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System for Screening for microorganisms with specific functions or specific microbial products

特定功能微生物或特定微生物产品筛选系统

基本信息

批准号：
11695050
负责人：
SHINMYO Atsuhiko
金额：
$ 2.43万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

This project was done in a research projection programme as an international cooperation between university institutions with official agreement for exchange programme. The research was for development of screening of organisms with specific cell functions or specific products, regardless of microorganisms, plants, and animals. On-going researches on the both sides were used as the model systems. Cytometry was the main methodology for the researches. "Cell sorting microscope system" and its technology, developed by the Nara Institute (NAIST) group, were used tor the astaxanthm production with a unicellular alga Heamatococcus pluvialis and thiamin production with the bakers' yeast Saccharomyces cerevisiae. An up-to-date fluorescence in situ hybridisation (FISH) technique was transferred from the University of Minnesota (UMN) group to the NAIST group, as well as gel microdrdplet technique, to be used in flow cytometry for viable cell sorting.Practically, staff and students were sent from a part to the other part. There were small sminars during the stays. The students stayed for a few weeks in laboratories as the residents, lie years 1999 and 2001 were forthe NAIST to send the delegates. In 2000, the UMN sent the delegate accompanied by a professor from the University ofTennessee to the MAIST for the joint symposium. Professors from Osaka arid Hiroshima Universities Were also invited. The four students from the delegate stayed for three weeks in the laboratories at NAIST.With help of Professor SRIENC, two review papers on single-cell analysis and cell sorting methodologies were presented in two major international journals. Report of the results from every project were also in preparation.

这一项目是在一个研究预测方案中完成的，该方案是大学机构之间的国际合作，并就交流方案达成了正式协议。这项研究是为了筛选具有特定细胞功能或特定产品的生物体，而不考虑微生物、植物和动物。以双方正在进行的研究作为模型系统。细胞计量学是研究的主要方法。用奈良研究所(NAIST)开发的“细胞分选显微镜系统”及其技术，用单细胞藻雨生球藻生产虾青素，用面包酵母酿酒酵母生产硫胺素。最新的荧光原位杂交(FISH)技术从明尼苏达大学(UMN)小组转移到NAIST小组，以及凝胶微阵列技术，用于流式细胞术活细胞分类。在逗留期间有一些小矿工。学生们在实验室里呆了几个星期，因为1999和2001年的住院医生是NAIST派来的代表。2000年，UMN派代表在田纳西大学一名教授的陪同下前往Maist参加联合研讨会。来自大阪和广岛大学的教授也被邀请。该代表的四名学生在NAIST的实验室停留了三个星期。在SRIENC教授的帮助下，两篇关于单细胞分析和细胞分类方法的综述论文发表在两个主要的国际期刊上。每个项目的结果报告也在编写中。