Usefulness of APIMS for monitoring of nitrogen dioxides during NO inhaling therapy

APIMS 在 NO 吸入治疗期间监测二氧化氮的有用性

• 批准号: 11557114
• 负责人: KINOUE Takaaki
• 金额: $ 5.7万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11557114/
• 关键词: nitric oxide; nitrogen dioxide; APIMS; NO inhaling therapy; pulmonary hypertension

This project was conducted for measuring directly nitrogen dioxide during nitric oxide inhaling therapy. An Atmospheric Pressure Ionization Mass Spectrometry (APIMS) device was utilized in this study. First we improved the prototype of APIMS by changing the detector and the slit sizes between the first ionization room and the second ionization room. Then combinations of ionization gas and sample carrier gas were investigated. We found the good combination of SF6 in Ar for ionization gas and Ar for sample carrier gas for determining NO2. Calibration curve of NO2 in NO was obtained successfully, which was impossible by conventional chemiluminescence method detectors. Chemiluminescence analyzer and this system was applied for evaluation of the quality of absorber of impurity gases in pure gas. Molecular Sieve was the best absorber for removal of NO2 in NO.Moreover if SODALIME was used for absorber before entering into the patient, we noticed that the definite necessity of good filter (filter may produce any impurities) in order to remove small particulate matter or many kinds of impurities, because of APIMS showed many irregular peaks in the spectrogram. Then we applied nitrogen oxides in the concentration of less than 1 % of nitric oxide during NO inhaling therapy for model animals of pulmonary hypertension by monocrotaline. This animal model was not so sensitive for NO therapy itself that we could not see effects of varied concentration of nitrogen dioxides in NO for longer duration of therapy. Other methods for mimic pulmonary hypertension such as hypoxia should be tried and longer period observation of the conditions of animals up to several hours must be enabled.

本计画系针对一氧化氮吸入疗法中二氧化氮之直接量测。在这项研究中使用了大气压电离质谱（APIMS）装置。首先，我们通过改变探测器和第一电离室和第二电离室之间的狭缝尺寸来改进APIMS的原型。然后研究了电离气体和样品载气的组合。我们发现，在Ar中的SF6作为电离气体和Ar作为样品载气的良好组合用于测定NO2。成功地得到了NO中NO2的标准曲线，这是常规化学发光法检测器无法实现的。应用化学发光分析仪和该系统对纯气中杂质气体吸收剂的质量进行了评价。分子筛是去除NO中NO2的最佳吸收剂。此外，如果在进入患者体内之前使用SODALIME作为吸收剂，我们注意到，为了去除小颗粒物质或多种杂质，必须使用良好的过滤器（过滤器可能产生任何杂质），因为APIMS在光谱图中显示出许多不规则峰。然后在吸入NO治疗野百合碱肺动脉高压模型动物的过程中，应用浓度小于1%的一氧化氮。该动物模型对NO治疗本身不太敏感，以至于我们无法看到NO中不同浓度的二氧化氮对更长治疗时间的影响。应尝试模拟肺动脉高压的其他方法，如缺氧，并必须能够对动物的状况进行长达数小时的长期观察。

项目成果

Takaaki Kinoue 他: "Direct determination of trace nitrogen dioxide by Atmospheric Pressure Ionization Mass Spectrometry(APIMS)without conversion to nitric oxide."Environmental Health and Preventive Medicine. 5・3. 97-102 (200)

Takaaki Kinoue 等人：“通过大气压电离质谱 (APIMS) 直接测定痕量二氧化氮，无需转化为一氧化氮。”环境健康与预防医学 5・3 (200)。

Takaaki Kinoue, Satoshi Asai, Yukimoto Ishii, Koichi Ishikawa, Masashi Fujii, Kazuo Nakano, and Keiji Hasumi: "Direct determination of trace nitrogen dioxide by Atmospheric Pressure Ionization Mass Spectrometry (APIMS) without conversion to nitric oxide."

Takaaki Kinoue、Satoshi Asai、Yukimoto Ishii、Koichi Ishikawa、Masashi Fujii、Kazuo Nakano 和 Keiji Hasumi：“通过大气压电离质谱 (APIMS) 直接测定痕量二氧化氮，无需转化为一氧化氮。”

Takaaki Kinoue 他: "Direct determination of trace nitrogen dioxide by Atmospheric Pressure Ionization Mass Spectrometry (APIMS) without conversion to nitric oxide."Environmental Health and Preventive Medicine. 5・3. 97-102 (2000)

Takaaki Kinoue 等人：“通过大气压电离质谱 (APIMS) 直接测定痕量二氧化氮，无需转化为一氧化氮。”环境健康与预防医学 5・3 (2000)。