System for Screening for microorganisms with specific functions or specific microbial products

该项目是在一个研究预测方案中完成的，作为大学机构之间的国际合作，有正式的交流方案协议。该研究旨在开发具有特定细胞功能或特定产品的生物体的筛选，无论微生物，植物和动物。模型系统采用双方正在进行的研究。细胞计数法是主要的研究方法。利用奈良研究所（NAIST）开发的“细胞分选显微镜系统”及其技术，对单细胞雨生红球藻（Heamatococcus pluvialis）生产虾青素和面包酵母酿酒酵母（Saccharomyces cerevisiae）生产硫胺素进行了研究。最新的荧光原位杂交（FISH）技术从明尼苏达大学（UMN）组转移到NAIST组，以及凝胶微滴技术，用于流式细胞术进行活细胞分选。实际上，工作人员和学生从一个部分被派到另一个部分。在逗留期间有小的sminars。学生们作为住院医师在实验室里呆了几个星期，1999年和2001年是NAIST派代表去的年份。2000年，UMN派代表在田纳西大学教授的陪同下前往MAIST参加联合研讨会。来自大坂和广岛大学的教授也被邀请。代表团的四名学生在NAIST的实验室逗留了三个星期，在SRIENC教授的帮助下，在两份主要的国际期刊上发表了两篇关于单细胞分析和细胞分选方法的评论论文。每个项目的成果报告也在编写之中。

This project was done in a research projection programme as an international cooperation between university institutions with official agreement for exchange programme. The research was for development of screening of organisms with specific cell functions or specific products, regardless of microorganisms, plants, and animals. On-going researches on the both sides were used as the model systems. Cytometry was the main methodology for the researches. "Cell sorting microscope system" and its technology, developed by the Nara Institute (NAIST) group, were used tor the astaxanthm production with a unicellular alga Heamatococcus pluvialis and thiamin production with the bakers' yeast Saccharomyces cerevisiae. An up-to-date fluorescence in situ hybridisation (FISH) technique was transferred from the University of Minnesota (UMN) group to the NAIST group, as well as gel microdrdplet technique, to be used in flow cytometry for viable cell sorting.Practically, staff and students were sent from a part to the other part. There were small sminars during the stays. The students stayed for a few weeks in laboratories as the residents, lie years 1999 and 2001 were forthe NAIST to send the delegates. In 2000, the UMN sent the delegate accompanied by a professor from the University ofTennessee to the MAIST for the joint symposium. Professors from Osaka arid Hiroshima Universities Were also invited. The four students from the delegate stayed for three weeks in the laboratories at NAIST.With help of Professor SRIENC, two review papers on single-cell analysis and cell sorting methodologies were presented in two major international journals. Report of the results from every project were also in preparation.