Development of Wide-area Fluorescent Image Observing System for Analysis of the Relationship between Function and High-dimension Structure in Biological System.

开发广域荧光图像观测系统,用于分析生物系统功能与高维结构的关系。

基本信息

项目摘要

Recent fluorescent microscopy is working as an extremely powerful tool in biological science. Combining the newly developed fluorescent probes with an improved fluorescent microscope, we are now able to observe not only morphological structure, but also biophysical situation like intracellular distribution of Ca^<2+> and pH,etc. Furthermore, in plant cells, auto fluorescence of chlorophyll has been used to understand the various photosynthetic phenomena.In the present study, we planed to develop a new-type fluorescence observing system. This is an apparatus that equips a computer-controlled wide area irradiation system and high-sensitive video camera. Using this system, we will be able to observc a fluorescent imagc of wide area like a stereoscopic microscope.If we use newly developed fluorescent probes (such as BCECF,Fura 2, etcf.) with a wide-area fluorescence viewing systcm, we will be able to detect an interaction between cells in a tissue besides intracellular reactions in the individual cell.We basically planned the system and constructed the irradiation system and the fluorescent observing system in 1993. In 1994 and 1995, we improved the following points.(1) To detect the auto fluorescence of chlorophyll under saturated irradiation, the irradiation system with Xenon lump was improved. Furthermore, a halogen irradiation system was added to illuminate the sample.(2) Computer-controlled exchange system of filters was added to the irradiation system.(3) Video camera controlling system was improved to expand the detection range.(4) Computer program was developed for the fluorescent image analysis.(5) The irradiation system was improved to be applicable for the ordinal fluorescence microscope.(6) As the preliminary experiments, a fluorescent induction fo chlorophyll and non-photo chemical quenching was observed using this system.This apparatus is still under construction. We need further improvements of both hard and soft wares.
近年来,荧光显微镜在生物科学中成为一种非常有力的工具。将新开发的荧光探针与改进的荧光显微镜相结合,我们现在不仅可以观察到形态结构,还可以观察到细胞内Ca^<2+>和pH等生物物理情况。此外,在植物细胞中,叶绿素的自荧光已被用来了解各种光合现象。在本研究中,我们计划开发一种新型荧光观测系统。这是一种配备电脑控制的广域辐射系统和高灵敏度摄像机的设备。使用该系统,我们将能够像立体显微镜一样观察到大面积的荧光图像。如果我们使用新开发的荧光探针(如BCECF,Fura 2等)和广域荧光观察系统,除了单个细胞内的细胞内反应外,我们将能够检测组织中细胞之间的相互作用。1993年,我们对系统进行了基本规划,建成了辐照系统和荧光观测系统。在1994年和1995年,我们改进了以下几点:(1)为了检测饱和辐照下叶绿素的自荧光,对氙块辐照系统进行了改进。此外,还增加了卤素照射系统来照射样品。(2)在辐照系统中增加了计算机控制的过滤器交换系统。(3)改进了摄像机控制系统,扩大了探测范围。(4)开发了荧光图像分析的计算机程序。(5)改进了辐照系统,使其适用于有序荧光显微镜。(6)初步观察到该体系对叶绿素的荧光诱导和非光化学猝灭。这台设备仍在建造中。我们在硬件和软件方面都需要进一步改进。

项目成果

期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shimmen, T., Mimura T., Kikuyama M., Tazawa M.: "Characean cells as a tool for studying electro-physiological characteristics of plant cells." Cell Struct. & Funct.19. 263-278 (1994)
Shimmen, T.、Mimura T.、Kikuyama M.、Tazawa M.:“Characean 细胞作为研究植物细胞电生理特征的工具。”
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Shimmen, T., Hamatani M., Saito S., Yokota E., Mimura T., Fusetani N., Karaki H.: "Roles of actin filaments in cytoplasmic streaming and organization of transvacuolar strands in root hair cells of Hydrocharis." Protoplasma. (in press).
Shimmen, T.、Hamatani M.、Saito S.、Yokota E.、Mimura T.、Fusetani N.、Karaki H.:“肌动蛋白丝在 Hydrocharis 根毛细胞的细胞质流动和跨液泡链组织中的作用。”
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Beilby, M.J.: "The proton pump,high pH channels, and excitation: voltage clamp studies of intact and perfused cells on Nitellopsis obtusa." Protoplasma. 175. 144-152 (1993)
Beilby, M.J.:“质子泵、高 pH 通道和激发:对 Nitellopsis obtusa 上完整和灌注细胞的电压钳研究。”
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Shimmen, T.: "Characean cells as a tool for studying electrophysiogical characteristics 0f plant cells." Cell Struct. &Funct.19. 263-278 (1994)
Shimmen, T.:“Characean 细胞作为研究植物细胞电生理特性的工具。”
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Mimura, T., Shimmen, T.: "Characterization of the Ca^<2+>-dependent C1^- efflux in perfused Chara cells" Plant & Cell Physiol. 35. 793-800 (1994)
Mimura, T.,Shimmen, T.:“灌注轮藻细胞中 Ca^2 依赖性 C1^- 流出的表征” Plant
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MIMURA Tetsuro其他文献

Changes in secondary metabolites related to plant development in Catharanthus roseus.
与长春花植物发育相关的次生代谢产物的变化。
  • DOI:
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    0
  • 作者:
    UZAKI Mai;K. YAMAMOTO Kotaro;TAKAHASHI Katsutoshi;OHNISHI Miwa;KURITA Yuko;SHICHIJO Chizuko;NAGANO Atsushi;ISHIZAKI Kimitsune;FUKAKI Hidehiro;MIMURA Tetsuro
  • 通讯作者:
    MIMURA Tetsuro
Analysis of lipids accumulated in laticifer and idioblast cells in Catharanthus roseus
长春花乳汁管和成纤维细胞脂质积累的分析
  • DOI:
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0
  • 作者:
    UZAKI Mai;YAMAMOTO Kotaro;TAKAHASHI Katsutoshi;OHNISHI Miwa;ISHIZAKI Kimitsune;FUKAKI Hidehiro;MIMURA Tetsuro
  • 通讯作者:
    MIMURA Tetsuro
Cell-specific analysis of localization of secondary metabolites in medicinal plant.
药用植物次生代谢物定位的细胞特异性分析。
  • DOI:
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    MIMURA Tetsuro;YAMAMOTO Kotaro;UZAKI Mai;OHNISHI Miwa;MIZUNO Hajime;MASUJIMA Tsutomu;TAKAHASHI Katsutoshi
  • 通讯作者:
    TAKAHASHI Katsutoshi

MIMURA Tetsuro的其他文献

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{{ truncateString('MIMURA Tetsuro', 18)}}的其他基金

Evolutional study on the membrane co-transport system in plant cell.
植物细胞膜协同转运系统的进化研究。
  • 批准号:
    25650128
  • 财政年份:
    2013
  • 资助金额:
    $ 6.02万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Studies on the mechanism of ion distribution and re-translocation in plant tissues.
植物组织中离子分布和重转运机制的研究。
  • 批准号:
    19370018
  • 财政年份:
    2007
  • 资助金额:
    $ 6.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Role of vacuolar network for ion homeostasis in plant cells under environmental adaptation.
环境适应下植物细胞离子稳态中液泡网络的作用。
  • 批准号:
    16085204
  • 财政年份:
    2004
  • 资助金额:
    $ 6.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Role of Na~+ in Plant Cells
Na~ 在植物细胞中的作用
  • 批准号:
    12440225
  • 财政年份:
    2000
  • 资助金额:
    $ 6.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
STUDIES ON THE ROLE OF VACUOLES IN ION METABOLISMS IN PLANT.
液泡在植物离子代谢中作用的研究。
  • 批准号:
    09044211
  • 财政年份:
    1997
  • 资助金额:
    $ 6.02万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
STUDIES ON THE ROLE OF VACUOLES IN ION METABOLISMS IN PLANT.
液泡在植物离子代谢中作用的研究。
  • 批准号:
    08044198
  • 财政年份:
    1996
  • 资助金额:
    $ 6.02万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Studies on Pi homeostasis by artificial manipulation of intracellular Pi levels.
通过人工调控细胞内 Pi 水平来研究 Pi 稳态。
  • 批准号:
    07640856
  • 财政年份:
    1995
  • 资助金额:
    $ 6.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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Measurement and modeling of solar-induced chlorophyll fluorescence in tropical forest
热带森林中太阳引起的叶绿素荧光的测量和建模
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    22H00554
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    2022
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    $ 6.02万
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Estimation of photosynthetic activity using solar induced chlorophyll fluorescence in a larch forest: Linkage of modeling and observations
利用落叶松林中太阳诱导的叶绿素荧光估算光合作用活动:建模与观测的联系
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    21K17873
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    2021
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    $ 6.02万
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SAMBA – Solar-induced chlorophyll fluorescence (SIF) in the AMazon BAsin
SAMBA â 亚马逊盆地中的太阳诱导叶绿素荧光 (SIF)
  • 批准号:
    446439624
  • 财政年份:
    2020
  • 资助金额:
    $ 6.02万
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    Research Grants
Chlorophyll fluorescence systems for real-time monitoring of plant performance
用于实时监测植物性能的叶绿素荧光系统
  • 批准号:
    RTI-2021-00777
  • 财政年份:
    2020
  • 资助金额:
    $ 6.02万
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    Research Tools and Instruments
MSA: Dynamics of Chlorophyll Fluorescence and Its Relationship with Photosynthesis from Leaf to Continent: Theory Meets Data
MSA:叶绿素荧光动力学及其与从叶子到大陆的光合作用的关系:理论与数据的结合
  • 批准号:
    1926488
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    2019
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    $ 6.02万
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    Standard Grant
Comprehensive analysis on the estimation potential of photosynthesis by solar induced chlorophyll fluorescence
太阳诱导叶绿素荧光估算光合作用潜力的综合分析
  • 批准号:
    18H03350
  • 财政年份:
    2018
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    $ 6.02万
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    Grant-in-Aid for Scientific Research (B)
Prediction of cellular metabolism by chlorophyll fluorescence
通过叶绿素荧光预测细胞代谢
  • 批准号:
    16H04809
  • 财政年份:
    2016
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Remote sensing of chlorophyll fluorescence using Unmanned Aerial Vehicles (UAVs)
使用无人机 (UAV) 遥感叶绿素荧光
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    289370018
  • 财政年份:
    2016
  • 资助金额:
    $ 6.02万
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    Research Fellowships
Development of a technique for monitoring the accumulation level of foreign protein in leaves using chlorophyll fluorescence
开发利用叶绿素荧光监测叶片中外源蛋白质积累水平的技术
  • 批准号:
    26712021
  • 财政年份:
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AirLIFT – an airborne active chlorophyll fluorescence sensing system for assessment of photosynthetic activity in plant canopies
AirLIFT — 一种空气传播的活性叶绿素荧光传感系统,用于评估植物冠层的光合作用活动
  • 批准号:
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  • 财政年份:
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