Mechanisms determining morphology and dynamics of the mitochondrial inner membrane

决定线粒体内膜形态和动力学的机制

• 批准号: 459304237
• 负责人: Dr. Till Klecker
• 金额: --
• 依托单位: Arbeitsgruppe für Zellbiologie und Elektronenmikroskopie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/459304237?language=en
• 关键词: Mechanisms; determining; morphology; dynamics; mitochondrial

Mitochondria adopt a characteristic ultrastructure. They are surrounded by a double membrane and the inner membrane folds into invaginations that are called cristae. The structure of both mitochondrial membranes is furthermore influenced by recurring fusion and fission events. In this project, we plan to study the molecular mechanisms that determine the dynamics and morphology of the mitochondrial inner membrane in yeast. Some components that play central roles in these processes have already been identified. Important examples are the MICOS complex, the F1FO-ATP synthase, and the GTPase Mgm1. However, a complete picture of all the factors that help to maintain mitochondrial ultrastructure is still missing. Furthermore, it is currently unknown whether also higher eukaryotes possess a separate machinery for the division of the inner membrane, as it is the case in some protists and algae. Our preliminary results indicate that the inner membrane can be divided in the absence of outer membrane dynamics in yeast. We plan to employ a combination of genome-wide genetic screens and light and electron microscopy to identify the components that are involved in this process. Furthermore, we propose to use a novel high throughput electron microscopy screen to systematically identify genes that are required for mitochondrial ultrastructure maintenance. We are confident that the planned experiments will substantially contribute to our understanding of the mechanisms that determine the morphology and dynamics of the mitochondrial inner membrane.

线粒体采用特有的超微结构。它们被双层膜包围，内膜折叠成内陷，称为嵴。此外，线粒体膜的结构还受到反复发生的融合和裂变事件的影响。在这个项目中，我们计划研究决定酵母线粒体内膜动力学和形态的分子机制。在这些过程中发挥核心作用的一些组件已经被确定。重要的例子是 MICOS 复合体、F1FO-ATP 合酶和 GTPase Mgm1。然而，仍然缺乏有助于维持线粒体超微结构的所有因素的完整信息。此外，目前尚不清楚高等真核生物是否也拥有独立的内膜分裂机制，就像某些原生生物和藻类的情况一样。我们的初步结果表明，酵母的内膜可以在没有外膜动力学的情况下分裂。我们计划结合使用全基因组遗传筛选以及光学和电子显微镜来识别参与该过程的成分。此外，我们建议使用新型高通量电子显微镜屏幕来系统地识别线粒体超微结构维持所需的基因。我们相信，计划的实验将极大地有助于我们理解决定线粒体内膜形态和动力学的机制。