Prevention and treatment of periodntal disease ; the developments of mucosal vaccination and protease inhibitor

牙周病的预防和治疗；

基本信息

批准号：
11557133
负责人：
UMEMOTO Toshio
金额：
$ 8.64万
依托单位：
Kanagawa Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

This study deals with local and systemic immune responses to Porphyromonas gingivalis ATCC 33277 fimbriae after mucosal vaccination of mice via intranasal route with the fimbriae and recombinant cholera toxin B subunit (rCTB). The fimbriae by itself stimulated systemic immune responses even at a low dose (0.5 μg), and serum IgG and IgA specific to P. gingivalis fimbriae were induced in an antigen dose-dependent manner. Induction of the serum IgG and IgA was started from 10 days after the first immunization, and the levels continued to elevate until sacrifice day. rCTB co-administration did not enhance the levels of systemic responses, but the levels of serum IgA were slightly increased by rCTB-adjuvant effect when the mice was immunized with 0.5 μg of the fimbriae. The serum IgG subclass titers were revealed to be the order of IgG1 > IgG3 > IgG2b > IgG2a, suggesting that Th1 and Th2 type immune systems were stimulated by this immunization. In addition to systemic response, mucosal vacc … More ination with P. gingivalis fimbriae more than 5 μg also stimulated mucosal IgA response which was started to elevated from 18 days after the first immunization. The mucosal IgA response was significantly enhanced by rCTB co-administration, and in particular, secretory IgA specific to P. gingivalis fimbriae was induced into saliva, nosal cavity and lung at a high level even at a low dose (0.5 μg fimbriae). Thus, the mucosal vaccination with co-administration of P. ginigvalis fimbriae and rCTB via intranasal route strongly stimulated not only systemic immune response but also mucosal response, indicating that this vaccination may be efficacious for the prevention of P. gingivalis-mediated periodontal disease.In addition, an approximately 80 % reduction of P. gingivalis-mediated alveolar bone resorption in mice was induced by nasal administration of the fimbrial vaccine.Thus, nasal administration of the vaccine containing fimbriae and rCTB strongly stimulated both systemic and mucosal responses and may be effective for the prevention of P. gingivalis-mediated periodontitis. Less

这项研究涉及局部和全身免疫反应对小鼠粘膜疫苗在小鼠粘膜疫苗通过鼻内途径的粘膜和重组霍乱毒素B亚基（RCTB）的粘膜疫苗（RCTB）。即使在低剂量（0.5μg）下，叶片本身也刺激了全身免疫反应，并且以抗原剂量依赖性方式诱导了对牙龈斑ap虫纤维化膜的血清IgG和IgA。血清IgG和IgA的诱导是从第一次免疫抑制后的10天开始的，水平继续升高到牺牲日。 RCTB共同给药并不能提高全身反应的水平，但是当小鼠用0.5μg的纤维膜免疫时，RCTB-Adjuvant效应略微增加了血清IgA的水平。血清IgG亚类滴度显示为IgG1> IgG3> IgG2B> IgG2A的顺序，这表明TH1和TH2型免疫系统受到这种免疫配置的刺激。除了系统性反应外，粘膜Vac…与牙龈疟原虫纤维化膜的更多相互关系超过5μg，还刺激了粘膜IGA反应，从第一次免疫配置后的18天开始，该反应开始升高。 RCTB共同给药显着增强了粘膜IgA反应，尤其是，即使在低剂量下（0.5μgfimbriae），也可以将特定于牙龈牙龈牙龈利疟原虫的秘密IgA诱导到唾液，nosal腔和肺中。这是，通过鼻内途径与ginigvalis fimbriae和rctb共同给药的粘膜疫苗不仅强烈刺激不仅是全身免疫反应，而且还强烈刺激了粘膜反应，而且粘膜反应也可以有效，表明该疫苗可以有效地预防牙龈杂志介导的周期性疾病。通过鼻施用纤维疫苗。因此，含有fimbriae和RCTB的疫苗的鼻施用强烈刺激了全身和粘膜反应，并且可能有效预防牙龈疟原虫介导的牙周炎。