Phylogeny of Brain/Pituitary Hormones of Agnathans

无颌动物脑/垂体激素的系统发育

• 批准号: 11691132
• 负责人: KAWAUCHI Hiroshi
• 金额: $ 2.82万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11691132/
• 关键词: lamprey; proopiocortin; proopiomelanotropin; growth hormone; insulin-like growth factor; pituitary; gonadotropin; prolactin-releasing peptide; ウミヤツメ; ミナミヤツメ; フクロヤツメ; 遺伝子ライブラリー; 副腎皮質刺激ホルモン遺伝子; メラノトロビン遺伝子; 成長ホルモンcDNA; インスリン様成長因子cDNA; 生殖腺刺激ホル

Lampreys of the class Agnatha are of particular importance in understanding endocrinological relationships since they represent the oldest lineages of extant vertebrates. This project aimed to investigate the endocrine mechanisms of reproduction, growth and adaptation of the sea lampreys.I.The tissues including brains, pituitaries, and livers were collected from 10,000 adult female sea lampreys (Petromizon marinus) in New Hampshire and Michigan and used for the following studies(1) Isolation of prolactin-releasing peptide front brain(2) Isolation of growth hormone and cloning of its cDNA.(3) Determination of exon-intron structure of proopiocortin and proopiomelanotropin genes(4) Cloning of insulin-like growth factor cDNA from the liver.(5) Search for gonadotropin and its cDNA from the pituitary glands.(6) Ontogenic study on hormone-producing cells in the pituitary glands.II.200 juveniles of Modecia mordax and Geotoria australis were collected in rivers in Tasmania Island of Australia.Genome libraries of these lampreys are under construction.III.Organized the International symposium on 'Agnathan and Chondrichthyes Reproduction and Adaptation' at the 4th International Symposium on Fish Endocrinology, held in Seattle on 1 and 2 August, 2000 to not only update the status of agnathan endocrinology but also to extend the information to the Chondrichthyes.

无脊椎动物纲的七鳃鳗在理解内分泌关系方面特别重要，因为它们代表现存脊椎动物中最古老的谱系。本项目旨在研究七鳃鳗繁殖、生长和适应的内分泌机制。从新汉普郡和密歇根州采集了10,000尾成年雌性海鳗(Petromizon Marinus)的肝脏，用于以下研究：(1)催乳素释放肽前脑的分离(2)生长激素的分离及其基因的克隆(3)前阿片皮质素和前阿片黑色素基因外显子-内含子结构的测定(4)肝脏胰岛素样生长因子基因的克隆(5)垂体腺促性腺激素及其基因的寻找(6)垂体腺激素产生细胞的个体遗传学研究澳大利亚塔斯马尼亚岛上的河流。这些七鳃鳗的基因组文库正在建设中。III.在第四届国际鱼类内分泌学研讨会上组织召开了国际研讨会，主题是Agnathan和软骨鱼的繁殖和适应，2000年8月1日和2日在西雅图举行的会议，不仅更新了软骨内分泌学的最新状况，而且还将信息扩展到软骨鱼类。

项目成果

Nozaki,M. 他4名: "Adenohypophysial Cell Types in the Lamprey Pituitary : Current State of the Art."Comp.Biochem.Physiol.. (印刷中). (2001)

Nozaki, M. 和其他 4 人：“七鳃鳗垂体中的腺垂体细胞类型：当前的技术水平”Comp.Biochem.Physiol..（出版中）。

Moriyama, S., Furuta, M., Itch, T., Amemiya, Y., Takahashi, A., Kawauchi, H., Sower, S.A.and Hirano, T.: "Identification of prolactin-Releasing Peptide from Salmon and Lamprey."Proceeding of the Japan Society for Comparative Endocrinology. (Sasayama, Y.,

Moriyama, S.、Furuta, M.、Itch, T.、Amemiya, Y.、Takahashi, A.、Kawauchi, H.、Sower, S.A. 和 Hirano, T.：“来自鲑鱼和七鳃鳗的催乳素释放肽的鉴定

Komatsu, H., Amemiya, Y., Takahashi, A., Sower, S.A.and Kawauchi, H.: "Structure and expression of two proopiomelanocortin genes in lamprey."Proceeding of the Japan Society for Comparative Endocrinology. (Sasayama, Y., Suzuki, N.eds.)No.15. 54 (2000)

Komatsu, H.、Amemiya, Y.、Takahashi, A.、Sower, S.A. 和 Kawauchi, H.：“七鳃鳗中两个阿片黑皮素原基因的结构和表达”。日本比较内分泌学会会刊。

Oshima, Y., Nozaki, M.and Sower, S.A.: "Detection of immunoreactive cell types in the hagfish adenohypophysis.(Sasayama, Y., Suzuki, N.eds.)"Proceedings of the Japan Society for Comparative Endocrinology. No.15. 53 (2000)

Oshima, Y.、Nozaki, M. 和 Sower, S.A.：“盲鳗腺垂体中免疫反应细胞类型的检测。（Sasayama, Y.、Suzuki, N.eds.）”日本比较内分泌学会会刊。

Takahashi,A.,Amemiya,Y,Nozaki,M.,Sower,S.A.Kawauchi,H.: "Evolutionary signilicance of proopiomelanocortin in agnatha and chondrichthyes."Comp.Biochem.Biophys.. (印刷中). (2001)

Takahashi, A.、Amemiya, Y.、Nozaki, M.、Sower, S.A. Kawauchi, H.：“阿片黑皮素原在无颌类和软骨鱼中的进化意义”。Comp.Biochem.Biophys..（出版中）。