctly. In addition, in the dosage in a cerebral ventricle of Nesfatin-1 peptide to the Zucker rat which there was Leptin acceptor abnormality, as for the feeding restraint action, as for Nesfatin-1, a thing having feeding restraint action independent of Leptin became clear than an approved thing. Furthermore, by the continuance dosage in a cerebral ventricle of morpholino Nesfatin-lantisense, a feeding rose to obvious fact, and the weight rose linearly from the sixth day. These results were carried by Nature443 : 709-712, 2006.I did cloning of this Nesfatin/NUCB2 genome and built targeting vector which did decrease of Exon3-4by homologous recombination method and performed introduction to an embryonic stem cell, a selection, making of a chimera mouse step by step and was able to write back cross to 5 generations now.

当通过这种重组南特蛋白在大鼠大脑中的剂量来限制喂养的药物用量以满足使用依赖时，并给予抗Nesfatin抗体，这是显著的，食物消耗增加被认识到。用抗Nesfatin抗体对大鼠脑内进行免疫染色，发现下丘脑中有大量的Nesfatin与摄食有关。我让大鼠禁食24小时，根据我用NUCB2CRNA进行的实验，NUCB2mRNA的表达随着房间侧核的快速而明显下降。此外，在脑室给有瘦素受体异常的Zucker大鼠脑室注射Nesfatin-1多肽的剂量中，对于摄食抑制作用，对于Nesfatin-1，有一种不依赖瘦素的摄食抑制作用变得比公认的东西更清楚。此外，脑室持续给药后，小鼠摄食明显增加，体重从第6天开始呈线性上升。本研究结果发表于《自然》杂志443：709-712,2006。我对Nesfatin/NUCB2基因组进行了克隆，构建了靶向载体，利用同源重组的方法对外显子3-4进行了缺失，并对胚胎干细胞进行了导入，对嵌合体小鼠进行了分步筛选、制作，现已能回写杂交至5代。

项目成果

Molecular mechanisms associated with leptin resistance: n-3 polyunsaturated fatty acids induce alterations in the tight junction of the brain

• DOI: 10.1016/j.cmet.2005.04.004
• 发表时间: 2005-05-01
• 期刊: CELL METABOLISM
• 影响因子: 29
• 作者: Oh-I, S;Shimizu, H;Mori, M
• 通讯作者: Mori, M

Isolation and characteriztion of a transcriptional cofactor and its novel isoform that bind the deoxyribonucleic acid-binding domein of peroxisome proliferators-activated receptor-γ.

转录辅助因子及其结合过氧化物酶体增殖物激活受体-γ 脱氧核糖核酸结合域的新型亚型的分离和表征。

• 发表时间: 2006
• 期刊: Endocrinology 144
• 作者: 日出山拓人;郭 伸;Nikrodhanond AA;Hashikoto K;Tomaru T
• 通讯作者: Tomaru T

Akt2 phosphorylates Synip to regulate docking and fusion of GLUT4 containing vesicles, Identification of Akt2 specific substrate.

Akt2 磷酸化 Synip 以调节含有 GLUT4 的囊泡的对接和融合，Akt2 特异性底物的鉴定。

• 发表时间: 2005
• 期刊: J Cell Biol 168
• 作者: 日出山拓人;郭 伸;Nikrodhanond AA;Hashikoto K;Tomaru T;Yamada M;Hashimoto K;Oh-I S;Nikrodhanond AA;Tomaru T;Hashimoto K;Oh-I S;Nikrodhanond AA;Hashikoto K;Hashimoto K;Oh-I S;Hashimoto K;Yamada E
• 通讯作者: Yamada E

nhibition of appetite by nasal leptin administration in rats.

鼻饲瘦素对大鼠食欲的抑制作用。

• 发表时间: 2005
• 期刊: Int J Obes Relat Metab Disord 29
• 作者: 日出山拓人;郭 伸;Nikrodhanond AA;Hashikoto K;Tomaru T;Yamada M;Hashimoto K;Oh-I S;Nikrodhanond AA;Tomaru T;Hashimoto K;Oh-I S;Nikrodhanond AA;Hashikoto K;Hashimoto K;Oh-I S;Hashimoto K;Yamada E;Shimizu H
• 通讯作者: Shimizu H

Cross-talk between thyroid hormone receptor and liver X receptor regulatory pathways is revealed in a thyroid hormone resistance mouse model

• DOI: 10.1074/jbc.m507877200
• 发表时间: 2006-01-06
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Hashimoto, K;Cohen, RN;Wondisford, FE
• 通讯作者: Wondisford, FE