System for Screening for microorganisms with specific functions or specific microbial products

这个项目是在一个研究项目方案中完成的，作为大学机构之间的国际合作，有正式的交流方案协议。这项研究是为了开发筛选具有特定细胞功能或特定产物的生物体，而不考虑微生物、植物和动物。采用双方正在进行的研究作为模型系统。细胞术是研究的主要方法。由奈良研究所（NAIST）小组开发的“细胞分选显微镜系统”及其技术被用于单细胞藻类雨淋嗜血球菌生产虾青素和面包酵母生产硫胺素。最新的荧光原位杂交（FISH）技术从明尼苏达大学（UMN）组转移到NAIST组，以及凝胶微滴技术，用于流式细胞术中进行活细胞分选。实际上，工作人员和学生是从一个地方被派到另一个地方。在逗留期间举行了小型研讨会。学生们在实验室里住了几个星期，因为1999年和2001年是NAIST派代表去的。2000年，UMN派代表在田纳西大学教授的陪同下前往MAIST参加联合研讨会。大阪大学和广岛大学的教授也应邀出席。代表团的四名学生在国家科学技术研究院的实验室里呆了三个星期。在SRIENC教授的帮助下，两篇关于单细胞分析和细胞分选方法的综述论文发表在两份主要的国际期刊上。每个项目的成果报告也在编制中。

This project was done in a research projection programme as an international cooperation between university institutions with official agreement for exchange programme. The research was for development of screening of organisms with specific cell functions or specific products, regardless of microorganisms, plants, and animals. On-going researches on the both sides were used as the model systems. Cytometry was the main methodology for the researches. "Cell sorting microscope system" and its technology, developed by the Nara Institute (NAIST) group, were used tor the astaxanthm production with a unicellular alga Heamatococcus pluvialis and thiamin production with the bakers' yeast Saccharomyces cerevisiae. An up-to-date fluorescence in situ hybridisation (FISH) technique was transferred from the University of Minnesota (UMN) group to the NAIST group, as well as gel microdrdplet technique, to be used in flow cytometry for viable cell sorting.Practically, staff and students were sent from a part to the other part. There were small sminars during the stays. The students stayed for a few weeks in laboratories as the residents, lie years 1999 and 2001 were forthe NAIST to send the delegates. In 2000, the UMN sent the delegate accompanied by a professor from the University ofTennessee to the MAIST for the joint symposium. Professors from Osaka arid Hiroshima Universities Were also invited. The four students from the delegate stayed for three weeks in the laboratories at NAIST.With help of Professor SRIENC, two review papers on single-cell analysis and cell sorting methodologies were presented in two major international journals. Report of the results from every project were also in preparation.