The development of insulin resistance syndrome model rats using fast-twitch fiber dominant rats

利用快肌纤维优势大鼠建立胰岛素抵抗综合征模型

• 批准号: 11558002
• 负责人: KUMAGAI Shuzo
• 金额: $ 6.34万
• 依托单位: KYUSHU UNIVERSTIY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11558002/
• 关键词: muscle fiber composition; fast twitch fiber dominant rat; insulin sensitivity; insulin resistance; insulin responsiveness; energy metabolism; enzyme activity; wheel-running; 筋線維組成; 速筋線維優位ラット; FFDR; 糖取り込み; 高脂肪食

There are several reports that the muscle fiber composition is associated with body fat accumulation and insulin silmulated glucose uptake. It was considered that higher percentage of fast-twitch type II fibers was one of the determinants of obesity and insulin resistance syndrome, However, the causality between muscle fiber composition and such syndrome was still unclear. We previously produced the fast-twitch fibers dominant rats (FFDR) obtained by the selection breeding for high percentage of fast-twitch type II fibers and control rats (CR) obtained by the random breeding, Using FFDR and CR, we examined whether genetically difference of muscle fiber composition affected the dietary obesity proneness by high-fat diet, In addition, we examined whether the insulin silmulated glucose uptake in FFDR differed the CR by hyperinsulinemic euglycemic clamp technique. Furthermore, voluntary wheel-running activity level in FFDR was also determined and compared with the CR. The FFDR showed more obesity-resistant and higher muscle oxidative enzyme activity than CR, Moreover, FFDR indicated higher insulin responsiveness than CR. In addition, the FFDR showed higher physical activity level than CR. Based on these results, we propose that the muscle metabolic characteristic rather than muscle fiber composition is a possible determinant of obesity and insulin resistance syndrome, Furthermore, such animal group might be a good model for the association between insulin sensitivity and muscle characteristics.

有几份报告指出，肌纤维组成与体脂积累和胰岛素模拟葡萄糖摄取有关。高比例的快缩II型肌纤维被认为是肥胖和胰岛素抵抗综合征的决定因素之一，但肌纤维组成与肥胖和胰岛素抵抗综合征的因果关系尚不清楚。本研究通过选择性饲养获得的FFDR和随机饲养获得的对照组大鼠，利用FFDR和对照组大鼠，研究了肌纤维组成的遗传差异对高脂饮食引起的肥胖倾向的影响。我们通过高胰岛素正葡萄糖钳夹技术检测了FFDR中胰岛素模拟的葡萄糖摄取是否与CR不同。此外，还确定了FFDR中的自愿轮跑活动水平，并与CR进行了比较。FFDR组比CR组具有更好的抗肥胖性和更高的肌肉氧化酶活性，并且FFDR组比CR组具有更高的胰岛素反应性。此外，FFDR显示出比CR更高的体力活动水平。基于这些结果，我们提出肌肉代谢特性而不是肌纤维组成可能是肥胖和胰岛素抵抗综合征的决定因素，并且，这种动物组可能是胰岛素敏感性与肌肉特性之间关联的良好模型。

项目成果

熊谷秋三他: "インスリン抵抗性と骨格筋の組織化学的・生化学的特性との関連性"運動生理学雑誌. 6. 1-16 (1999)

Akizo Kumagai 等人：“胰岛素抵抗与骨骼肌组织化学和生化特性之间的关系”运动生理学杂志 6. 1-16 (1999)。

諏訪雅貴,熊谷秋三,佐々木悠: "生活習慣病予防と運動による健康支援-骨格筋特性との関連から-"健康支援. 第2巻 第1・2号. 1-11 (2000)

Masataka Suwa、Akizo Kumagai、Yu Sasaki：“通过运动预防生活方式疾病和健康支持 - 从与骨骼肌特征的关系 -”健康支持第 2 卷第 1 和 2. 1-11 (2000)。

Suwa M, Kumagai S, Sasaki H: "Health promotion according to exercise and physical activity and prevention of lifestyle-related disease"Japanese Journal of Health Promotion. 2・1. 1-11 (2000)

Suwa M、Kumagai S、Sasaki H：“根据运动和体力活动促进健康以及预防生活方式相关疾病”日本健康促进杂志2·1（2000）。

Suwa M, Kumagai S, Higaki Y, Nakamura T, Katsuta S: "Dietary obesity-resistance and muscle oxidative enzyme activities of the fast-twitch fibre dominant rat"International Journal of Obesity. (Accepted for publication).

Suwa M、Kumagai S、Higaki Y、Nakamura T、Katsuta S：“快肌纤维优势大鼠的饮食肥胖抵抗和肌肉氧化酶活性”国际肥胖杂志。

Suwa M, Kumagai S, Higaki Y, Nakamura T, Katsuta S: "Dietary obesity-resistance and muscle oxidative enzyme activities of the fast-twitch fiber dominant rats"International Journal of Obesity. (in press).

Suwa M、Kumagai S、Higaki Y、Nakamura T、Katsuta S：“快肌纤维优势大鼠的饮食肥胖抵抗和肌肉氧化酶活性”国际肥胖杂志。