Establishment of novel techniques for manipulating mammalian fetuses at the molecular level
建立分子水平操纵哺乳动物胎儿的新技术
基本信息
- 批准号:11558090
- 负责人:
- 金额:$ 8.64万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B).
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Brain functions such as memory, lerning, and emotion, are based on neuronal networks connected among various neurons in specific regions. In order to elucidate molecular mechanisms of brain development, it is required to establish novel techniques to directly manipulate mammalian fetuses of the middle gestation and thereafter at molecular levels. This research project aimed to do the following subjects :1) Establishment of techniques for manipulating and maintaining mammalian fetuses at the middle gestation and thereafter2) Establishment of techniques for transplanting cells into the brain of mammalian fetuses at the middle gestation and thereafter3) Establishment of techniques for transferring exogenous genes into the brain of mammalian fetuses at the middle gestation and thereafterThe following results are obtained in the period supported by this grant :1) As a technique for manipulating and maintaining mammalian fetuses at the middle gestation and thereafter, we established ultrasound-guided in utero manipulation, thereby operated fetuses being able to survive and develop at the full term.2) As preliminary experiments for cell transplantation and gene transfer, we tried to inject dye solutions into the operated fetuses.
大脑的功能,如记忆、学习和情绪,是基于特定区域内不同神经元之间连接的神经元网络。为了阐明大脑发育的分子机制,需要建立新的技术来直接操纵怀孕中期的哺乳动物胎儿,并在分子水平上进行操作。本研究项目的目的是:1)建立妊娠中期及以后哺乳动物胎儿的操作和维持技术2)建立中期和之后哺乳动物胎儿脑内移植细胞的技术3)建立在中期和之后将外源基因转移到哺乳动物胎儿脑内的技术。在这项资助的支持期间,我们获得了以下结果:1)作为一种在中期和此后操作和维持哺乳动物胎儿的技术,我们建立了超声引导下的宫内操作,从而使手术后的胎儿能够存活和发育到足月,2)作为细胞移植和基因转移的初步实验,我们试图将染料溶液注入手术后的胎儿体内。
项目成果
期刊论文数量(52)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nagase,T.,Shimoda,Y.,Sanai,Y.,Nakamura,S.,Harii,K.,Osumi,N.: "Differential Expression of Two Glucuronyltransferases Synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors."Mechanisms of Development. 98. 145-149 (2000)
Nagase,T.,Shimoda,Y.,Sanai,Y.,Nakamura,S.,Harii,K.,Osumi,N.:“在大鼠肌源祖细胞亚系中合成 HNK-1 碳水化合物表位的两种葡萄糖醛酸基转移酶的差异表达
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Yamamoto, H., Ochiya, T., Takahama, Y., Ishii, Y., Osumi, N., Sakamoto, H., Terada, M.: "Detection of spatial localization of Hst-Fgf-4 gene expression in brain and testis from adult mice."Oncogene. 19. 3805-3810 (2000)
Yamamoto, H.、Ochiya, T.、Takahama, Y.、Ishii, Y.、Osumi, N.、Sakamoto, H.、Terada, M.:“大脑中 Hst-Fgf-4 基因表达的空间定位检测
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Kawano, H., Fukuda, T., Kubo, K., Horie, M., Takeuchi, K., Osumi, N., Eto, K., and Kawamura, K.: "Pax6 is required for the thalamocortical pathway formation in fetal rats."J.Comp.Neurol.. 408. 147-160 (1999)
Kawano, H.、Fukuda, T.、Kubo, K.、Horie, M.、Takeuchi, K.、Osumi, N.、Eto, K. 和 Kawamura, K.:“Pax6 是丘脑皮质通路形成所必需的
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Suzuki.R.,Shintani.T.,Sakuta,H.,Kato,A,Ohkawara,T.,Osumi,N.,. et al.: "Identification of RALDH-3, a novel retinaldehyde dehydrogenase, expressed in the ventral region of the retina."Mechanisms of Development. 98. 37-50 (2000)
铃木.R.,新谷.T.,咲田,H.,加藤,A,大河原,T.,大隅,N.,。
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Torii,M.,Matsuzaki,F.,Osumi,N.,et al.: "Transcription factors Mash-1 and Prox-1 delineate early steps in differentiation of neural stem cells in the developing central nervous system"Development. 126. 443-456 (1999)
Torii,M.、Matsuzaki,F.、Osumi,N.等人:“转录因子 Mash-1 和 Prox-1 描绘了发育中的中枢神经系统中神经干细胞分化的早期步骤”的开发。
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{{ truncateString('OSUMI Noriko', 18)}}的其他基金
Epigenetic changes of male germ line cells affecting behavior of offspring in mice
雄性生殖系细胞的表观遗传变化影响小鼠后代的行为
- 批准号:
15K12764 - 财政年份:2015
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
The role of RNA-binding protein for long-distance transportation of mRNA within embryonic neural progenitor cells
RNA结合蛋白在胚胎神经祖细胞内长距离运输mRNA中的作用
- 批准号:
26291046 - 财政年份:2014
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis on the neural basis for vocal communication in the mouse
小鼠声音交流的神经基础分析
- 批准号:
25640002 - 财政年份:2013
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Participation of the oligodendrocyte/oligodendrocyte precursor cell in the morbidity of schizophrenia
少突胶质细胞/少突胶质细胞前体细胞参与精神分裂症的发病
- 批准号:
23650186 - 财政年份:2011
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Roles of fatty acids and fatty acid binding proteins for regulating proliferation and differentiation of neural stem cells
脂肪酸和脂肪酸结合蛋白在调节神经干细胞增殖和分化中的作用
- 批准号:
21300115 - 财政年份:2009
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study on molecular mechanisms for regulating proliferation and differentiation of embryonic neural stem cells
调控胚胎神经干细胞增殖分化的分子机制研究
- 批准号:
17024001 - 财政年份:2005
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
The role of Pax6 gene in vertebrate neural development
Pax6基因在脊椎动物神经发育中的作用
- 批准号:
11470002 - 财政年份:1999
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Abnormal Craniofacial Morphogenesis of the Small Eye Rat
小眼大鼠颅面形态发生异常
- 批准号:
08457475 - 财政年份:1996
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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