Molecular Epidemiological Surveillance of Equine Babesiosis in Japan

日本马巴贝斯虫病的分子流行病学监测

• 批准号: 11556056
• 负责人: FUJISAKI Kozo
• 金额: $ 8.64万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11556056/
• 关键词: Babesia caballi; B. equi; ELISA; Dermacentor; PCR; hemocyte; Haemaphysalis longicornis; ヘモサイト; ピロプラズマ原虫; バベシア・エクイ; バベシア・カバリ; 介卵伝播; 生物活性物質; taransmission blocking vaccine; バベシア原虫; ウエスタンブロット; ウマバベシア原虫; リコンビナント抗原; モノクロナール抗体; エライザ

(1) Enzyme-linked immunosorbent assay (ELISA), latex agglutination tests, and Western blot using recombinant proteins (EMA-1 and GST-BC48) have been established and their high sensitivity and specificity for antibody detection have been demonstrated using sera collected from horses of Brazil.(2) A serological survey of equine babesios in Japan was carried out on 2,019 sera collected during 1971-1973 by the above-mentioned ELISA and Western blot. These results suggested that the existence of equine babesiosis in Japan by the detection of antibodies against Babesia caballi and B. equi.(3) The PCR for amplification of specific-equine Babesia parasite gene fragments from vector ticks was established using Dermacentor ticks collected from the pasture of Mongolia.(4) The possible role of Haemaphysalis longicornis, the most prevalent tick in Japan, and its eggs in the transmission of Babesia caballi parasites was documented using the above-mentioned PCR.(5) The internal defense mechanisms of ticks against equine Babesia parasites, such as the hemocyte phagocytosis and bioactive molecules, were investigated in order to develop the novel transmission-blocking vaccines.

(1)建立了重组蛋白(EMA-1和GST-BC48)的酶联免疫吸附试验(ELISA)、胶乳凝集试验和Western印迹检测抗体的方法，并用巴西马的血清进行了抗体检测。(2)对日本1971-1973年采集的2,019份马巴贝斯虫进行了血清学调查。这些结果表明，通过检测马巴贝斯虫和马巴贝斯虫的抗体，日本存在马巴贝斯虫病。(3)利用从蒙古牧场采集的粉虱建立了从媒介硬蜱中扩增马巴贝斯虫寄生虫基因片段的PCR方法。(4)利用上述PCR技术，研究了日本最流行的长角血蜱及其卵在传播马巴贝斯虫寄生虫中的可能作用。(5)为了开发新型传播阻断疫苗，研究了硬蜱对马巴贝斯虫寄生虫的内部防御机制，如血细胞吞噬功能和生物活性分子。

项目成果

Battsetseg, B. et al.: "Diagnosis of Babesia cabalii and Babesia equi infections in Dermacentor nuttalli adult ticks"Int. J. Parasitol.. 31-4. 384-386 (2001)

Battsetseg，B. 等人：“Dermacentor nuttalli 成年蜱中的巴贝斯虫和马巴贝斯虫感染的诊断”Int。

Inoue,N.: "Characterization of phagocytic hemocytes in Ornithodoros moubata (Acari : Ixodidae)."Journal of Medical Entomology. 38(印刷中). (2001)

Inoue, N.：“Ornithodoros moubata（蜱虫科）吞噬细胞的特征”，《医学昆虫学杂志》38（出版中）。

Lnoue, N.: "Characterization of phagocytic hemocytes in Ornithodoros moubata (Acari Ixodidae)"J. Med. Entomol. 38・4. 514-519 (2001)

Lnoue, N.：“毛白蜱虫吞噬血细胞的特征”J. Med. 514-519 (2001)。

Fujisaki, K.: "Technical Manual for the Examination and Control of Parasites of Domestic Animals"Japan Livestock Technology Association, Tokyo. 236 (2001)

Fujisaki, K.：“家畜寄生虫检查和控制技术手册”，日本畜牧技术协会，东京。

Ikadai, H.: "Analysis of a growth-promoting factor for Babesia caballi cultivation"J. Parasitol. 87(6): 1484-1486. 87・6. 1484-1486 (2001)

Ikadai, H.：“巴贝虫栽培的生长促进因子的分析”J. Parasitol. 87(6): 1484-1486 (2001)。