Use of mitochondrial electron transport chain mutants to evaluate how non-phosphorylating respiration influences plant metabolite profiles and stress tolerance.

本项目利用转基因植物技术阐明线粒体功能如何影响植物细胞和组织中代谢物的谱，以及这些谱的改变是否会影响植物在恶劣条件下的生长能力。利用植物胁迫反应的代谢组学分析将有助于我们对植物代谢的基础知识。这将使用新的高通量技术来实现，允许对大量样品进行可靠的定性和定量分析。这种方法将补充现有的植物暴露于非生物胁迫的基因组和蛋白质组学分析。

This project uses transgenic plant technology to elucidate how mitochondrial function impacts on the profile of metabolites in plant cell and tissues and whether altering these profiles influences a plant's ability tog row in harsh conditions. It will contribute to our fundamental knowledge of plant metabolism using a metabolomic anaylsis of plant stress response. This will be achieved using new high-throughput technologies, allowing reliable qualitative and quantitative analysis of large numbers of samples. This approach will compliment existing genomic and proteomic analyses of plants exposed to abiotic stress.