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Development of a System Recording Neural Activity from Moving Animals

记录运动动物神经活动的系统的开发

基本信息

批准号：
11558095
负责人：
SASAKI Shigeto
金额：
$ 6.98万
依托单位：
Tokyo Metropolitan Institute for Neuroscience
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11558095/
关键词：
Petarode Multiple Unit Spike sorting Unit Recording Moving animal Spinal cord Electrode array スパイクソーター

项目摘要

A major aim of this study is to develop a system that allow simultaneous recording of unit activities from multiple sites in the brain or spinal cord of moving animals.1. Development of pentarode We developed a tungusten electrode (500 mum in diameter) in which five thin cupper or silver wires of 20-50 mum thick are aranged along its long axis upto the tip of the electrode. Simultaneous Recoding from 5 diffent foci of the electrode can be made by removing the insulation of thin wires locally (pentarode).2. Development of a method recording from spinal cord We developed a method recording unit activities from the spinal cord in moving animals. We placed teflon-coated thin silber wires, embeded in a glass microelectrode of 2-3 mm length into the spinal cord and glued to the spinal cord.3. Development of a small maniplator We developed a small micro-maniplater (20 x 6 x 11 mm) which allows movements up to 15 mm in one direction. An array of 12 electorodes could be monted on it and could be implanted on the head of animals chronically.4. Development of a system analysing correlation between neural activitiesand behavior We developed a system correlating unit activities with various parameters of movements using PC-Unix (Linux), after sorting spikes by a template matching technique.

本研究的一个主要目的是开发一个系统，允许同时记录移动动物大脑或脊髓中多个部位的单位活动。我们开发了一种钨电极（直径500 μ m），其中五根20-50 μ m厚的细铜线或银沿其长轴沿着排列直到电极的尖端。通过局部去除细线的绝缘层（五极线），可以实现从电极的5个不同焦点的同时覆盖.脊髓电活动记录方法的建立我们建立了一种运动动物脊髓电活动记录方法。我们将聚四氟乙烯涂层的细银线，嵌入2-3 mm长的玻璃微电极中，并将其粘在脊髓中。我们开发了一种小型微型操纵器（20 x 6 x 11 mm），允许在一个方向上移动高达15 mm。可在其上安装12个电极阵列，并可长期植入动物头部。神经活动与行为相关性分析系统的开发我们在PC-Unix（Linux）上开发了一个将单位活动与各种运动参数相关联的系统，通过模板匹配技术对尖峰进行分类。