Role of Na~+ in Plant Cells

Na~ 在植物细胞中的作用

• 批准号: 12440225
• 负责人: MIMURA Tetsuro
• 金额: $ 8.58万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12440225/
• 关键词: Cell membrane; Tonoplast; Characeae; Mangrove; Barley; Salt tolerance; Inorganic ion metabolism; Na^+; H^+ antiporter; プロトンATPアーゼ; プロトンATPアーぜ; Na^+環境認識; 液胞膜; H^+アンチポーダー

It is well known that Na^+ is not an essential element for most plants. Except for the salt tolerance, therewas almost no work on Na^+ metabolism, Na^+ transport in plant cells. Recently, we have found that Na^+ may play some important roles in plant life, too. In the present study, we focused roles of Na^+ in plant cell functions. We mainly investigated about Na^+ membrane transport systems, intracellular distribution of Na^+ and recognition of Na^+ environment by plant cells.We have found that under Pi deficiency, Chara cells induced Na^+ driven Pi uptake system in the plasma membrane and its physiological transport mechanism was analyzed. Furthermore, we found that the induction of Na^+/Pi cotransport system absolutely needs the presence of extracellular Na^+. Second, under K^+ deficiency, we found that Na^+ is playing an important role for plant growth by being accumulated in the vacuole. Lastly, we found the vacuolar volume quickly increased when mangrove cells are exposed to the salt stress. In this phenomenon, Na^+ transport was partly mediated with the vesicle transport into the vacuole.

众所周知，Na~(++)不是大多数植物的必需元素。除耐盐性外，对植物细胞内Na~+代谢、Na~+~(2+)转运几乎没有影响。最近，我们发现Na~(++)在植物生命中也可能起着重要的作用。在本研究中，我们重点介绍了Na~+在植物细胞功能中的作用。本研究主要从Na~+膜转运系统、Na~+在细胞内的分布以及植物细胞对Na~+环境的识别等方面进行了研究，发现在PI缺乏的情况下，轮状细胞诱导质膜上Na~+驱动的PI吸收系统，并对其生理转运机制进行了分析。此外，我们还发现，Na~(++)/PI共转运系统的诱导完全需要胞外Na~(++)的存在。其次，在K~(++)缺乏的情况下，我们发现Na~(++)通过在液泡中积累而对植物的生长起着重要的作用。最后，我们发现当红树林细胞暴露在盐胁迫下时，液泡体积迅速增加。在这一现象中，Na~+的转运部分是通过小泡向液泡的转运来实现的。

项目成果

Reid R.J.: "Phosphate transport in Chara : membrane transport via Na/Pi cotransport."Plant Cell & Environment. 23. 223-228 (2000)

Reid R.J.：“Chara 中的磷酸盐转运：通过 Na/Pi 共转运进行膜转运。”植物细胞

Yamada A: "Expression of mangrove allene oxide cyclase enhances salt tolerance in Escherichia coli, yeast, and tobacco cells"Plant & Cell Physiology. 43. 903-910 (2002)

山田 A：“红树丙二烯氧化物环化酶的表达增强了大肠杆菌、酵母和烟草细胞的耐盐性”植物

Mimura T.: "Induction of the Na^+/Pi co-transport system in the plasma membrane of Chara corallina requires external Na^+ and low levels of Pi"Plant Cell & Environment. 25. 1475-1481 (2002)

Mimura T.：“珊瑚藻质膜中 Na^/Pi 共转运系统的诱导需要外部 Na^ 和低水平的 Pi”Plant Cell

Mimura T.: "Rapid increase of vacuolar volume in response to salt stress"Planta. 16. 397-402 (2003)

Mimura T.：“响应盐胁迫，液泡体积迅速增加”Planta。

Kura-Hotta M., Mimura M., Tsujimura T., Nemoto-Washitani S., Mimura T.: "High salt treatment-induced Na^+ extrusion and low salt treatment-induced Na^+ accumulation in suspension-cultured cells of the mangrove plant, Bruguiera sexangula"Plant Cell & Envri

Kura-Hotta M.、Mimura M.、Tsujimura T.、Nemoto-Washitani S.、Mimura T.：“红树林悬浮培养细胞中高盐处理诱导的 Na^ 挤出和低盐处理诱导的 Na^ 积累