Molecular Epidemiological Surveillance of Equine Babesiosis in Japan

日本马巴贝斯虫病的分子流行病学监测

• 批准号: 11556056
• 负责人: FUJISAKI Kozo
• 金额: $ 8.64万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11556056/
• 关键词: Babesia caballi; B. equi; ELISA; Dermacentor; PCR; hemocyte; Haemaphysalis longicornis; ヘモサイト; ピロプラズマ原虫; バベシア・エクイ; バベシア・カバリ; 介卵伝播; 生物活性物質; taransmission blocking vaccine; バベシア原虫; ウエスタンブロット; ウマバベシア原虫; リコンビナント抗原; モノクロナール抗体; エライザ

(1) Enzyme-linked immunosorbent assay (ELISA), latex agglutination tests, and Western blot using recombinant proteins (EMA-1 and GST-BC48) have been established and their high sensitivity and specificity for antibody detection have been demonstrated using sera collected from horses of Brazil.(2) A serological survey of equine babesios in Japan was carried out on 2,019 sera collected during 1971-1973 by the above-mentioned ELISA and Western blot. These results suggested that the existence of equine babesiosis in Japan by the detection of antibodies against Babesia caballi and B. equi.(3) The PCR for amplification of specific-equine Babesia parasite gene fragments from vector ticks was established using Dermacentor ticks collected from the pasture of Mongolia.(4) The possible role of Haemaphysalis longicornis, the most prevalent tick in Japan, and its eggs in the transmission of Babesia caballi parasites was documented using the above-mentioned PCR.(5) The internal defense mechanisms of ticks against equine Babesia parasites, such as the hemocyte phagocytosis and bioactive molecules, were investigated in order to develop the novel transmission-blocking vaccines.

（1）已经建立了使用重组蛋白（EMA-1和GST-BC48）的酶连接免疫吸附测定（ELISA），乳胶凝集测试和蛋白质印迹，并使用Sera在BARAZIL上进行了2份抗体的抗体检测证明了它们的高灵敏度和特异性。上述ELISA和Western印迹期间在1971 - 1973年收集的血清。这些结果表明，日本的降铁在日本的存在是通过发现针对Babesia caballi和B. Equi的抗体。（3）在使用以上风的tick虫中收集的大多数tick虫（4）蒙古人的作用（4）tick虫（4）HAYEMASH of HAYEMACHIS，HAYEMASK tick tick ticks ticks ticks ticks ticks ticks ticks ticks ticks ticks ticks ticks ticks的pCR（4）。 （5）（5）针对马巴比西亚寄生虫的tick虫的内部防御机制（例如血细胞吞噬作用和生物活性分子），研究了其内部防御机制，以开发新的传播疫苗。

项目成果

Inoue, N.: "Characterization of phagocytic hemocytes in Ornithodoros moubata (Acari: Ixodidae)"J. Med. Entomol.. 38・4. 514-519 (2001)

Inoue, N.：“Onithodoros moubata（蜱虫：蜱科）吞噬细胞的特征”J. Med. Entomol.. 514-519 (2001)。

Battsetseg, B. et al.: "Natural infection of Boophilus microplus with Babesia equi and Babesia caballi in Brazilian horses and evidence or transovarial transmission using nested polymerase chain reaction"Vet. Parasitol.. (in press). (2002)

Battsetseg, B. 等人：“巴西马体内马巴贝斯虫和马巴贝斯虫对微小牛杆菌的自然感染以及使用巢式聚合酶链式反应进行跨卵巢传播的证据”兽医。

Inoue,N.: "Characterization of phagocytic hemocytes in Ornithodoros moubata (Acari : Ixodidae)."Journal of Medical Entomology. 38(印刷中). (2001)

Inoue, N.：“Ornithodoros moubata（蜱虫科）吞噬细胞的特征”，《医学昆虫学杂志》38（出版中）。

Battsetseg, B. et al.: "Diagnosis of Babesia cabalii and Babesia equi infections in Dermacentor nuttalli adult ticks"Int. J. Parasitol.. 31-4. 384-386 (2001)

Battsetseg，B. 等人：“Dermacentor nuttalli 成年蜱中的巴贝斯虫和马巴贝斯虫感染的诊断”Int。

Lnoue, N.: "Characterization of phagocytic hemocytes in Ornithodoros moubata (Acari Ixodidae)"J. Med. Entomol. 38・4. 514-519 (2001)

Lnoue, N.：“毛白蜱虫吞噬血细胞的特征”J. Med. 514-519 (2001)。